Lab Exam II Microbiology Flashcards Preview

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Flashcards in Lab Exam II Microbiology Deck (36)
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1

Lyses cells

Sodium hydroxide/Sds

2

Precipitates debris

KOAc & detergent

3

Precipitates DNA

90% ethanol

4

Resuspend's DNA

Triss EDTA buffer

5

Chelates calcium and magnesium

EDTA

6

Washes plasmid

70% ethanol

7

Can differentiate between endogenous and DNA that is not from the cell. Spares the bacterium from infection – recognizes palindromic sequences.sticky and allow them to combine with complementary single-stranded DNA. Add last.

Restriction endonucleases

8

nicks are annealed by...

DNA ligase

9

Small colonies that have not taken up the plasmid (White) that form around a large colony that has released beta lactamase which degrades ampicillin and it's vicinity.

Satellite colonies

10

Degrades ampicillin

Beta lactamase

11

Helps us digest carbohydrates and synthesize certain vitamins. We provide E. coli with a nice warm place to live with food. It is also protected from viruses and competition with other bacteria.

E. coli

12

Is inhibiting the growth of one type of organism while permitting the growth of another as with an antibiotic.

Selection

13

When you are making a distinction between different organisms such as in a blue white screening.

Screen

14

Use reverse transcriptase to synthesize cDNA from post transcriptionally modified mRNA. The cDNA can be correctly translated in a prokaryotic cell.

How would you find a gene?

15

Extra chromosomal DNA commonly used as cloning vector. Origin of replication, selectable marker, cloning site into which the gene can be inserted.

Plasmid

16

Isolate DNA. Cleve DNA with restriction endonucleases and splice into a vector. Transform cells. Use selections and screens to elucidate which clones have taken up plasmid. Replica plating. Use of a primer will help you elucidate the clone expressing The gene of interest.

Making a gene library

17

Cleves media having x-gal so bacteria producing the enzyme turn blue.
There must be a recognition sequence in the beta-galactosidase Jean so that a gene of interest may be inserted and interrupt the beta galactosidase gene. Transformants will be white. Plate on antibiotic/X gal media.

Beta galactosidase

18

Bioxidation in which molecular oxygen can serve as the final electron acceptor.

Aerobic

19

Bioxidation in which organic ions other than oxygen such as nitrate or sulfate can serve as the final electron acceptor.

Anaerobic

20

Biooxidative process not requiring oxygen in which an organic substance serves as the final electron acceptor (lactic acid or alcohol fermentation. Yellow/yellow with gas)

Fermentation

21

Alkaline slant (red) and acid butt (yellow) with or without gas production breaks in auger butt.

Triple sugar iron test. Fermentation of glucose.

22

Acid slant yellow and acid butt (yellow) with or without gas production.

Triple sugar iron test. Lactose and or sucrose fermentation.

23

Alkaline slant (red) and alkaline butt (red) or no change (orange/red) butt

Triple sugar iron test. No carbohydrate fermentation has occurred. Peptones are catabolized resulting in an alkaline pH due to the production of ammonia.

24

Medium has sodium thiosulfate and Ferris sulfate to produce blackening.

Triple sugar iron test. hydrogen sulfide production.

25

Positive result produces a cherry red reagent layer.

Indole production test

26

Glucose fermentation reaction with methyl red PH reagent. Positive test is red. Negative test is yellow.

Methyl red test

27

Glucose fermentation. Red layer is a positive test.

Voges-proskauer test

28

Positive tube shows growth on slanted surface

Citrate utilization test

29

Prevents transpeptideation resulting in a weakened peptidoglycan

Penicillin

30

Has an affinity for bacterial ribosomes causing missreading of codons in mRNA interfering with protein synthesis.

Streptomycin