Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CAPS 390 > L1. introduction+Staining+Cell > Flashcards

L1. introduction+Staining+Cell Flashcards

1
Q

What is the diameter of the aorta

A

5cm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the cross sectional diameter of a small artery

A

1mm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is an organ?

A

group of tissues that are assembled into units that serve a broader function

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the diameter of a RBC?

A

7-8 um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the diameter of a typical cell?

A

10um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the diameter of a nuclei?

A

3um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the resolution for LM?

A

up to 0.25um are visible by LM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the resolution for EM?

A

anything less than 0.25um is visible through EM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the size of a mitochondria?

A

0.2um to 3um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the diameter for individual cytoskeletal filaments?

A

5nm -10nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what is the diameter of microtubules?

A

25nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the diameter of a DNA helix?

A

2nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the process for histology?

A
  1. fixation
  2. dehydration
  3. embedding
  4. sectioning
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the difference between the process for LM and EM?

A

LM: embedding in parrafin
sectioning is thick with 5-10um
EM: embedding in plastic/resin
sectioning is thin with 25-100nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the process of fixation?

A

cross-link proteins and macromolecules with aldehydes

maintains tissue architecture for microscopic sectioning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What are the different staining types?

A
  1. histochemical : based on charge low specificity
  2. immunochemical: based on antibodies binding to antigens; high specificity
  3. fluorescent tags: linked to various antibodies, facilitates optical sectioning
17
Q

What pH is eosin and hematoxylin?

A

eosin: pink (acidic)
hemotoxylin: blue ( basic)

18
Q

What does Osmium Tetroxide stain for? which kind of microscopy is it used in?

A

Binds with lipids.

Used in EM

19
Q

What microscopy can optically section an image

A

confocal microscopy : can take 3D images

CAPS 390 (35 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions