Hct, Pcv, Blood Smear Review

Question 1

Normal pcv for dogs?

Answer 1

37-55%

Question 2

Normal pcv of cats?

Answer 2

35-45%

Question 3

What is the white called between the rbcs and plasma of hematocrit tubes?

Answer 3

Buffy coat

Question 4

What do you use the refractometer to find out?

Answer 4

Total protein

Question 5

What is the of unit of total protein?

Answer 5

g/100ml

Question 6

Normal plasma total protein of dogs and cats?

Answer 6

6.0-7.5 g/dL

Question 7

What do you do before using a refractometer?

Answer 7

Calibrate with distiller water

Question 8

What is normal plasma color?

Answer 8

Straw yellow

Question 9

What does white plasma mean?

Answer 9

Lipemic-lipids in blood.

Question 10

What does yellow plasma mean?

Answer 10

Icteric-liver is having problems

Question 11

What does red/pink plasma mean?

Answer 11

Hemolysis- ruptured rbcs, heat, freezing, small gauge needle

Question 12

What do we look for in blood smears?

Answer 12

Confirm cell numbers, differential count, cell morphology, blood borne parasites

Question 13

Best tube to do a blood smear with?

Answer 13

EDTA, purple top

Question 14

Why do we use purple tops for blood smears? (Cbc)

Answer 14

Preserves blood morphology best

Question 15

Steps of blood smears?

Answer 15

Clean, dry slide, mix sample well, put a small drop on the slide, hold the spreader at 40 degrees, back the spreader into the drop, push the spreader forward, smooth feathered edge

Question 16

Why do we not apply pressure during blood smears?

Answer 16

May rupture/squish cells

Question 17

What should the smear look like?

Answer 17

A thumb with a feathered edge

Question 18

What happens if you increase the angle of the blood smear pusher?

Answer 18

It will be a thicker smear

Question 19

What happens if you decrease the angle of the blood smear pusher?

Answer 19

Make a thinner smear

Question 20

What are the here distinct areas of a blood smear?

Answer 20

Thick blood cell area, red cell area, feathered edge

Question 21

What do we use the thick blood cell area for?

Answer 21

Nothing really

Question 22

What do we use the red cell area of a smear for?

Answer 22

To view morphology, differential- they’re spaced out well

Question 23

What do we use the feathered edge of a smear for?

Answer 23

To check for clumped platelets and heartworm microfilariae

Question 24

If the smear is too short and thick?…

Answer 24

Make the drop smaller and decrease spreader angle

Question 25

If the blood smear is too thin...?

Answer 25

Increase blood drop, increase angle of spreader and apply less pressure

Question 26

Steps to dying blood smear?

Answer 26

Let air dry!, stain (fixative-blue), eosin stain (red), methylene blue (dark blue), rinse with water, air dry

Question 27

How long do we have to make a slide and then to stain?

Answer 27

3 hours after collecting, one hour after slide is made

Question 28

What is the best way to scan a smear?

Answer 28

Zig zag pattern

Question 29

What you see when you look into the microscope?

Answer 29

Field of view

Question 30

What are we looking for with red blood cell morphology?

Answer 30

Shape and size normal?

Question 31

What are we looking for with white blood cell morphology?

Answer 31

Neutrophils mature? Lymphocytes normal? Wbc inclusions?

Question 32

What are we looking for for platelet morphology?

Answer 32

Normal numbers? Normal size?

Question 33

The percentage of whole blood that Is composed of red blood cells.

Answer 33

Hematocrit, packed blood cell volume

Question 34

What do total proteins tell us?

Answer 34

Amount of proteins in the blood- especially proteins regarding liver

Question 35

If we have an anemic patient, what happens to our pcv?

Answer 35

Lower packed cell volume

Question 36

If our patient is dehydrated,what happens to our pcv?

Answer 36

Increased

Question 37

If our patient is dehydrated, what will happen to our total protein?

Answer 37

Increase, it's not spread out

Question 38

Here parts of a blood slide?

Answer 38

Thick body area, red cell rainbow counting area, feathered edge