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BACTERIOLOGY > Part II: Gram Positive Cocci (Streptococci) > Flashcards

Part II: Gram Positive Cocci (Streptococci) Flashcards

(54 cards)

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1
Q

Gram (+) cocci in chains/spherical to ovoid

A

STREPTOCOCCI

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2
Q

STREPTOCOCCI : Catalase

A

-

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3
Q

STREPTOCOCCI : Motility and Special Requirement

A

Non motile
Capnophilic

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4
Q

Starting point in Streptococci Identification:

A

Hemolysis in BAP

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5
Q

Selective Media of Streptococci

A

Phenyl ethyl agar (PEA)

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6
Q

Medium of choice in Streptococci

A

Sheep’s Blood Agar

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7
Q

presumptive test for CATALASE (-); GRAM (+) COCCI

A

LAP (LEUCINE AMINO PEPTIDASE) Test

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8
Q

LAP : Substrate

A

Leucine-beta-napthylamide

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9
Q

LAP: Reagent

A

Cinnamaldehyde

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10
Q

LAP:

(+)(-) Result

A

(+) Red
(-) no color/yellow

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11
Q

classifiesStreptococci according to hemolysis that they
produce on BAP

A

Smith and Brown Classification

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12
Q

Can cause incomplete/partial
hemolysis on BAP

Greening of Agar

A

Alpha hemolysis

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13
Q

Can cause complete
hemolysis on BAP

Clear zones around colonies

A

Beta Hemolysis

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14
Q

Does not cause
hemolysis

A

Gamma hemolysis

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15
Q
  • most significant
    in identifying and classifying Beta Hemolytic Streptococci;
A

LANCEFIELD CLASSIFICATION

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16
Q

based on the extraction of C
carbohydrate from the Streptococcal cell wall

A

LANCEFIELD CLASSIFICATION

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17
Q

A.k.a. Group A Beta hemolytic Streptococci

A

S. pyogenes

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18
Q

Known as major throat pathogen

A

S. pyogenes

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19
Q

S. pyogenes : Clinical Significance

A
  1. Pharyngitis
  2. Scarlet Fever
  3. Necrotizing fascitis
  4. Erysipelas
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20
Q

Known as flesh-eating bacteria

A

S. pyogenes

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21
Q

Continuous Streptococcal infection may lead to

A

Rheumatic fever
Acute Glomerulonephritis (AGN)

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22
Q

S. pyogenes: Media for Initial isolation

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A

Sheep’s blood agar

23
Q

S. pyogenes : PYR test

24
Q

PYR test reagent

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A

N-n-dimethylaminocinnamaldehyde

25
PYR test (+) Result
RED
26
S. pyogenes : PYR test Bacitracin Test Camp's Test Sulfamethoxazole Test
PYR test - POS Bacitracin Test - SUSCEPTIBLE Camp's Test - NEG Sulfamethoxazole Test - RESISTANT
27
S. pyogenes : VIRULENCE FACTOR ● Major virulence factor ● Prevents phagocytosis
M protein
28
S. pyogenes : VIRULENCE FACTOR Oxygen Labile Causes sub-surface hemolysis Occurs anaerobically Antigenic/Immunogenic
Streptolysin O
29
S. pyogenes : VIRULENCE FACTOR Oxygen stable Causes surface hemolysis Occurs aerobically Non-antigenic
Streptolysin S
30
S. pyogenes : VIRULENCE FACTOR ● Promotes attachment to ET (epithelial) cells
PROTEIN F
31
S. pyogenes : VIRULENCE FACTOR Once released, it causes rashes in scarlet fever
ERYTHROGENIC TOXIN/PYROGENIC TOXIN
32
S. pyogenes : VIRULENCE FACTOR Spreading factor
Hyaluronidase
33
S. pyogenes : VIRULENCE FACTOR Promotes fibrinolysis; dissolution of clots
Streptokinase
34
Detects immunity for Scarlet Fever
Dick's Test
35
Dicks Test (+) Result
Erythema (Redness in site of injection)
36
Test to confirm if the Erythema is caused by Scarlet fever or not
Shultz Charlton Test / Blanche Phenomenon
37
Shultz Charlton Test / Blanche Phenomenon (+) result
Fading of rashes within 6-8 hrs (MEANING: RASHES IS NOT CAUSED BY SCARLET FEVER)
38
SCHULTZ CHARLTON TEST involves injecting
Anti-erythrogenic toxin
39
Beta Hemolytic streptococci that is #1 caused of NEONATAL MENGITIS
S. agalactiae
40
S. agalactiae is known to cause ________ in adults
Postpartum endometritis
41
Method to detect genital carriage by S.agalactiae in pregnant woman
Todd Hewitt
42
Major Virulence Factor of S.agalactiae
Capsule
43
S.agalactiae is CAMP
CAMP (+)
44
CAMP test uses what media
Blood Agar Plate
45
CAMP TEST Known organism Unknown organism
Known organism: S. aureus Unknown organism: S. agalactiae
46
CAMP TEST Positive Result shows
Enhanced hemolysis (arrow head-beta hemolysis)
47
CAMP TEST Negative Result shows
No enhanced hemolysis
48
Test used for the detection of S.agalactiae that hydrolyze sodium hippurate by HIPPURICASE enzyme
Hippurate Hydrolysis test
49
When sodium hippurate is hydrolyzed what are the products formed? (2)
1. Benzoic Acid 2. Glycine
50
Hippurate Hydrolysis Indicator (2) 1. Benzoic Acid 2. Glycine
1. Benzoic Acid - Ferric Chloride 2. Glycine - Ninhydrin
51
Hippurate Hydrolysis Test that is more rapid
Glycine - Ninhydin
52
Hippurate Hydrolysis Test that is slow
Benzoic Acid - Ferric Chloride
53
S. agalactiae PYR: Bacitracin Susceptibility: SXT Susceptibility:
PYR: NEGATIVE Bacitracin Susceptibility: RESISTANT SXT Susceptibility: RESISTANT
54
CAMP TEST manner of streaking
perpendicular streak

BACTERIOLOGY (25 decks)

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