Flashcards in Lecture 22 Deck (19):
What is DNA cloning?
it is the process where well-defined DNA segments are prepared. These can be propagated in multiple identical copies.
The cloning for pieces of DNA uses...
bacteria and their plasmids
What are plasmids?
small circular DNA molecules that replicate separately from bacterial chromosome
For what is gene cloning useful?
to make copies of particular gene and for producing protein product
how does it work?
the foreign DNA is inserted into a plasmid and the recombinant plasmid is inserted into the bacterial cell.
know the plasmid components
what is transformation?
the process by which another organism takes the DNA of another organism
In gene cloning what is called the original plasmid?
What is the use of PCR in DNA cloning?
PCR produces multiple copies of the target DNA
What is the use of restriction enzymes in DNA cloning?
the dna is added to the restriction sites
DNA sequencing uses...
complementary base pairing
what was the first automated method to be employed?
dideoxy chain termination method
How does dideoxy chain termination works?
the modified nucleotides (dideoxy-ribonucleotides) attach to the synthesized DNA strands. Each type of ddNTP is tagged with distinct fluorescent label that identifies nucleotide at end of each DNA fragment.
know about the next-generation sequencing
To overcome differences in promoters, scientists use an__________ a cloning vector containing highly active bacterial promoter
To avoid eukaryote-baterial incompatibility issues, ________ is used, such as ____ for cloning and expressing genes
eukaryotic cells, yeast
cultured _________ or ______ cells may be used to express and study proteins
What is he method used to introduce recombinant DNA to eukaryotic cells?