Lecture 9 Flashcards
(19 cards)
Once pyruvate is produced, where is it transported?
Mitochondrion
The Krebs cycle is part of…
Substrate level phosphorylation
What is the first step of the oxidation of pyruvate?
Decarboxylation: COO- group forms CO2
The oxidation of pyruvate is carried out by…
pyruvate dehydrogenase enzyme complex
What is the second step of the oxidation of pyruvate?
reduction: NAD+ —> NADH
What is the third step?
the remaining acetyl group is transferred to the molecule coenzyme A
Through which bond is the acetyl group transferred to the coenzyme A?
acetyl thioester bond
Energy is required for the third step, where does it come from?
the redox reaction in the second step
What does the oxidation of pyruvate yields?
1 co2, 1 NADH, 1 acetyl CoA molecule
Where does the Krebs cycle takes place?
mitochondrial matrix
Step 1 of Krebs cycle…
Condensation: oxaloacetate + acetyl group form citrate
Step 2-3 of Krebs cycle…
Isomerization: citrate is transformed into isocitrate by removing and adding water
step 4 of Krebs cycle…
first oxidation: NAD+»_space;> NADH
Step 5 of Krebs cycle…
second oxidation NAD+»_space;> NADH
Step 6 of Krebs cycle…
substrate level phosphorylation: GTP can transfer a phosphate to ADP
Step 7 of Krebs…
Third oxidation FAD»_space;> FADH2
What does the Krebs cycle yields?
1 ATP, 2 CO2, 3 NADH, 1 FADH2
What is yield for 1 glucose?
2 FADH2, 10 NADH, 4 ATP BUT 30 ATP AFTER CHEMIOSMOSIS
What is chemiosmosis?
The energy released by the transport allows for the formation of ATP from ADP and inorganic phosphate
