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HSTCYT1 > H AND E STAINING > Flashcards

H AND E STAINING Flashcards

1
Q

major steps in routine H and E

A
  1. De-paraffinization
  2. Hydration
  3. Nuclear staining
  4. Differentiation/ decolorization
  5. Bluing
  6. Counterstaining
  7. Dehydration
  8. Clearing
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2
Q

reagent commonly used for bluing

A

ammonia water

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3
Q

composition of ammonia water

A
  • H2O
  • ammonium hydroxide
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4
Q

color of cartilage

A

Pink or light blue to dark blue

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5
Q

color of plasma cell and osteoblast

A

purplish pink

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6
Q

color of RBC, eosinophilic granules, keratin

A

bright orange red

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7
Q

color of cytoplasm

A

pink

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8
Q

color of nuclei

A

blue or blue-black

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9
Q

color of karyosome

A

dark blue

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10
Q

color of calcified bones

A

purplish blue

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11
Q

color of muscle fibers for H and E

A

deep pink

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12
Q

types of hematoxylin stain

A

A. aluminum hematoxylin solution
B. iron hematoxylin

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13
Q

recommended for progressive staining of tissue

A

aluminum hematoxylin solution

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14
Q

produces good nuclear stain

A

aluminum hematoxylin solution

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15
Q

mordant of ammonium hematoxylin solution

A

potash aluminum

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16
Q

for tissues subjected to acid decalcification

A

Erlich’s hematoxylin

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17
Q

for tissues that have been become acidic during prolonged storage in formalin

A

Erlich’s hematoxylin

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18
Q

ripening agent of Erlich’s hematoxylin

A

sodium iodate

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19
Q

stabilizer of Erlich’s hematoxylin

A

glycerine

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20
Q

2 solutions of Erlich’s hematoxylin

A
  • hematoxylin in absolute alcohol solution and heated
  • aluminum potassium sulfate in distilled water + glycerin and heated
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21
Q

used for routine nuclear staining, exfoliative cytology, and sex chromosomes

A

Harris’ Hematoxylin

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22
Q

staining time for Harris’ Hematoxylin

23
Q

ripening agent for Harris’ Hematoxylin

A

Mercuric oxide

24
Q

color of Harris hematoxylin when ripened with mercuric chloride

A

dark purple

25
stabilizer of Harris hematoxylin
4% glacial acetic acid
26
used in sequence with celestine blue
Cole's heamtoxylin
27
ripening agent of Cole's hematoxylin
Sodium perbonate
28
prior to use, it should be filtered before use for long periods
Cole's hematoxylin
29
used to demonstrate the presence of cytoplasmic glycogen by special stain
Mayer's hematoxylin
30
regressive and progressive staining
Meyer's Hematoxylin
31
used for the demonstration of cytoplasmic glycogen
Mayer's hematoxylin
32
ripening agent for Mayer's hematoxylin
sodium iodate
33
used mainly for differential or regressive staining
iron hematoxylin
34
uses acid alcohol as differentiator
iron hematoxylin
35
types of iron hematoxylin
1. weigert's hematoxylin 2. Heidenhain's hematoxylin 3. tungsten hematoxylin 4. copper hematoxylin
36
made up of ferric chloride and standard iron hematoxylin
weigert's hematoxylin
37
used in demonstrating muscle fibers and connective tissues
weigert's hematoxylin
38
combined with Van Gieson's stain: collagen
weigert's hematoxylin
39
mordant of Weigert's hematoxylin
ferric chloride
40
how many days will weigert's hematoxylin remain active
1-2 days
41
It changes color from a deep blue-black violet, through violet, purple, brown, and yellowish brown
weigert's hematoxylin
42
popular cytologic stain, especially for the study of mitosis
Heidenhain's hematoxylin
43
recommended for mitochondria, muscle striation, chromatin, and myelin
Heidenhain's hematoxylin
44
mordant for heidenhain's hematoxylin
Ferric Ammonium sulfate
45
variant of Mallory's PTAH
Tungsten hematoxylin
46
recommended for staining muscle striation
Tungsten hematoxylin
47
used for the study of spermatogenesis
Copper hematoxylin
48
routinely used as counterstain after hematoxylin and before methylene blue
eosin
49
red acidic dye in 3 forms
- Eosin Y - Eosin B - Eosin S
50
alcohol soluble eosin
Eosin S
51
Yellowish and most commonly used eosin
Eosin Y
52
Color of nuclei
Blue to blue black
53
Color of decalcified bone matrix, collagen, osteoid
Pink

HSTCYT1 (18 decks)

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