Lab Techniques

Question 1

What is the primary purpose of protein isolation?

Answer 1

To separate specific proteins from a complex mixture for analysis or use.

Question 2

True or False: gas chromatography is a technique used to isolate proteins based on their specific interactions with ligands.

Answer 2

false

affinity chromatography

Question 3

Fill in the blank: The technique used to separate proteins based on size is called __________.

Answer 3

gel electrophoresis

Question 4

What is the role of SDS in SDS-PAGE?

Answer 4

To denature proteins and impart a negative charge for separation by size.

Question 5

Which method uses a matrix to separate proteins based on their isoelectric point?

Answer 5

Isoelectric focusing

Question 6

What does the term “purity” refer to in protein isolation?

Answer 6

The extent to which a protein is free from contaminants.

Question 7

True or False: purification can be used to separate proteins based on their density.

Answer 7

false

centrifugation

Question 8

What is the purpose of dialysis in protein purification?

Answer 8

To remove small molecules and salts from a protein solution.

Question 9

What is the main principle behind size exclusion chromatography?

Answer 9

Separation based on the size of molecules, allowing smaller molecules to enter the pores of the matrix.

Question 10

Which technique is commonly used to determine protein concentration?

Answer 10

Bradford assay

Question 11

Fill in the blank: The __________ technique uses antibodies to specifically bind to proteins of interest.

Answer 11

immunoprecipitation

Question 12

What does LC-MS stand for?

Answer 12

Liquid Chromatography-Mass Spectrometry

Question 13

True or False: Western blotting is used to detect specific proteins after gel electrophoresis.

Answer 13

True

Question 14

What is the significance of using a standard curve in protein assays?

Answer 14

To quantify unknown protein concentrations by comparison.

Question 15

What type of chromatography separates proteins based on their charge?

Answer 15

Ion exchange chromatography

Question 16

Fill in the blank: The __________ is a technique that separates proteins based on their thermal stability.

Answer 16

thermal shift assay

Question 17

What is the main advantage of using high-performance liquid chromatography (HPLC)?

Answer 17

Increased resolution and speed in separating proteins.

Question 18

True or False: The native PAGE technique preserves the protein’s native structure.

Answer 18

True

Question 19

What is the main purpose of a protein assay?

Answer 19

To measure the concentration of proteins in a sample.

Question 20

Which technique is best for analyzing protein-protein interactions?

Answer 20

Co-immunoprecipitation

Question 21

What is the role of a loading buffer in SDS-PAGE?

Answer 21

To provide density and color to the sample for loading into the gel.

Question 22

True or False: Mass spectrometry can provide information about the molecular weight of proteins.

Answer 22

True

Question 23

What does the term ‘fractionation’ refer to in protein isolation?

Answer 23

The process of separating proteins into different fractions based on specific properties.

Question 24

What is the key difference between analytical and preparative chromatography?

Answer 24

Analytical chromatography is used for analysis, while preparative chromatography is used for isolating large quantities of protein.

Question 25

Fill in the blank: __________ is a technique that quantifies protein concentration by measuring absorbance at 280 nm.

Answer 25

UV-Vis spectroscopy

Question 26

What is the significance of using a molecular weight marker in gel electrophoresis?

Answer 26

To estimate the size of unknown proteins in the gel.

Question 27

True or False: Protein precipitation is a method to concentrate proteins from a solution.

Answer 27

True

Question 28

What is the main use of a protein ladder in electrophoresis?

Answer 28

To determine the size of proteins by comparison.

Question 29

What does the term 'denaturation' refer to in protein analysis?

Answer 29

The process of altering a protein's natural structure, typically through heat or chemicals.

Question 30

Fill in the blank: The __________ assay measures the enzymatic activity of a protein.

Answer 30

enzyme activity

Question 31

What does SDS in SDS-PAGE stand for?

Answer 31

Sodium Dodecyl Sulfate

Question 32

Which technique would you use to analyze protein-protein interactions without denaturing the proteins?

Answer 32

Native PAGE

Question 33

What is the main purpose of adding SDS to the protein sample in SDS-PAGE?

Answer 33

To impart a negative charge to the proteins and denature them