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METALLIC FIXATIVES Flashcards

1
Q

Types of metallic fixatives

A

A. Mercuric chloride
B. Chromate fixatives
C. Lead fixatives

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2
Q

Most common metallic fixative

A

Mercuri chloride

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3
Q

Widely used as secondary fixative

A

Mercuric chloride

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4
Q

What color precipitate may mercuric chloride produce

A

Black

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5
Q

What type of metallic fixative is for tissue photography and preserve very fine details of tissue and provide high quality image

A

Mercuric chloride

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6
Q

Out of all the mercuric chloride fixative which one does not produce a black precipitate

A

Heidenhein’s SuSa

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7
Q

Mercuric chloride is satisfactory for:

A
  • trichome staining
  • immunoperoxidase techniques
  • fixative of choice for preserving cell details in tissue photography
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8
Q

Zenker’s Fluid is composed of:

A

Mercuric chloride solution and Glacial acetic acid

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9
Q

For small pieces of liver, spleen, connective tissue fibers, and nuclei

A

Zenker’s Fluid

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10
Q

Fixation time for Zenker’s fluid

A

12 - 24 hours

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11
Q

Other name for zenker-formol

A

Helly’s solution

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12
Q

For fixing pituitary gland, bone marrow, and blood containing organs

A

Zenker-Formol or helly’s solution

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13
Q

For fixing cytoplasmic granules

A

Zenker-Formol

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14
Q

Fixation time for zenker-formol

A

12-24 hours

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15
Q

Excellent cytologic fixative

A

Heidenhein’s SuSa

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16
Q

For tumor biopsies of the skin

A

Heidenhein’s SuSa

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17
Q

Fixation time for SuSa

A

3-12 hours

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18
Q

Used for bone marrow biopsies

A

B-5 fixative

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19
Q

Fixation time of B-5 fixatives

A

1, 1/2-2 hours (fastest fixation time)

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20
Q

Concentration of Chromic acid

A

1-2%

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21
Q

Constituent of compound fixatives

A

Chromic acid

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22
Q

Precipitates all proteins and adequately preserves carbohydrate

A

Chromic acid

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23
Q

Percentage of the concentration of Potassium Dichromate

A

3%

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24
Q

It ia a chromative fixativ that preserves lipids

A

Potassium Dichromate

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25
It is a type of chromate fixative that preserves mitochondria
Potassium Dichromate
26
PH of mitochondria in Potassium dichromate
4.5-5.2
27
Other name for Regaud's Fluid
Muller's Fluid
28
Recommended for the demonstration of chromatin, mitochondria, mitotic figures, golgi bodies, RBC, and colloid containing tissues
Regaud's Fluid
29
Fixation time for Regaud's fluid
12-48 hours
30
What type of chromate fixative is recommended for early degenerative processes and tissue necrosis
Orth's Fluid
31
Chromate Fixative for the demostration of Rickettsiae and bacteria
Orth's Fluid
32
Fixation time for Orth's Fluid
36-72 hours (longer fixation time)
33
Used in 4% aqueous solution of basic lead acetate
Lead Fixatives
34
recommended for acid mucoploysaccharide and connective tissue mucin
Lead Fixatives
35
considered as both a fixative and a stain
Picric acid
36
normally used in strong saturated aqueous solution (1%)
Picric acid fixatives
37
dyes tissue yellow
Picric acid fixative
38
What is used to remove yellow dye from the picric acid
Acid dye or lithium carbonate
39
used an explosive agent by the military
Picric acid fixative
40
excellent fixative for glycogen demonstration
Picric acid fixatives
41
coagulant fixative and strong soagulant of nucleoproteins
Picric acid fixatives
42
picric acid fixative recommended for embryos and pituitary biopsies
Bouin's solution
43
fixation time for Bouin's solution
6-24 hours
44
Modification of Bouin's solution
Brasil's alcoholic picroformol fixative
45
better and less messy than Bouin's solution
Brasil's alcoholic picroformol fixative
46
excellent fixative for glycogen
Brasil's alcoholic picroformol fixative
47
Used in conjunction with other fixatives to form compound solution
Glacial HAc
48
Solidifies at17C
Glacial acetic acid
49
fixes and precipitates nucleoproteins
Glacial acetic acid
50
Causes tissues to swell; precipitates chromosomes and chromosome material
Glacial acetic acid
51
Rapidly denatures and precipitates proteins
Alcohol fixatives
52
Fix and preserve glycogen, pigments, blood, tissue films and smear
Absolute alcohol
53
ideal foro small tissue fragments and a fixative and a dehydrating agent at the same time
Alcohol fixatives
54
Excellent for glycogen preservation and preserves nuclear stain
Alcohol fixatives
55
An alcohol fixative that fixes dry and wet smear, blood smear and bone marrow tissue
Methyl alcohol
56
Used for fixing touch preparations
Isopropyl alcohol
57
May be used as simple fixatives
Ethyl alcohol
58
Recommended for fixing mucopolysaccharide and nuclear protein
Newcomer's fluid
59
fixation time of ethyl alcohol
18-24 hours
60
Fxation time of newcomer's fluid
12-18 hours at 3C
61
most rapid alcohol fixative
Carnoy's Fluid
62
Used for urgent biopsies and brain tissue for diagnosis of rabies
Carnoy's fluid
63
Fixation time for carnoy's fluid
1-3 hours
64
how many hours is urgent biopsy processed?
5 hours
65
Pale yellow powder which dissolves in water
Osmium tetroxide
66
Other name for osmium tetroxide
Osmic acid
67
fixes conjugated-fats and lipids permanently
Osmium tetroxide
68
Preserves cytoplasmic structures well (mitochondria and golgi bodies)
Osmium tetroxide
69
fixes myelin and peripheral nerves well and adequately fixes materials fo ultra thin sectioning
Osmium tetroxide
70
Most common chrome-osmium acetic acid and fixative
Flemming's solution
71
Osmium tetraoxide fixative that is Recommended for nuclear strucures such as chromosomes
Flemming's solution
72
It permanently fixes fats
Flemming's solution
73
Fixation time for flemming's solution
24-48 hours
74
osmium tetraoxide fixative that is not for nuclear structures
Flemming's solution without acetic acid
75
Mad up of only chromic and osmic acid
Flemming's solution without acetic acid
76
Type of osmium tetroxide fixative that is recommended for cytoplasmic structures such as the mitochondria
Flemming's solution without acetic acid
77
Fixation time for Flemming's solution without acetic acid
24-48 hours
78
Type of alcohol fixative that is used for fixing touch preparations
Isopropyl alcohol (95%)
79
Fixativ that is sometimes incorporated into compound fixatives
Trichloroacetic acid
80
Precipitates proteins
Trichloroacetic acid
81
Has a marked swelling effect
Trichloroacetic acid
82
May be used as a weak decalcifying agent
Trichloroaetic acid
83
Used at ice cold temperature ranging from -5 to 4C
Acetone
84
Recommended for phosphates and lipases (water diffusable agents)
Acetone
85
Used for fixing brain tissues for diagnosing rabies (2nd fixative for brain tissues and diagnosing rabies)
Acetone
86
Involves thermal coagulation of tissue proteins
Heat fixation
87
Employed for frozen tissue sections and bacteriological smear
Heat fixation
88
Preserves nuclear and cytoplasmic detail
Heat fixation

HSTCYT1 (18 decks)

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