Lesson 12: Culture Media

Question 1

are cultured in either liquid (broth) or on solid (agar) artificial media

Answer 1

bacteria and fungi

Question 2

pioneered the use of agar as a base for culture media by Fannie eilshemius

Answer 2

robert koch

Question 3

developed the petri dish (plate)

Answer 3

Richard petri

Question 4

a container for solid culture media

Answer 4

petri dish (plate)

Question 5

developed the pour plate method and was the first to use solid culture media for culture of bacteria

Answer 5

Koch

Question 6

a nutrient material prepared for the growth of microorganisms in a laboratory

Answer 6

culture medium

Question 7

when microbes are introduced into a culture medium to initiate growth growth

Answer 7

inoculum

Question 8

microbes that grow and multiply in or on a culture medium

Answer 8

culture

Question 9

process of growing microorganisms in culture by taking bacteria from the infection site

Answer 9

cultivation

Question 10

a laboratory culture containing a single species of organism

Answer 10

pure culture

Question 11

bacterial cultivation has three main purposes;

Answer 11

1. to grow and isolate all bacteria
2. infection and contaminants or colonizers
3. identification and characterization

Question 12

Ingredients of culture media. components of culture media

Answer 12

1. water
2. agar
3. peptone
4. yeast extract
5. malt extract
6. blood and serum

Question 13

phases of growth medua

Answer 13

1. liquid (broth) media
2. solid (agar) media
3. semisolid media

Question 14

liquid (broth) media

Answer 14

• media nutrients are dissolved in water

Question 15

solid (agar) media

Answer 15

-adding a solidifying agent to the nutrients and water

Question 16

most common solidifying agent

Answer 16

agarose

Question 17

petri dish containing the agar

Answer 17

agar

Question 18

clones of cella originating from a single bacterial cell

Answer 18

colonies

Question 19

a single colony or clone are considered “pure” nutrient agar

Answer 19

bacterial cultures

Question 20

simplest and most common medium used routinely in diagnostic laboratories

Answer 20

2% agar to nutrient broth

Question 21

other example of solid media

Answer 21

blood agar, chocolate agar, macConkey agar

Question 22

semisolid media

Answer 22

-where agar is added to media in concentrations that are too low to solidify

Question 23

is an example of a simple liquid medium that consist peptone, meat extract sodium chloride and water

Answer 23

nutrient broth

Question 24

example of a simple solid medium

Answer 24

nutrient agar

Question 25

a media that contain some ingredients of unknown chemical composition

Answer 25

complex media

Question 26

the complex media contain;

Answer 26

water, carbon source, various salts, source of amino acids and nitrogen

Question 27

special media

Answer 27

1. enriched media 2. enrichment media 3. selective media 4. indicator media 5. transport media 6. sugar media

Question 28

enriched media

Answer 28

solid media that facilitate growth

Question 29

enriched media

Answer 29

solid media that facilitate growth

Question 30

enrichment media

Answer 30

are liquid media that stimulate the growth of certain bacteria

Question 31

selective media

Answer 31

are solid that contain substances that inhibit the growth of all but a few bacteria

Question 32

differential or indicator media

Answer 32

distinguish one microorganisms from another growing on the same media

Question 33

transport media

Answer 33

used to maintain the viability of certain delicate organisms in clinical specimens

Question 34

sugar media

Answer 34

contains 1% "sugar"

Question 35

sugar media examples used for fermentation test

Answer 35

glucose, sucrose, lactose and mannitol

Question 36

preserving bacterial cultures

Answer 36

refrigeration deep freezing lyophilization (freeze drying) cold storage drying methods

Question 37

can be used for short term storage of bacterial cultures

Answer 37

refrigeration

Question 38

is a process in which a pure culture of microbes is placed in a suspending liquid and quick frozen

Answer 38

deep freezing

Question 39

a suspension of microbes is quickly frozen

Answer 39

lyophilization

Question 40

common method of preserving stains of bacteria

Answer 40

cold storage

Question 41

drying suspensions of bacteria for preservation purposes have been developed

Answer 41

drying methods

Question 42

sterilizing agent with very high penetrating power

Answer 42

radiations

Question 43

remove contaminating Microorganisms from solution

Answer 43

filtration

Question 44

use of high-frequency sound waves beyond the sensitivity of the human ear

Answer 44

sonication

Question 45

most dependable method of sterilization

Answer 45

heat

Question 46

natural.method in sterilization of water in tanks

Answer 46

sunlight

Question 47

process of inactivating microorganisms by direct exposure to chemical and physical agents

Answer 47

disinfection

Question 48

are products that destroy or inhibit the growth of microy

Answer 48

disinfectants

Question 49

products that destroy or inhibit the growth of microorganisms in or on living tissue

Answer 49

antiseptics

Question 50

types of disinfectants

Answer 50

phenolic compounds Halogens alcohols aldehydes gases surface active agents oxidizing agent dyes heavy metals acids alkalis