Lab Procedure- Bacterial Transformation

Question 1

What is transformation?

Answer 1

The ability that bacteria cells have to uptake naked DNA.

Question 2

What bacteria species are we transforming in this lab procedure?

Answer 2

We are transforming Escherichia coli.

Question 3

What are we transforming the bacteria cells with, in this lab procedure?

Answer 3

We are transforming the bacteria cells with the pGLO plasmid.

Question 4

What specific gene is known as the “foreign” jellyfish gene?

Answer 4

The GFP (Green Florescent Protein) gene is the “foreign” jellyfish gene.

Question 5

How many plates do we inoculate during the lab procedure?

Answer 5

3? Unsure of answer