L8:2

Question 1

Following activation of CD4+ T Lymphocyte,
___ cytokine is made first

Answer 1

IL-2

Question 2

True or False: As antigen-stimulated T cells receive differing signals from the APC, they
differentiate into subsets of CD4+ T cells that are defined solely by cytokine production
profiles

Answer 2

True

Question 4

Th0 are probably a precursor to the ___, ___, and ___ phenotypes
and produce cytokines such as __, ___, and ___

Answer 4

Th1, Th2, and Th17
IL-2, IL-4, and interferon-gamma (IFN-γ)

Question 5

What two cytokines are made by T-helper-1 (Th1) cells?

Answer 5

IL-2 and IFN-γ

Question 6

Antigen stimulation of naive CD4+ T
cells in the presence of specific cytokines (IFN-γ and IL-12) drive T cells toward the ___
subset.

Answer 6

Th1

Question 7

What is the initial source of IL-12 and IFN-γ in Th1 cells?

Answer 7

Initial source of IL-12 = APC

Initial source of IFN-γ = NKC /
γδ TCR+ T cells.

Question 8

T-helper-2 (Th2) lymphocytes produce ___ and ___

Answer 8

IL-4 and IL-5

Question 9

When naive CD4+ T cells are
stimulated with antigen in the presence of IL-4, what do they develop into?

Answer 9

Th2 cell

Question 10

What is the initial source of
IL-4, early in driving the CD4+ Th0 cell to a Th2 phenotype?

Answer 10

mast cell

Question 11

What two cytokines influence an isotype switch in B cells to IgG1, IgG2, IgG4, IgA and IgE?

Answer 11

IL-4 and IL-5

Question 12

Which cell type influence
antibody-mediated or “humoral” immune responses (type 2 immune responses)?

Which cell type influences CTI Responses (Type 1 immune response?

Answer 12

Type II = Th2

Type I = Th1 cells

Question 13

Which cell type activates B cells and allows for their differentiation (plasma/memory)?

Answer 13

Tfh cells (follicular)

Question 14

Which cell is associated with CD25 and Fox P3+?

Answer 14

T Reg cells

Question 15

Which cell type allows for conversion
of B cells to IgG3?

Answer 15

Th1

Question 16

True or False: To make Tfh (follicular) cells,
they must develop in presence of IL-6, IL-21

Answer 16

True

Question 17

Which cell type recruits eosinophils/basophils?

Answer 17

Th2 cells

Question 18

Which cells regulate auto-immune response
and suppress other effector T cells?

Answer 18

T reg cells

Question 19

Which cells up-regulate NK cells/Th1 and activate CTL and macrophages?

Answer 19

Th1 cells

Question 20

True or False: Follicular cells express CD4+ and PD-1

Answer 20

True

Question 21

True or False: Th1 cells resolve diptheria, menigities, pneumonia and diptheria

Answer 21

False - Th2 cells does

Question 22

What cell type drives asthma/allergies?

Answer 22

Th2

Question 23

Which cell type drives RA, MS, Psoriasis?

Answer 23

Th17

Question 24

True or False: Natural T-Regs are specific for self antigens and develop WITHIN the thymus in presence of TGF-β, while Induced develop OUTSIDE of the thymus

Answer 24

True

Question 25

True or False: The type of antigen, molecular structure, HLA Type, and route of exposure will all determine which T cell phenotype will predominate

Answer 25

True

Question 26

CD8 binds to ____ domain of HLA Class I molecule

Answer 26

alpha-3

Question 27

__ is required for CD8+ proliferation and differentiation into CTL effectors

Answer 27

IL-2

Question 28

True or False: CD8+ depends on Th1 activation for proliferation and differentiation into CTL effectors

Answer 28

True

Question 29

In granular-mediated killing, CTL mobilizes cytoplasmic granules toward the target via: _____

Answer 29

actin filaments

Question 30

What molecules are located within the cytoplasmic granules present in granular mediated killing?

Answer 30

1) Perforin 2) TNF alpha/beta 3) Granzyme 4) Granulysin

Question 31

In granular mediated killing, how are granular contents released onto target cell membrane?

Answer 31

Exocytosis

Question 32

True or False: Pores made by perforin allow granzymes/granulysin to be internalized

Answer 32

True

Question 33

___ binding to target cell, polymerizing in presence of calcium, and forming transmembrane channels increases the cell permeability and cell death

Answer 33

Perforin

Question 34

True or False: cell death, apoptosis, and increase cell permeability are consequences of granular mediated killing

Answer 34

True

Question 35

In what type of killing does the FasL on the CTL bind to the FAS on target cell?

Answer 35

Fas-FasL Ligand Killing

Question 36

True or False: Fas-FasL Ligand Killing is associated with activation of intracellular caspases in the target cell - which induces apoptosis

Answer 36

True

Question 37

True or False: Natural Killer Cells (CD56+/CD16+) are both T and B cells and require prior sensitization, immunization, or antigen presentation to kill their targets

Answer 37

False - NKC's are neither T or B cells and they do NOT require prior sensitization, immunization, or antigen presentation to kill their targets

Question 38

Another name for NKC? (hint: CD)

Answer 38

CD56+/CD16+

Question 39

True or False: Natural Killer Cells (CD56+/CD16+) are part of the adaptive immune response

Answer 39

False - innate immune response

Question 40

Which cell types have a KIR and recognize LACK of MHC expression?

Answer 40

NK Cells CD56/CD16

Question 41

True or False: Killing by CD56/CD16 cells involves perforins and granzymes

Answer 41

True

Question 42

How can NK Cells be upregulated?

Answer 42

IL-2 and IFN-gamma

Question 43

Macrophages have cell killing mechanisms when stimulated by ___

Answer 43

IFN-gamma

Question 44

Mechanisms (3) of macrophage killing?

Answer 44

-Release lysosomal contents -Release of TNF-a (apoptosis) -Produces N2 and O2 radicals

Question 45

Antibody dependent cellular cytotoxicity (ADCC) is employed by cells that posses __receptor for IgG

Answer 45

Fc

Question 46

Killer (K) Cell in ADCC have a CD16 marker, which means it has a receptor for __ region of __

Answer 46

Fc; IgG

Question 47

True or False: CD16 is a FcgRIII (meaning it binds to heavy chain of of IgG3 subclass)

Answer 47

True