Lab Fixation

Question 1

Preparation, processing, and staining of tissue sections for microscopic study to be interpreted by the pathologist

Answer 1

Histotechniques

Question 2

Study of disease at the tissue level

Answer 2

Histopathology

Question 3

Removal of cells from the area of abnormality

Answer 3

Fine needle aspiration

Question 4

Considered as the simplest and least invasive method of collecting biopsy specimens

Answer 4

Fine needle aspiration

Question 5

Method of collection for fluid-containing tumors

Answer 5

Fine needle aspiration

Question 6

Removal of cells and small amount of surrounding tissue

Answer 6

Core needle biopsy

Question 7

Removal of cells and small amount of surrounding tissue

Answer 7

Core needle biopsy

Question 8

Removal of cells with more surrounding tissue; for dermatological samples

Answer 8

Incisional biopsy

Question 9

Removal of the entire area in question

Answer 9

Excisional biopsy

Question 10

Removal of 3-4mm cylindrical core of tissue samples

Answer 10

Punch biopsy

Question 11

Measurement of small punch biopsy

Answer 11

2mm

Question 12

Measurement of large punch biopsy

Answer 12

4mm

Question 13

Removal of small fragments of tissue from a surface

Answer 13

Shave biopsy

Question 14

Removal of tissue or growths from body cavities

Answer 14

Curettings

Question 15

Where shoul the lesion be for punch biopsy

Answer 15

Center of area in question

Question 16

Scoon or spoon sample

Answer 16

Curettings

Question 17

Superior than clinical lab test

Answer 17

Biopsy

Question 18

Storage of specimen

Answer 18

1month to 1 year

Question 19

Storage time of tissue blocks

Answer 19

3-10 years

Question 20

Storage time for slides

Answer 20

Indefinite

Question 21

Storag etime for records (request and result forms)

Answer 21

Permanent

Question 22

What types of dyes are used to stain fresh tissue examination

Answer 22

Supravital or differential

Question 23

What types of microscopes are used to examine fresh tissue examination

Answer 23

Brightfield or phase contrast microscope

Question 24

No fixative is required

Answer 24

Fresh tissue examination

Question 25

Advantages of fresh tissue examination

Answer 25

-observation of physiologic processes or protoplasmic activities (motion, mitosis, phagocytosis, pinocytosis) -relatively simple and easy to perform

Question 26

Disadvantages of fresh tissue examination

Answer 26

-limited use -liable to develop changes observed after death (putrefaction and autolysis)

Question 27

Dissection or separation of tissue components in NSS or Ringer's solution

Answer 27

Teasing

Question 28

Examined as stained or unstained and anatomical relationship is destroyed

Answer 28

Teasing

Question 29

Tissue is sandwhiched between2 sides

Answer 29

Squash

Question 30

Stain is applied on one side of th slide and allowed to spread

Answer 30

Squash

Question 31

What type of spreading action is observed in squah preparation

Answer 31

Capillary action

Question 32

For cytological studies, especially for th diagnosis of cancer

Answer 32

Smearing

Question 33

For sections and sediments, performed using a wire loop, applicator stick or another slide

Answer 33

Smearing

Question 34

Uniform distribution in a zigzag manner

Answer 34

Streaking

Question 35

For thick or mucoid specimens which involves teasing on a slide

Answer 35

Spreading

Question 36

Maintains intercellular relationship

Answer 36

Spreading

Question 37

For the preparation of blood and bone marrow smears

Answer 37

Pull-apart

Question 38

For thick secretions resulting to an even distribution of sample

Answer 38

Pull-apart

Question 39

One side of a slide is allowed to touch a surface of the sample

Answer 39

Touch preparation

Question 40

Preparedusing freezing microtome or cryostat

Answer 40

Frozen section

Question 41

For rapid diagnosis

Answer 41

Frozen section

Question 42

Specimen accessioning or identification is performed by

Answer 42

Medical technologist

Question 43

Gross examination and sampling is performed by

Answer 43

Pathologist

Question 44

osmolality of light microscopy

Answer 44

slightly hypertonic (400-450 mOsm)

Question 45

osmolality of electron microscopy

Answer 45

more or less isotonic (340 mOsm)

Question 46

what does tap water remove

Answer 46

-chromates -formalin -osmic acid

Question 47

decolorizer of mercuric chloride

Answer 47

5% na thiosulfate

Question 48

decolorizer of melanin

Answer 48

potassium permanganate KMnO4

Question 49

what reduces melanin

Answer 49

pyrogallic acid

Question 50

bleach and final removal of melanin

Answer 50

H2O2