Lecture 14 Flashcards Preview

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Flashcards in Lecture 14 Deck (18):
1

Where are genes found?

chromosomes

2

Genetic information is located on...

DNA

3

DNA replicates into which fashion?

semi-conservative

4

What did Frederick Griffith discover?

Traits can be transferred between different bacterial strains

5

What is the experiment of Frederick Griffith?

he injected a group of mice with live S strain (pathogenic bacteria), the mice died.
he injected of living R (nonpathogenic)... mice survived
he injected killed S (nonpathogenic)...mice survived
he injected living R strain with dead S strain... mice died

6

What do bacteria exchange?

plasmids

7

How much monomers do proteins have? How about DNA?

20 and 4

8

What was the experiment of Avery, MacLoed and McCarthy?

They purified cell extracts (proteins, DNA, RNA, carbohydrates, lipids) from dead S strain bacteria. They incubated the extracts with R strain cells. The strain that carried the DNA transferred the S characteristic.

9

What was showed with the experiment of Avery, Macloed and McCathy?

DNA is the transforming factor

10

What atoms is found in proteins but it is not found in DNA?

sulphur

11

What atoms is found on the DNA but not in proteins?

phosphorous

12

In a virus where is DNA found?

inside the head, the rest is proteins

13

What is the experiment of Hershey and Chase?

They put two different bacteriophages, one with radioactive S36 and one with P32. You inject bacteria with each bacteriophage, then they are blended to remove the viruses. This is centrifuge and it gives a supernatant (virus proteins) and pellet (bacterial cell contents) is heavier than protein.

14

What is the experiment of Chargaff?

He compared the DNA of various species using paper chromatography.

15

What did it showed?

A-T,G-C

16

Who came up with a double helix structure?

Watson and Crick

17

Know the different DNA replication

semi-conservative, conservative and dispersive

18

Who found that DNA was semi-conservative?

Meselon and Stahl