Lecture 4 Flashcards Preview

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Flashcards in Lecture 4 Deck (16):
1

H & E Eosin Stain

Stains acidophilic (basic) structures pink. Cytoplasm, mitochondria, SER, Filaments.

2

Hematoxylin

Stains basophilic (Acidic) structures purple. Nucleus, heterochromatin, nucleolus, RER, euchromatin not well stained.

3

Feulgen Reaction

Acid and Schiff Reagent, stains nuclear DNA a magenta color; Can see DNA in different mitosis phases

4

Periodic-Acid-Schiff (PAS) reaction

Stains carbohydrate rich macromolecules a deep red. Mucus, glycosaminoglycans (GAGs), glycoproteins, glycogen. Can see goblet cells, glycocalyx of microvilli, heparan (contains GAGs) of basement membrane, Liver

5

Metachromasia

Cell components are stained a different color from that of the dye itself. Ex: toluidine blue

6

Toluidine Blue

Dye is a dark blue, haparin in the mast cells stains magenta with toluidine blue.

7

Sudan IV

Stains fat (mostly triglycerides) in adipocytes a red color. Obese individuals with type II diabetes suffer from fatty liver, can be visualized with this stain

8

Osmium Tetraoxide

Lipid stain a blackish color, stains myelin of axons, lecithin, sphingomyelin

9

Histology of ECM

Elastic fibers and collegen fibers are acidophilic stain pink with eosin

10

Resorcin-fuschin stain

Elastic fibers stained black, found in ECM and in other connective tissue

11

Mallory Trichrome stain

Collagen fibers are stained blue, types I and III. Used in clinic to diagnose diseases with fibrosis (hepatic, pulmonary)

12

Silver Stain

Type III collagen (reticular fibers) stains

13

Azo Dye method

Stains acid phosphotase red, which is localized to lysosomes

14

Succinate Dehydrogenase (SDH) tetrazolium method

Localization of succinate dehydrogenase to mitochondria

15

How does immunihistochemistry work

there are antigens are in the tissue, and there are antibodies (with fluorescent or peroxidase attached) that are bonded to the antigens.

16

Peroxidase activity demonstration

Diaminobenzidine (DAB) reactes with peroxidase to create insoluble brown color compound.