LEC 6 - Opportunistic Fungal Infections Flashcards Preview

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Flashcards in LEC 6 - Opportunistic Fungal Infections Deck (49):
1

What is an important step in the life cycle of Phyium insidiosum?

Needs to infect plants to complete cycle

2

What allows P. Insidiosum to invade animal tissue?

Motile zoospores w/ germ tube production

3

Affected Animals: P. Insidiosum

Horses
Dogs
Calves
Sheep
Cats

4

Lesions: P. Insidiosum

Granulomatous tissues - Subcutaneous or Intestinal

5

Infective stage: P. Insidiosum

Animal wades in water
Zoospore infects = oppurtunistic

6

How doe the zoospores of P. Insidiosum invade the tissue?

Through the germ tubes that produce aseptate hyphae

7

What does P. Insidiosum do while its disseminating?

Causes thrombosis

8

What causes tissue damage with P. Insidiosum infections?

Degranulating EOS + Mast cells

9

Where do lesions in horses tend to occur with P. Insidiosum infections?

Body parts that contacted the water

10

Disease: P. Insidiosum

Cutaneous pythiosis

11

Lession appearance: P. Insidiosum in Horse

Large, circular granulomatous nodules
Ulcerate
Pruritus
Necrotic yellowish coral-like mass

12

What can happen with chronic P. Insidiosum infections?

Bone involvement

13

Define: Enteric pythioss

Stenotic fibrous GI lesions

14

Target Organs: P. Insidiosum in DOgs

Stomach + SI

15

Clinical Signs: P. Insidiosum in Dogs

Vomiting
Wt. Loss
Diarrhea
PALPABLE ABDOMINAL MASS

16

What is causing the abdominal mass in a dog with a P. Insidiosum infection?

Extension into pancreas + bile ducts + mesenteric LN

17

Where do cutaneous lesions in dogs tend to occur with P. Insidiosum infections?

Base of tail
Extremities
Perineum
Ventral Neck

18

Diagnosis: P. Insidiosum

Tissue stain
Immunofluorescence
PCR
Culture
Serology

19

Culture: P. Insidiosum

Sabouraud dex. agar

20

ID colony: P. Insidiosum

Aseptate hyphae
Colorless to white w/ short aerial hyphae

21

Stain: P. Insidiosum

PAS + Methenamine silver

22

P. Insidiosum: Treatment Dogs

Aggressive surgery
Remove infected bowl
Amputate distal limbs infected
NO ERGOSTEROL IN MEMBRANE - still txt w/ Itraconazole

23

P. Insidiosum: Treament Horses

Wide surgical excision + Immunotherapy

24

Describe: P. Insidiosum Immunotherapy

Regulation of T cells from Th1 to Th2

25

Describe P. Insidiosum Thermotherapy

Apply local heat daily to lesion
Enhances intracellular killing capability of NEU

26

Morphology: Sporothrix schenickii

Dimorphic temperature dependent fungus

27

Etiology: S. Schenickii

Worldwide

28

Culture for Environmental form :S. Schenickii

SDA

29

Colony: S. Schenickii

Hyphae + Condiophores w/ rosette clusters of pear shapped conidia

30

Environmental form: S. Schenickii

25 deg
Hyphae + Condiphores

31

Infective form: S. Schenickii

37 deg
Cigar-shapped pleomorphic budding yeast cells

32

Culture for Infective form: S. Schenickii

Brain Heart Infusion Agar `

33

Clinical Signs: S. Schenickii = Horse/Dogs

Cutaneous/Lymphocytaneous form

34

Clincal Signs: S. Schenickii = Cats

Lymphocutaneous
Disseminated

35

Describe: Equine Sporotrichosis

Lymphocutaneous
Spores enter skin abrasion
Nodules develope along superficial lymphatic vessesl

36

Describe Feline Sporotrichosis

Primary nodular lesions
Ulcerative with seropurulent exudate
Extends to lymphatics

37

Areas of infection: S. Schenickii - Felines

Limb extermities
Head
Tail

38

Area most infected with S. Schenickii in cats

Brazial

39

Describe Canine Sporotrichosis

Multiple cutaneous lesions
Ulcerated + Crusted + Alopecic
Occasional lymphocutaneous involvement

40

Treatement: S. Schenickii Horse

Systemic Isodid preparation
Organic perferred (EDDI)
Feed additive

41

Treatment: S. Schenickii - Dogs/Cats

Itraconazole

42

Infection form: P. Carinii

Asexual trophozoite

43

Reproducing form: P. Carinii

Sexual reproducing cyst

44

Predisposition: P. Carinii

High prevalence of young animals
Airborne/transplacental

45

Animals infected: P. Carinii

Dogs
Horses
Rabbits
Cats
Pigs

46

DOC: P. Carinii

Trimethoprim
-- or --
TMS
Supportive care

47

Culture: P. Carinii

CAN NOT BE

48

PCR: P. Carinii where from?

lung tissue

49

Clincal signs: P. Carinii

Diffuse Pulmonary consolidation
Marked thickening alveolar space
Proteinaceous exudate in alveoli