Hct, Pcv, Blood Smear Review Flashcards Preview

Hematology > Hct, Pcv, Blood Smear Review > Flashcards

Flashcards in Hct, Pcv, Blood Smear Review Deck (38):
1

Normal pcv for dogs?

37-55%

2

Normal pcv of cats?

35-45%

3

What is the white called between the rbcs and plasma of hematocrit tubes?

Buffy coat

4

What do you use the refractometer to find out?

Total protein

5

What is the of unit of total protein?

g/100ml

6

Normal plasma total protein of dogs and cats?

6.0-7.5 g/dL

7

What do you do before using a refractometer?

Calibrate with distiller water

8

What is normal plasma color?

Straw yellow

9

What does white plasma mean?

Lipemic-lipids in blood.

10

What does yellow plasma mean?

Icteric-liver is having problems

11

What does red/pink plasma mean?

Hemolysis- ruptured rbcs, heat, freezing, small gauge needle

12

What do we look for in blood smears?

Confirm cell numbers, differential count, cell morphology, blood borne parasites

13

Best tube to do a blood smear with?

EDTA, purple top

14

Why do we use purple tops for blood smears? (Cbc)

Preserves blood morphology best

15

Steps of blood smears?

Clean, dry slide, mix sample well, put a small drop on the slide, hold the spreader at 40 degrees, back the spreader into the drop, push the spreader forward, smooth feathered edge

16

Why do we not apply pressure during blood smears?

May rupture/squish cells

17

What should the smear look like?

A thumb with a feathered edge

18

What happens if you increase the angle of the blood smear pusher?

It will be a thicker smear

19

What happens if you decrease the angle of the blood smear pusher?

Make a thinner smear

20

What are the here distinct areas of a blood smear?

Thick blood cell area, red cell area, feathered edge

21

What do we use the thick blood cell area for?

Nothing really

22

What do we use the red cell area of a smear for?

To view morphology, differential- they're spaced out well

23

What do we use the feathered edge of a smear for?

To check for clumped platelets and heartworm microfilariae

24

If the smear is too short and thick?...

Make the drop smaller and decrease spreader angle

25

If the blood smear is too thin...?

Increase blood drop, increase angle of spreader and apply less pressure

26

Steps to dying blood smear?

Let air dry!, stain (fixative-blue), eosin stain (red), methylene blue (dark blue), rinse with water, air dry

27

How long do we have to make a slide and then to stain?

3 hours after collecting, one hour after slide is made

28

What is the best way to scan a smear?

Zig zag pattern

29

What you see when you look into the microscope?

Field of view

30

What are we looking for with red blood cell morphology?

Shape and size normal?

31

What are we looking for with white blood cell morphology?

Neutrophils mature? Lymphocytes normal? Wbc inclusions?

32

What are we looking for for platelet morphology?

Normal numbers? Normal size?

33

The percentage of whole blood that Is composed of red blood cells.

Hematocrit, packed blood cell volume

34

What do total proteins tell us?

Amount of proteins in the blood- especially proteins regarding liver

35

If we have an anemic patient, what happens to our pcv?

Lower packed cell volume

36

If our patient is dehydrated,what happens to our pcv?

Increased

37

If our patient is dehydrated, what will happen to our total protein?

Increase, it's not spread out

38

Here parts of a blood slide?

Thick body area, red cell rainbow counting area, feathered edge