II: Gram Negative Bacilli Flashcards
(111 cards)
1
Q
Normal Intestinal Flora
UTI associated
Often resistant to antimicrobial therapy
A
Providencia
2
Q
Providencia spp. urease positive
A
P. rettgeri
3
Q
Providencia spp. urease negative/variable
A
P. stuartii
4
Q
Proteus spp that is Indole (-)
A
P. mirabilis
5
Q
Proteus spp. Indole (+)
A
P. vulgaris
6
Q
- Gram-negative Bacilli
- Non-Lactose Fermenters
- Isolated from Cold blooded and warm blooded animals
A
Edwardsiella
7
Q
Edwardsiella spp. occurs in most human species
A
E. tarda
8
Q
E. tarda has same IMVIC result to
A
E.coli (++–)
9
Q
To differentiate E. tarda from E.coli
E. tarda has
H2S:
NLF / RLF:
A
H2S: +
NLF / RLF: NLF
10
Q
To differentiate E. tarda from E.coli
E.coli has
H2S:
NLF / RLF:
A
H2S: -
NLF / RLF: RLF
11
Q
Late-Lactose Fermenter
Gram-Negative Bacilli
Causes Black Death/Bubonic Plaque
A
Yersinia pestis
12
Q
Vector of Yersinia pestis
A
Xenopsylla cheopsis (Rat flea)
13
Q
Yersinia pestis in broth agar exhibits clump of cells adhere to one side of tube known as
A
Stalactite pattern
14
Q
Y. pestis preferred temp for growth
A
25-30 deg Cel
15
Q
Y. pestis exhibit ____ appearance after 48 hrs in BAP
A
Cauliflower
16
Q
Y. pestis inclusion bodies forms
A
safety pin appearance
17
Q
Y. pestis inclusion bodies are stained using
A
Wayson stain
18
Q
Gram-negative Bacilli
Late-Lactose Fermenters
Causes ENTEROCOLITIS (fever, abdominal pain, diarrhea); Appendicitis in children
A
Y. enterocolitica
19
Q
Associated with Arthitis, Erythrema Nodosum
Gram-negative Bacilli
Late Lactose Fermenters
A
Y. enterocolitica
20
Q
Y.enterocolitica is motile @ _______ deg and non motile @ ___________
A
Motile - Room Temp
Non-Motile - 37 deg Cel
21
Q
Y. enteroclotica is culture in CIN agar; CIN means
A
Cefsulodin Irgasan Novobiocin Agar
22
Q
Y. enterocolitica in CIN agar appears
A
Bull’s Eye colonies
23
Q
H2S (+)
SPACEd
A
Salmonella
Proteus
Arizonae
Citrobacter freundii
Edwardsiella
24
Q
Microorganism with Bipolar Inclusion Bodies
A
Bipolar si BAY
B. pseydomallei
A. actinomycetemcomitans
Yersinia
25
LISTAHAN NG MGA BULL-BULLL
Bull-eyes colonies on CIN
Y. Enterocolitica
Aeromonas
26
LISTAHAN NG MGA BULL-BULLL
Fish eye colonies on EMB
Enterobacter
27
LISTAHAN NG MGA BULL-BULLL
Bull's eye amoeba
E. histolytica
28
LISTAHAN NG MGA BULL-BULLL
Bull's Eye Rash
Borellia burdorgferi
29
LISTAHAN NG MGA BULL-BULLL
Bull's Neck Infection
C. diptheriae
30
LISTAHAN NG MGA BULL-BULLL
Buffalo Hump
Cushing Syndrome
31
Agar that uses Ferric Ammonium Citrate for indication of H2S
SSA, XLD, HEA, SIM , LIA
32
Considered as INITIAL STEP for the identification of Enterobacteriaceae
Triple Sugar Iron Agar (TSIagar)
33
TSI Agar Component:
CHO (3)
1% Lactose (Slant)
1% Sucrose (Slant)
0.1% Glucose (Butt)
34
TSI Agar component:
pH indicator
Phenol Red
35
TSI Agar component:
Sulfur Source:
Sodium thiosulfate
36
TSI Agar Component:
H2S indicator:
Ferrous sulfate
37
H2S + Ferric Ions =
Ferrous sulfide (black precipitate)
38
TSI Reactions spp.
A/AG H2S (-)
EKE
Escherichia
Klebsiella
Enterobacter
39
TSI Reactions spp.
K/AG H2S (+)
SPACEd
Salmonella
Proteus
Arizonae
Citrobacter freundii
Edwardsiella tarda
40
TSI Reactions spp.
K/A H2S (-)
Shigella
Providencia
Morganella
41
TSI Reactions spp.
K/K H2S (-)
Pseudomonas
Burkholderia
42
Test primarily for ENTERIC pathogen
Lysine Iron Agar (LIA)
43
test used to detect if organism can deaminate Lysine (red) or Decarboxylase Lysine (purple)
Lysine Iron Agar
44
Lysine Iron Agar Composition:
Amount of protein must be in:
a. Small
b. Large
Small amount of protein
45
Lysine Iron Agar Composition:
CHO
Glucose
46
Lysine Iron Agar Composition:
pH indicator
Bromcresol purple
47
Lysine Iron Agar Composition:
H2S indicator
Ferric Ammonium Citrate
48
LIA Reaction spp.
K/K H2S (+)
Salmonella
49
LIA Reaction spp.
K/A H2S (-)
Shigella
50
LIA Reaction spp.
R/A H2S (-)
PPM
Proteus
Providencia
Morganella
*Proteus
- H2S (+) but does deaminate lysine = alkaline = inc. ammonia = Alk cannot produce H2S since; H2S can only be produced in ACIDIC ENVIRONMENT
51
Selective Differential Media for Gram-Negative Enteric Bacilli (5)
1. Eosin Methylene Blue
2. Macconkey Agar
3. Hektoen Enteric Agar (HEA)
4. Salmonella Shigella Agar
5. XLD Agar
52
Eosin-Methylene Blue Agar Composition:
CHO:
Inhibitor & Indicator:
CHO: Lactose and Sucrose
Inhibitor & Indicator: Eosin Methylene Blue
53
Eosin-Methylene Blue Result:
Lactose Fermenters:
Non Lactose Fermenters:
LF: Pink to Purple Colonies
NLF: Colorless
54
Eosin-Methylene Blue Result:
Pink to Purple Colonies with Green Metallic Sheen
E.coli
55
Eosin-Methylene Blue Result:
Pink mucoid colonies
Klebsiella
56
Eosin-Methylene Blue Result:
Pink colonies with Black Center "Fish Eye Colonies"
Enterobacter
57
Macconkey Agar Composition:
Inhibitor
Crystal Violet
Bile Salt
58
Macconkey Agar Composition:
pH indicator
Neutral Red
59
Macconkey Agar Composition:
CHO
Lactose
60
Macconkey Agar Result:
NLF:
LF:
NLF: Colorless colonie
LF: Pink
61
Neutral Red
Acidic < 6.8
Alkaline 6.8 -8.0
Acidic < 6.8 = Pink-red
Alkaline 6.8 -8.0 = Brown
62
Hektoen Enteric Agar Composition:
CHO
Lactose, Sucrose, Salicilin
63
Hektoen Enteric Agar Composition:
Inhibitor
Bile Salts
64
Hektoen Enteric Agar Composition:
pH indicator
Bromthymol Blue
65
Hektoen Enteric Agar Composition:
H2S indicator
Ferric Ammonium Citrate
66
Hektoen Enteric Agar Result:
LF (H2S +)
NLF (H2S -)
LF (H2S +) - Yellow-Orange with Black Center
NLF (H2S -) - Green-Blue w/o Black Center
67
Salmonella-Shigella Agar Composition:
CHO
pH indicator
H2S indicator
CHO: Lactose
pH indicator: Neutral Red
H2S indicator: Ferric Ammonium Citrate
68
Salmonella-Shigella Agar Result:
Colorless Black Center
H2S +
NLF
Salmonella
69
Salmonella-Shigella Agar Result:
Colorless w/o Black Center
H2S -
NLF
Shigella
70
Salmonella will have Mauve-colored colonies; Enterobacter will have blue-white colonies
CHROMagar Salmonella
71
NLF spp. produces lysine decarboxylase showing Red Colonies w/ Black Center in this agar
XLD Agar
72
XLD agar composition:
CHO
Sulfur Source
pH indicator
H2S indicator
Inhibitor
CHO - Lactose, Sucrose, Xylose
Sulfur Source - Sodium thiosulfate
pH indicator - Phenol Red
H2S indicator - Ferric Ammonium Citratre
Inhibitor - Sodium desoxycholate
73
XLD agar Result:
Red w/ Black Center or Colorless w/ Black Center
Salmonella
74
IMVIC
Indole
Methyl Red
Voges Proskauer
Citrate
75
Indole detects for
Tryptophanase
76
Indole reagent
Kovacs/Ehrlich Reagent (PDAB)
77
Kovac's Reagent
Positive Control:
Negative Control:
Positive Control: E. coli
Negative Control: K. pneumoniae
78
Ehrlich Reagent
Positive Control:
Negative Control:
Positive Control: H. influenzae
Negative Control: H. parainfluenzae
79
Indole Test
Medium uses
Sulfur Indole Motility (SIM) / Tryptone Broth
80
Indole Test Result:
Positive:
Negative:
Positive: Red Ring
Negative: w/o color change
81
Indole Test Positive spp
May PEKPEC Po?
Morganella
Proteus vulgaris
E. tarda
K. oxytoca
Providencia
E. coli
Citrobacter koseri
Plesiomonas
82
Test for Mixed Acid Fermentation Pathway
Methyl Red
83
Methyl Red - Mixed Acid Fermentation = LAFS
Lactic
Acetic
Formic
Succinyl acid
84
Methyl Red QC:
Positive
Negative
Positive: E.coli
Negative: Enterobacter aerogenes
85
Methyl Red Result
Positive:
Negative:
Positive: Red
Negative: Yellow
86
Test used to detect Acetoin (ButyleneGlycol Pathway)
Voges-Proskauer Test
87
Voges Proskauer Reagent
a-naphtol (4drops)
40% KOH (2 drops)
88
Voges Proskauer QC:
Positive
Negative
Positive: Enterobacter aerogenes
Negative: E.coli
89
Voges Proskauer Result:
Positive
Negative
Positive: Red
Negative: Yellow
90
Voges-Proskauer Positive Spp.
KEESH
Klebsiella
Ewingella
Enterobacter
Serratia
Hafnia
91
Citrate Utilization Test Composition:
Medium:
pH indicator:
Simmon's Citrate
Bromthymol blue
92
Citrate Utilization Test Result:
Positive:
Negative:
Positive: Growth/Blue
Negative: No growth/ green
93
Citrate Positive Test:
Pero Sa CHEEKS
Pero Sa CHEEKS
Providencia
Serratia
Citrobacter
Erwingella
Enterobacter
Klebsiella
Salmonella enteritidis
94
Malonate utilization has the same result w/ (3)
Acetamide
Acetate
Citrate
95
Malonate Utilization Result
Positive
Negative
Positive: blue
Negative: green or yellow
96
Urease Test Composition
Medium:
pH indicator:
Positive will produce:
Medium: Stuart's Urea broth/ Christensen's Urea Agar
pH indicator: Phenol Red
Positive will produce: Ammonia
97
Urease Test
Rapid Urease will produce:
TPUNCH
Rapid Urease will produce: Pink to Red colonies w/in 2-4 hrs
TPUNCH
T. mentagrophytes
PPM
Ureaplasma
Nocardia
C. neoformans
H. pylori
98
Urease Test
Slow Urease will produce:
CKEYS
Slow Urease will produce: Orange after 4hrs
CKEYS
Citrobacter
Klebsiella
Enterobacter
Yersinia enterocolitica
Serratia
99
ONPG test is a rapid test to detect
B-galactosidase
100
ONPG substrate
O-nitrophenyl-beta-D-galactopyranoside
101
ONPG is hydrolyze to form O-nitrophenol forming
Yellow colonies (LF)
102
ONPG Result:
Positive:
Negative:
Positive: Yellow
Negative: Colorless
103
Test to detect B-gluconidase
MUG test
104
All E.coli are MUG TEST POSITIVE EXCEPT
E. coli 0157: H7
105
Test that detects the ability of an organims to remove carboxyl group from amino acid
Decarboxylase Test
106
Decarboxylase Test Composition:
Medium:
Indicator:
CHO:
Amino acid:
Medium: Decarboxylase Basal Medium (Moller's Medium)
Indicator: Bromcresol Purple
CHO: Glucose
Amino acid: Lysine, Ornithin, Arginine
107
Decarboxylase Test
Positive Result
Purple-yellow-purple
108
Test to detect organism's ability to remove amino group from an amino acid
Phenylalanine Deaminase
109
Phenylalanine Deaminase composition:
Phenylalanine Agar
Overnight Culture
10% FECI
Phenylalanine Agar
Overnight Culture
10% FECI3
110
Phenylalanine Deaminase Result
Positive
Green Slant and fluid
111
Routinely Performed Test for Enterobacteriaceae (6)
1. IMVIC, MOtility
2. Growth in KIA/TSI - LF, H2S
3. Urease, ONPG
4. LDC, ODC, ADC
5. PPA/PDA
6. Mannitol Fermentation
