MOUNTING

Question 1

refractive index of glass

Answer 1

1.518

Question 2

mounting media which contains xylene

Answer 2

Resinous media

Question 3

R.I. of DPX

Answer 3

1.532

Question 4

R.I. of XAM

Answer 4

1.52

Question 5

tree where Canada balsam is extracted

Answer 5

Abus balsamea

Question 6

R.I. of Canada balsam

Answer 6

1.524

Question 7

Resinous media for small tissue and dry rapidly

Answer 7

DPX

Question 8

Recommended for whole mounts and is one of the best mounting media

Answer 8

Canada balsam

Question 9

Mounting media for lipid which does not require xylene

Answer 9

Aqueous media

Question 10

R.I. of water

Answer 10

1.33

Question 11

Aqueous media for temporary mounting which has low refractive index

Answer 11

Water

Question 12

Standard mounting medium for fat stains

Answer 12

Glycerin jelly

Question 13

Glycerin jelly is also known as

Answer 13

Kaiser’s 1880

Question 14

RI of glycerin jelly

Answer 14

1.47

Question 15

Composition of glycerin jelly

Answer 15

• gelatin
• glycerol
• dH2O
• phenol crystals

Question 16

Other name of Gum Arabic

Answer 16

Farrant’s

Question 17

RI of Gum Arabic

Answer 17

1.43

Question 18

Composition of Gum Arabic

Answer 18

• Gum Arabic
• dH2O
• glycerol
• sodium merthiolate

Question 19

RI of apathy’s medium

Answer 19

1.52

Question 20

Composition of apathy’s medium

Answer 20

• gum Arabic
• sucrose
• thymol
• dH2O

Question 21

aqueous media for frozen sections

Answer 21

Brun’s fluid

Question 22

other aqueous media

Answer 22

• permount
• HSR
• Clearmount

Question 23

what is used to remove stain on skin

Answer 23

remove by using 0.5% acid alcohol –> tap water

Question 24

how to restain old sections

Answer 24

shortcut:
“X - XhKhOhR”
- slide - xylene - remove coverslip - xylene - K2MnO4 - oxalic acid - restain

Question 25

sealing the margins of the coverslip

Answer 25

ringing

Question 26

purpose of ringing

Answer 26

- prevent escape/evaporation of fluid - immobilize the coverslip - prevent sticking of slides

Question 27

what are the 2 adhesives used for ringing

Answer 27

a. Kronig cement b. Durofix (cellulose adhesive)

Question 28

what is the cellulose adhesive for ringing

Answer 28

Durofix

Question 29

composition of Kronig cement

Answer 29

2 parts paraffin + 4-9 parts colophonium resin

Question 30

purpose of mounting

Answer 30

- protect specimen from physical injury - protect specimen from deterioration - preserve for permanent keeping - handle and store samples easier

Question 31

Criteria for good mounting media

Answer 31

- RI must be as close as the RI of glass - should not dry quickly - should not dissolve or fade tissue - should not cause shrinkage or distortion of tissue - should be able to set hard for permanent mounting