Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

HSTCYT1 > MICROTOMY > Flashcards

MICROTOMY Flashcards

1
Q

previously processed tissue / tissue block is trimmed and cut into uniformly thin slices or sections

A

Microtomy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

principle of microtomy

A

a spring balanced teeth or pawl is brought into contact with, and turns a ratchet feed wheel connected to a micrometer screw, which is in turn rotated, moving the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

when was rocking (cambridge) microtome invented

A

1881

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

who invented the rocking (cambridge) microtome

A

Paldwell Trefall

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q
  • the simplest type of microtome for small and large paraffin-embedded blocks
A

rocking microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

thickness of rocking microtome

A

10-12u

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

one of its disadvantage is, it will not form serial sections

A

rocking microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

part of the rocking microtome that carries the block holder

A

upper arm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

part of the rocking microtome that supports the column

A

lower arm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

commonly used microtome for paraffin-embedded sections

A

rotary microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

allows production of excellent serial sections

A

rotary

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

heavier and more stable microtome with up and down vertical movement which results to perfectly flat planes

A

rotary

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

who invented the rotary m

A

minot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

when was the rotary microtome invented

A

1885-86

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

thickness of rotary m

A

4-6u

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

2 types of sliding m

A
  1. standard sliding
  2. base-sledge
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

the thickness of sliding m

A

4-9u

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

who invented the sliding m

A

Adams

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

when was the sliding m invented?

A

1789

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

sliding m that consists of 2 movable pillars holding the adjustment knife clamps for hard tissues and large blocks

A

base sledge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

sliding m that is prone to danger

A

standard sliding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

block remains stationary, the microtome moves in a forward and back motion

A

standard sliding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

for hard and tough tissue blocks in all forms of media

A

base-sledge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

for electrom microscope

A

ultra thin microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
thickness of ultra-thin m
0.5u
26
uses fragments of broken plate glass
ultrathin microtome
27
who invented the freezing microtome
Queckett
28
when was the freezing microtome invented
1848
29
for undehydrated tissues in frozen state
freezing microtome
30
for rapid diagnosis and for fats and tissue constituents that may be damaged
freezing microtome
31
thickness of freezing m
10-15u
32
source of cold temp for freezing microtome
carbon dioxide
33
sections to be focused under compound microtome
1. plane-concave 2. plane-wedge 3. biconcave
34
for paraffin and celloidin embedded sections
plane-concave
35
length of plane-concave
25mm
36
for celloidin-embedded sections and for extremely hard tissues
plane-wedge
37
length of plane-wedge knife
100mm
38
for rotary microtome and for paraffin-embedded sections
biconcave
39
Knives: sections to be focused under EM
1. Diamond-edge 2. glass knives
40
resin blocks for UTSM and for EM; knife is expensive
diamond-edge
41
for clearing and semi-thin sections
glass knives
42
Used for modern lab, cheaper and convenient, composed of conventional steel knives
Disposable blades
43
Microtome knives angle
- clearance angle - wedge angle - bevel angle - rake angle
44
Between the edge of the knife and the tissue block
Clearance angle
45
Angle of cutting
Wedge angle
46
Angle of cutting facet
Bevel angle
47
(CA-BA) - 90°
Rake angle
48
Angle of CA
5-15°
49
Angle of WA
15°
50
Angle of BA
27-32°
51
Tests for microtome knife shapening
- should cut a paraffin wax block at 2-4 um thickness w/o serrations when examined under the microscope (100x) - von mhol's criterion - will split a hair drawn across it with only their own resistance
52
Test that confirm shapening of knife is good when there is a Straight narrow, continuous life reflected from the knife edge until the other end if light is focused on the knife
Von Mhol's criterion
53
To remove nicks and irregularities
Honing
54
Hones
- Carborundum - arkansas - Belgium yellow - Belgian black vein
55
Most expensive hone
Belgium yellow
56
For rough side of honing
- carborundum - arkansas
57
For smooth side of honing
Belgium yellow
58
T/f the finer the grain the harder the hone
T
59
Used for final polishing
Diamantine
60
After repeated sharpening, widened bevel angle (more than 35°)
Factory grinding
61
T/f hones utilizes oilstones
T
62
Material for honing
Smooth stones and machine hone
63
To remove burrs and for final polishing
Stropping
64
Lubricant for honing
- soapy water, oil (mineral oil, castor oil, clove oil) or xylene
65
Lubricant for stropping
Vegetable oil
66
Direction for honing
Heel to toe
67
Direction for stropping
Toe to heel
68
Motion and no. Of strokes for honing
-zigzag - 10-20 single strokes
69
Motion and no. Of strokes for stropping
-zigzag - 40-120 double strokes
70
Flatten tissues in water bath at 10C lower than MP of paraffin wax
Floating
71
What is used for adhesion
Mayer's egg albumin
72
Composition of Mayer's egg albumin
-egg white - glycerin - thymol
73
Transfer of tissue sections/ribbons on the slide using camel's hair brush/applicator stick
Fishing out
74
Correct positioning of the tissue section or ribbon on the slide
Orientation
75
General steps in fixing sections onto slide
1. Floating 2. Adhesion 3. Fishing out 4. Orientation 5. Deparaffinization 6. Drying sections 7. Post- mordantinh
76
To remove excess paraffin
Deparaffinization
77
Equipment for deparaffinization
- alcohol lamp - paraffin oven
78
Tissue will adhere to glass slide for long duration for staining
Drying sections
79
Equipment for drying sections
- wax oven - incubator -hot plate - alcohol lamp/ bunsen burner
80
Temp for wax oven for drying sections
56-60 C for 2 hrs
81
Temp for hot plate for drying sections
45-55C for 30-45 mins (fastest)
82
To improve the demo of elements in the tissue in staining
Post- mordanting
83
Secondary fixation(post-chroming) and used primarily as mordant and secondary fixative
Post - mordanting

HSTCYT1 (18 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions