Flashcards in Lecture 4 Deck (16):
H & E Eosin Stain
Stains acidophilic (basic) structures pink. Cytoplasm, mitochondria, SER, Filaments.
Stains basophilic (Acidic) structures purple. Nucleus, heterochromatin, nucleolus, RER, euchromatin not well stained.
Acid and Schiff Reagent, stains nuclear DNA a magenta color; Can see DNA in different mitosis phases
Periodic-Acid-Schiff (PAS) reaction
Stains carbohydrate rich macromolecules a deep red. Mucus, glycosaminoglycans (GAGs), glycoproteins, glycogen. Can see goblet cells, glycocalyx of microvilli, heparan (contains GAGs) of basement membrane, Liver
Cell components are stained a different color from that of the dye itself. Ex: toluidine blue
Dye is a dark blue, haparin in the mast cells stains magenta with toluidine blue.
Stains fat (mostly triglycerides) in adipocytes a red color. Obese individuals with type II diabetes suffer from fatty liver, can be visualized with this stain
Lipid stain a blackish color, stains myelin of axons, lecithin, sphingomyelin
Histology of ECM
Elastic fibers and collegen fibers are acidophilic stain pink with eosin
Elastic fibers stained black, found in ECM and in other connective tissue
Mallory Trichrome stain
Collagen fibers are stained blue, types I and III. Used in clinic to diagnose diseases with fibrosis (hepatic, pulmonary)
Type III collagen (reticular fibers) stains
Azo Dye method
Stains acid phosphotase red, which is localized to lysosomes
Succinate Dehydrogenase (SDH) tetrazolium method
Localization of succinate dehydrogenase to mitochondria
How does immunihistochemistry work
there are antigens are in the tissue, and there are antibodies (with fluorescent or peroxidase attached) that are bonded to the antigens.