Lab 2

Question 1

Which lens does the user look through on a microscope?

Answer 1

Ocular lens

Question 2

Which lens is used to set various magnifications?

Answer 2

Objective lens

Question 3

Which lens typically is 10x or 15x?

Answer 3

Ocular

Question 4

Which lens has the most varied possible magnification lenses?

Answer 4

Objective

Question 5

Total magnification =

Answer 5

Ocular zoom x Objective zoom

Question 6

If the ocular lens is 10x and the objective lens is set to 4x, what is the total magnification?

Answer 6

40x

Question 7

If the ocular lens is 10x and the objective lens is set to 100x, what is the total magnification?

Answer 7

1000x

Question 8

If the ocular lens is 10x and the objective lens is set to 40x, what is the total magnification?

Answer 8

400x

Question 9

What part of the microscope holds the specimen in place?

Answer 9

Specimen stage and clips

Question 10

What purpose does the stage mover serve on the microscope?

Answer 10

To adjust the stage left/right or forward/backward

Question 11

Why would you use the stage mover?

Answer 11

To center the subject in the field of view

Question 12

How is the nosepiece on the microscope used?

Answer 12

Rotate to choose the desired objective lens

Question 13

What is the light source for a compound microscope?

Answer 13

Light bulb

Question 14

What is the Rheostat used for?

Answer 14

To set a brightness for the bulb

Question 15

What is the Condenser Diaphragm used for?

Answer 15

To control the amount of light entering the objective lens.

Question 16

If you look through the ocular lens and don’t see anything, what items would you check?

Answer 16

• power switch - plug (at outlet and at microscope) - rheostat setting - condenser diaphragm is open - objective lens is locked in place - slide position - if outlet is dead

Question 17

At which settings are you permitted to use both course and fine adjustment knobs?

Answer 17

4x and 10x

Question 18

At which settings are you use only fine adjustment knobs? Why?

Answer 18

40x and 100x. Using the course adjustment could push the objective lens through the slide.

Question 19

After a slide is in place on the stage, what is used to center the image?

Answer 19

The stage mover knobs

Question 20

As you zoom in the microscope, would you need to increase or decrease the amount of light?

Answer 20

Increase

Question 21

As you zoom out the microscope, which you need to increase or decrease the amount of light?

Answer 21

Decrease

Question 22

A microscope’s usefulness depends mainly on what two things?

Answer 22

Magnification and Resolution Power

Question 23

The minimum distance between two points at which those points can still be seen as different objects refers to what microscope feature?

Answer 23

Resolution Power

Question 24

What does resolution power provide?

Answer 24

Greater image detail

Question 25

High zoom + low resolution results in

Answer 25

Narrow field of view with blurry to no detail image

Question 26

Low zoom + high resolution results in

Answer 26

Wide field of view with sharp image

Question 27

High zoom + high resolution results in

Answer 27

Narrow field of view with sharp, detailed image

Question 28

What is seen through the ocular lens is called...

Answer 28

the field of view

Question 29

If the subject is upside down and inverted, what is the problem?

Answer 29

The subject was placed on the stage upside down.

Question 30

T or F: Specimen size is the same as field of view diameter.

Answer 30

False

Question 31

If you move the stage right, the image will move...

Answer 31

Left

Question 32

The distance between the nearest and farthest objects that remain in focus is called...

Answer 32

Depth of field

Question 33

When zoom increases, field of view

Answer 33

Decreases

Question 34

When zoom decreases, field of view

Answer 34

Increases

Question 35

If the specimen has disappeared from the field of view, what has happened?

Answer 35

Zoom has increased

Question 36

Why do we center the image each time the zoom is changed?

Answer 36

To make sure it remains in field of view as zoom settings increase

Question 37

The field of view is measured in

Answer 37

mm

Question 38

To determine the width of the field of view, we measure its

Answer 38

Diameter

Question 39

When we have an original diameter and magnification settings and are provided either one new diameter or magnification setting, what is the formula to figure out the missing diameter or magnification setting?

Answer 39

D₁M₁ = D₂M₂

Question 40

D₁M₁ = D₂M₂ What is the formula to solve for D₂?

Answer 40

D₂ = D₁M₁ / M₂

Question 41

D₁M₁ = D₂M₂ What is the formula to solve for M₂?

Answer 41

M₂ = D₁M₁ / D₂

Question 42

A light microscope that uses multiple lenses for magnification is called a...

Answer 42

Compound microscope

Question 43

What is the field of view?

Answer 43

What the observer sees when they look through the ocular lens.

Question 44

What happens if you use a t-shirt or kleenex to clean the microscope lenses?

Answer 44

It will scratch them