Module 5 Flashcards

1
Q

Compounds added to biological specimens to prevent them from clotting or to preserve
the constituents of a specimen.

A

additives

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2
Q

Any substance that prevents blood from clotting

A

anticoagulant

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3
Q

A prenatal test to detect birth defects that is performed at an early stage of pregnancy
and involves retrieval and examination of tissue from the organ.

A

chorionic villi sampling

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4
Q

The sequential process by which the multiple coagulation factors of blood interact in the
coagulation cascade, resulting in formation of an insoluble fibrin clot.

A

coagulation or clotting

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5
Q

Variation that occurs in the amount of a substance during a 24-hour period.

A

diurnal variation

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6
Q

Disruption of the red cell membrane causing release of hemoglobin and other components of red blood cells.

A

hemolysis

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7
Q

Disruption of the red cell membrane causing release of hemoglobin and other components of red blood cells.

A

hemolysis

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8
Q

The individual drawing a specimen of blood.

A

phlebotomist

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9
Q

The puncture of a blood vessel to collect blood.

A

phlebotomy

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10
Q

Factors that affect specimens before tests are performed and that can lead to error if not controlled; they are classified as controllable or uncontrollable.

A

pre-analytical factors

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11
Q

A substance or preparation added to a specimen to prevent changes in the constituents
of a specimen.

A

preservatives

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12
Q

The non-cellular component of anticoagulated whole blood; plasma contains clotting factors.

A

plasma

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13
Q

The watery portion of blood that remains after coagulation has occurred and can be obtained after centrifugation

A

serum

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14
Q

Collection of capillary blood usually from a pediatric patient by making a thin cut in the
skin, usually at the heel of the foot.

A

skin puncture

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15
Q

Obstruction of the return of venous blood to the heart and distention of the veins; in phlebotomy, this is a temporary blockage caused by application of pressure, usually from
a tourniquet.

A

venous occlusion

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16
Q

usually the specimen of choice

A

venous blood

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17
Q

Aside from venipuncture, blood samples can be collected using a skin puncture technique that can be done on

A

heel stick
middle or fourth finger
earlobe

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18
Q

uses both the liquid portion of the blood called plasma and the cellular components

A

whole blood

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19
Q

cellular components of blood

A

RBC
WBC
platelets

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20
Q

type of whole blood that is oxygenated and have uniform composition throughout the body

A

arterial blood

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21
Q

arterial blood is used in measuring:

A

pH

arterial blood gas

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22
Q

why is syringe used in arterial blood collection instead of evacuated tubes

A

because of the pressure in the arteries

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23
Q

primary arterial sites

A

radial
brachial
femoral

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24
Q

primary arterial sites

A

radial
brachial
femoral

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25
hazards of arterial collection
arterial pressure difficulty in stopping bleeding hematoma
26
disadvantages of arterial blood
technically difficult more painful not used for routine tests
27
advantages of arterial blood
blood gas analysis best for respiratory function high exygen content consistency
28
samples are deoxygenated and have variable composition
venous blood
29
direct puncture to collect venous blood is often located in the
antecubital area
30
venous blood differs from arterial blood in the concentration of
chloride, glucose, pH, CO2, lactic acid, ammonia
31
advantages of venous blood
good indicator easier to obtain used most frequently
32
disavantages of venous blood
minor and serious complications lack of accessible veins fear of needles
33
content of capillary blood
arterial blood venous blood tissue fluid
34
Point-of-care testing is often performed using this
capillary blood
35
capillary blood has higher
glucose
36
capillary blood has lower
Ca, K, total protein concentrations
37
advantages of capillary blood
small amounte needed simple and painless can change sites
38
disadvantages of capillary blood
not all tests can rupture blood cells calcofied nodules may develop in site
39
percentage of blood that is plasma
55%
40
percentage of plasma that is water
92%
41
other components of plasma
7% proteins | 1% mineral salts, sugar, fat, hormone, vitamins
42
serum is absent of
Factor I, V, VIII, XIII | 1 5 8 13
43
how long does blood clot with thrombin
5 minutes
44
how long does blood clot with clot activator
30 mins
45
how long does blood clot without clot activators
60 minutes
46
serum is centrifuged for
10 minutes 1000-2000 RCF
47
No interference from fibrinogen, anticoagulants and cations.
serum
48
Prone to fibrinogen, anticoagulant and cation interferences
plasma
49
Has a lower viscosity since it lacks fibrinogen
serum
50
Higher viscosity
plasma
51
Lower volume yield after centrifugation
serum
52
15-30% higher yield
plasma
53
Prone to interferences due to post centrifugal coagulation in serum
serum
54
No interference due to subsequent coagulation
plasma
55
Time consuming
serum
56
Time saving
plasma
57
fibrinogen interferes with
protein electoprophoresis
58
offers slight advantage in analysis of immunoglobulins due to improved specimen delivery and decreased precipotates
serum
59
next most common fluid for determination in the clinical chemistry laboratory.
urine
60
urine sample is more advantageous as
24-hour sample
61
CSF can be used for
protein and sugar determinatiolactate and other enzymes
62
capillary blood contains a higher portion of ____ because ____
arterial blood | pressure
63
capillary blood becomes even more similar to arterial blood in composition when the area is ____ because ___
warmed | increases blood flow in the area
64
higher in capilllary blood than in venous blood
glucose
65
lower in capillary blood than in venous blood
Ca, K, total protein
66
in infants, what is lost for every 10 mL of blood
4 mg of iron
67
life is threatened if more than ____ is removed at once
10% of patient's blood
68
The behavior of a healthcare provider toward, or as perceived by a patient
bedside manner
69
In general, specimens for determining the concentration of body constituents should be collected when the patient is in a
basal state
70
These are variables with short-lived effects that can be controlled or modified.
extrinsic or controllable variables
71
may occur due to prolonged application of tourniquet
hemoconcentration
72
hemoconcentration increases
protein, calcium, bilirubin, potassium
73
Serum and plasma show visual evidence of hemolysis when hemoglobin concentration exceeds
50 mg/dL
74
hemolysis increase or decrese | AST
increase
75
hemolysis increase or decresase | acid phosphatase
increase
76
hemolysis increase or decresase | lactate dehydrogenase
increase
77
hemolysis increase or decresase | potassium
increase
78
hemolysis increase or decresase | magnesium
increase
79
hemolysis increase or decresas | phosphate
increase
80
hemolysis increase or decresase | LDH
increase
81
hemolysis increase or decresase | sodium
decrease
82
hemolysis increase or decresase | calcium
decrease
83
hemoglobin absorbance peaks occur at
417 nm 540 nm 575 nm 415 nm absorbs l8ghte very strongly
84
free hemoglobin with its pseudoperoxidase activity interferes in bilirubin determination by inhibiting the diazonium color formation this causes biliribin to
inhibitory effect decrease
85
cortisol must be collected around
6-8 am
86
supine to upright position causes
hemoconcentration
87
If the patient is from a supine position then shifts to an inclined position (seated), allow the patient to at least be seated for ___ before specimen collection
20 minutes
88
ereft to supine position may cause
hemodilution
89
prolonged standinb increases concentration of
potassium creatininen kinase aldolase
90
prolonged standing decreases
plasma protein
91
lavender
EDTA
92
pink
EDTA
93
white
EDTA and gel
94
light blue
sodium citrate
95
red & gray
clot activator and gel
96
green
lithium heparin
97
light green
lithium heprin and gel
98
orange
thrombin
99
gray
potassium oxalate | sodium fluoride
100
tan
k2edta
101
royal blue
sodium heparin
102
yellow
SPS | sodium polyanethol sulfonate
103
black
sodium citrate
104
hematology
lavender
105
blood bank
pink
106
molecular diagnostics
white
107
coagulation studies
light blue
108
chemistry
green
109
chemistry lead tests
tan
110
chemistry trace elements and toxicology
royal blue
111
microbiology blood cultures
yellow
112
hematology erythrocyte sedimentation rates
black