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BACTERIOLOGY > I: Gram Negative Bacilli > Flashcards

I: Gram Negative Bacilli Flashcards

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1
Q

Enterobacteriaceae are

CPON NO GF FA

A

Catalase Positive
Oxidase Negative
Nitrate Reduction Positive
Glucose Fermentation
Facultative Anaerobe

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2
Q

Enterobacteriaceae are MOTILE; EXCEPT FOR (2)

A

Klebsiella and Shigella

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3
Q

Type of Flagella present in Enterobacteriaceae

A

Peritrichous Flagella

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4
Q

Listeria and Y. Enterocolitica are motile in ___ and NON-MOTILE IN ________

A

Motile in ROOM TEMPERATURE
Non Motile 37 Deg Celsius

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5
Q

Enterobacteriaceae are Catalase (+) EXCEPT

A

Shigella ; S.dysenteriae

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6
Q

Enterobacteriaceae can reduce Nitrate to Nitrite EXCEPT

A

Erwinia
Pantonea agglomerans

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7
Q

Antigenic Structure:

Heat-LABILE
Capsular Antigen
Carrier

A

K Antigen

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8
Q

Antigenic Structure:

Salmonellae have capsular (K) antigen known as

A

Vi antigen

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9
Q

Antigenic Structure:

Somatic Antigen
Cell wall
Heat-STABLE

A

O antigen

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10
Q

Antigen Structure:

Flagella
denatured by Alcohol/heat
agglutinate with anti-H antibodies

A

H Antigens

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11
Q

O antigen, antibodies are predominantly

A

IgM (Acute/Active Infection)

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12
Q

H antigens, antibodies are predominantly

A

IgG (past infection)

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13
Q

Rapid Lactose Fermenters

A

EKE

Escherichia
Klebsiella
Enterobacter

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14
Q

Late Lactose Fermenters

A

Cit Ser Yer Haf Sa Shir is late

Citrobacter
Serratia
Yersinia enterocolitica
Hafnia
Salmonella arizonae
Shigella sonnei

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15
Q

Non-Lactose Fermenters

A

Sobrang Sarap PEPE Mo

Shigella
Salmonella
Proteus
Edwardsiella
Providencia
Erwinia
MOrganella

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16
Q

Rapid Lactose Fermenters that causes

UTI - 90%
Sepsis
Meningitis - Infants
Diarrheal Disease

A

Escherichia

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17
Q

Rapid Lactose Fermenters

Most common cause of UTI in humans

A

UPEC
Uropathogenic E.coli

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18
Q

Rapid Lactose Fermenters

UPEC virulence factors (3)

A

Pili
Cytolysins
Aerobactin

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19
Q

Rapid Lactose Fermenters

UPEC: VF that is responsible for adherence to epithelial cells

A

Pili

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20
Q

Rapid Lactose Fermenters

UPEC: VF that is responsible for inhibiting IMMUNE EFFECTOR CELLS

A

Cytolysin

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21
Q

Rapid Lactose Fermenters

UPEC: VF that is responsible for chelating iron

A

Aerobactin

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22
Q

Rapid Lactose Fermenters

Escherichia that Produces HEAT LABILE/HEAT STABLE TOXINS that resides in Plasmid

A

ETEC
Enterotoxigenic E.coli

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23
Q

Rapid Lactose Fermenters

ETEC:
toxin that similarly related with CHOLERA TOXIN

A

Labile Toxin

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24
Q

Rapid Lactose Fermenters

ETEC:
toxin that result to intestinal fluid secretion by stimulating GUANYLATE CYCLASE

A

Stable Toxin

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25
Rapid Lactose Fermenters Type of E.coli that causes: Montezuma's Revenge Traveler's diarrhea Childhood diarrhea
Enterotoxigenic E.coli (ETEC)
26
Rapid Lactose Fermenters Type of E.coli Invades Intestinal Epithelium causing SHIGELLA-LIKE INFECTION
EIEC EnteroInvasive E.coli
27
Rapid Lactose Fermenters Causes Dysentery in young Children living in areas with of POOR SANITATION
EIEC EnteroInvasive E.coli
28
Rapid Lactose Fermenters EIEC is characterized with Explosive stool; due to acid ______ with the presence of _____ CFU/mL
1. Acid SENSITIVITY 2. 100,000 CFU/mL
29
Rapid Lactose Fermenters E.coli characterized with stool with RBC, NEUTROPHILS and mucus
EIEC EnteroInvasive E.coli
30
Rapid Lactose Fermenters Type of E.coli: - Non-invasive - No toxin - Nosocomial (Newborn and Infants)
EPEC Enteropathogenic E.coli
31
Rapid Lactose Fermenters Type of E.coli: - Characterized with Watery diarrhea with mucus but W/O BLOOD
EPEC Enteropathogenic E.coli
32
Rapid Lactose Fermenters Type of E.coli that produces Verotoxin, toxin that resembles S. DYSENTERIAE
EHEC/VTEC Enterohemorrhagic E.coli Verotoxic E.coli
33
It has a cytotoxic effect on the Vero cells of the kidney of African green monkey
Verotoxin
34
Most Severe manifestation of EHEC/VTEC
HUS Hemorrhagic Uremic Syndrome
35
E.coli Most frequently associated with E.coli O157:H7
EHEC/VTEC
36
Only strains that is NEGATIVE on SMAC (Sorbitol Macconkey Agar)
Colorless E.coli O157:H7
37
Rapid Lactose Fermenters E.coli that involves: Binding Pili Stable toxin like Hemolysin like Causes: Watery diarhea
EAEC Enteroaggregative E.coli
38
Rapid Lactose Fermenters Exhibit mucoid growth; Large Polysaccharide capsule
Klebsiella
39
Rapid Lactose Fermenters Klebsiella spp; - Friedlander's bacillus - Encapsulated - Mucoid colonies
K. pneumoniae
40
Rapid Lactose Fermenter Klebsiella spp. - purulent sinus infection
K. Ozaenae
41
Rapid Lactose Fermenter Klebsiella spp. - granuloma in nose and nasophranyx
K. rhinoscleromatis
42
Rapid Lactose Fermenter Klebsiella spp. is associated with ESBL (Extended Spectrum Beta-Lactamase) T/F
True
43
Rapid Lactose Ferments Associated with Opportunistic infections: UTI, RT, Wound infections
Enterobacter
44
Rapid Lactose Fermenter Enterobacter: Most predominant Isolate
E. cloacae
45
Rapid Lactose Fermenter Enterobacter that produces yellow pigmentation that intensifies at 25 deg C.
E. sakazakii ( now: Chromobacter sakazakii )
46
LOA + - -
K. pneumoniae K. oxytocoa S. typhi
47
LOA + + -
E. Aerogenes
48
LOA Neg Pos Pos
E. cLoacae
49
LOA - - -
P. agglomerans
50
Outbreak of septicemia resulting from CONTAMINATED INTRAVENOUS FLUID
Pantoea agglomerans
51
Pantoea agglomerans serotype that produces yellow pigment; PRIMARILY A PLANT PATHOGEN
Pantoea agglomerans HG XII
52
Triple Decarboxylase Negative
E. agglomerans
53
Triple Enzyme (+) - DNAse - Lipase - Gelatinase
Serratia
54
Serratia is ONPG (+) EXCEPT:
S. fonticola
55
Serratia spp. S. marcescens, S. rubidaea, S.plymuthica produces pink-red pigment due to _____ when incubated at Room Temperature
PROGIOSIN
56
- Mostly significant Serratia - Infection of Urinary and Respiratory - Bacteremic outbreaks - Septic arthritis
S. marcescens
57
Serratia associated with OSTEOMYELITIS found following motorcycle accident
S. plymuthica
58
Serratia emits dirty, musty odor like that of potatoes
S. odorifera
59
Tribe Salmonellae
Salmonellae Citrobacter Arizonae
60
Organisms resemble Salmonella but ONPG positive and LDC negative
Citrobacter
61
- Citrobacter associated with Endocarditis in Intravenous drug abusers - 80% produces Hydrogen Sulfide - 50% fail to ferment lactose
Citrobacter freundii
62
Salmonella and Citrobacter are differentiated through (2)
- Urea Hydrolysis - Lysine Decarboxylase
63
Salmonella VS Citrobacter Urea Hydrolysis (+) Lysine Decarboxylase (-) KCN Agar (+)
C. freundii
64
Salmonella VS Citrobacter Urea Hydrolysis (-) Lysine Decarboxylase (+) KCN Agar (-)
Salmonella
65
Citrobacter causes nursery outbreaks of neonatal miningitis and brain abcess
C. koseri
66
Sources of infection: Water, Milk, Dairy products, shellfish, dried or frozen eggs, meat, household pets
Salmonella
67
Bacteria that is acquired from water, dairy products, meats; - causes Enteritis, Systemic infection, Enteric Fever
Salmonella
68
Salmonella Serogroup A
S. paratyphi A
69
Salmonella Serogroup B
S. paratyphi B
70
Salmonella Serogroup C
S. cholerasuis
71
Salmonella Serogroup D
S. enterica serotype Typhi
72
Salmonella that causes bacteremia
S. cholerasuis
73
Most common manifestation of salmonella infection
Enterocolitis/Gastroenteritis
74
Salmonella spp that causes Enterocolitis/Gastroenteritis
S. typhimurium
75
Milk borne disease of Cow
S. typhi
76
Milk borne disease of man
M. bovis
77
Non-motile Salmonella spp.
S. pullorum S. gallinarum
78
Tube dilution agglutination test for SALMONELLA
Widal's Test
79
Widal's Test Specimen: Interval:
Specimen: 2 serum Interval: 7-10 days
80
Widal's Test High or Rising Titer of O > 1:160
Acute/Active Infection
81
Widal's Test High or Rising Titer of H > 1:160
Past Infection/Immunization
82
Widal's Test Highest titer of Vi antigen
Carriers
83
A state by which the individual recovers from infection but harbors the organisms in GALLBLADDER (chronic carriage)
Carrier State
84
The carrier state may be terminated by
Antimicrobial Therapy
85
Widal's Test (Carrier State Specimen) 1st Week 2nd Week 3rd Week
1st Week - Blood 2nd Week - Urine/Stool 3rd Week - Serum
86
Gold Standard Specimen for Widal's Test
Bone marrow aspirate
87
Biochemically inert, natural habitat is limited to intestinal tracts of Humans. Produces BACILLARY DYSENTERY
Shigella
88
Shigella Stool with blood, mucus, pus
dysentery
89
Shigella Seizures, HUS, associated with S. dysenteriae
Shigellosis
90
SHIGELLA SPP Si Sonnei BC mag FB kaya na DA Catalase (-) ONPG (+)/late Mannitol (-)
Sonnei - Group type D Boydii - Group Type C Flexneri - Group Type B Dysenteriae - Group type A Catalase (-) - Dysenteriae ONPG (+)/late - Sonnei Mannitol (-) - Dysenteriae
91
Produce infections when they leave intestinal tract; UTI, Bacteremia, pneumonia, nosocomial Infections
Proteus
92
Proteus that exhibits swarming motility
P. mirabilis P. vulgaris
93
Part of PPM tribe that is associated with Satghorn stones, Struvite, Ammonium Mg Phosphate/ Renal Stone
Proteus
94
OX-2 OX-19 are strains from
P. vulgaris
95
OX-K are strains from
P. mirabilis
96
Proteus Acquired A phenomenon
P. mirabilis
97
Proteus Acquired B phenomenon
P. Vulgaris, E.coli 086
98
Proteus OX-K
Kingsburg
99
Swarming non-hemolytic
Proteus
100
swarming beta-hemoyltic
Clostridium septicum

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