Lesson 9 Part 2

Question 1

The proliferation or multiplication of becateria

Answer 1

Bacterial growth

Question 2

Bacteria multiply by _____

Answer 2

Binary fission

Question 3

The time it takes a particular bacterial species to undergo binary fission

Answer 3

Generation time

Question 4

Bacteria with short generation time

Answer 4

Rapid growers

Question 5

Bacteria with long generation time

Answer 5

Slow growers

Question 6

Microorganisms that are difficult to grow in the laboratory

Answer 6

Fastidious

Question 7

The media that are used in microbiology laboratories to culture bacteria are called

Answer 7

Artificial media or synthetic media

Question 8

All the ingredients are known; the medium was prepared in the laboratory

Answer 8

Chemically defined medium

Question 9

The exact contents are not known; contain ground up or digested extracts from animal organs and plants

Answer 9

Complex medium

Question 10

They are also known as broth

Answer 10

Liquid media

Question 11

Contained in tubes and are thus often referred to as tube media

Answer 11

Liquid media

Question 12

Prepared by adding agar to liquid media and then pouring the media into tubes or petrid dish

Answer 12

Solid media

Question 13

Complex polysaccharide that is obtained from red marine algae

Answer 13

Agar

Question 14

A broth or solid medium containing a rich supply of special nutrients

Answer 14

Enriched medium

Question 15

Used to discourage the growth of certain organism

Answer 15

Selective medium

Question 16

Inhibits growth of gram POSITIVE bacteria

Answer 16

MacConkey agar

Question 17

Inhibits growth of gram negstivr

Answer 17

Phenylethyl alcohol (PEA) and Colistinnalidixic acid (CNA)

Question 18

Inhibits growth of gram negative

Answer 18

Phenylethyl alcohol agar (PEA) and collistinnalidixic acid (CNA)

Question 19

Chocolate agars; selective for N. Gonorrheae

Answer 19

Thayer-Martin agar and Martin Lewis agar

Question 20

Grows only salt tolerant (haloduric) bacteria

Answer 20

Mannitol salt agar (MSA)

Question 21

Allows one to readily differentiate among the various types of organisms that are growing on the medium

Answer 21

Differentiate Medium

Question 22

It differentiate between lactose-fermenting and non-lactose fermenting gram negative bacteria

Answer 22

MacConkey Agar

Question 23

It used to screen s aureus

Answer 23

Mannitol Salt Agar

Question 24

Ueed to determine the type of hemolysis

Answer 24

Blood agar

Question 25

A very popular liquid medium for use in the bacteriology laboratory

Answer 25

Thioglycollate broth (THIO)

Question 26

It is practised in the microbiology laboratory to prevent infection of individuals and contamination of the work environment, clinical specimens, and culture

Answer 26

Aseptic Technique

Question 27

After media is inoculated, the next step is

Answer 27

Incubation

Question 28

It is placed in a chamber that contains the appropriate atmosphere and moisture level and is set to maintain the appropriate temperature

Answer 28

Incubation

Question 29

To culture most human pathogens, the incubator is set at ______ to _____ C

Answer 29

35°C to 37°C

Question 30

What are the three types of incubators

Answer 30

CO2 incubators Non-CO2 incubators Anaerobic incubators

Question 31

Involves the destruction or elimination of all microbes, including cells, spores, and viruses

Answer 31

Sterilization

Question 32

Describes the elimination of most or all pathogens (except bacterial spores) from non living objects

Answer 32

Disinfection

Question 33

A method of disinfecting liquids; used to eliminate pathogens from milk and most other beverages

Answer 33

Pasteurization

Question 34

Chemicals used to disinfect inanimate objects, such as bedside equipment and operating rooms

Answer 34

Disinfectants

Question 35

Solutions used to disinfect skin and other living tissues

Answer 35

Antiseptic

Question 36

The reduction of microbial populations to levels considered safe by public health standards, such as restaurants

Answer 36

Sanitization

Question 37

-cide or -cidal means

Answer 37

killing

Question 38

Example of disinfectants or antiseptics to kill microbes

Answer 38

Germicides Biocides Microbisides

Question 39

They specifically kill bacteria

Answer 39

Bactericides / bactericidal agents

Question 40

Required to kill bacterial endospores

Answer 40

Sporicidal agent

Question 41

Kill fungi, including fungal spores

Answer 41

Fungicidal agents

Question 42

Are used to kill algae in swimming pools and hot tubs

Answer 42

Algicidal agents

Question 43

Destroy viruses

Answer 43

Virucidal agents

Question 44

Kill pseudomonas species

Answer 44

Pseudomonicidal agents

Question 45

Kill M. Tuberculosis

Answer 45

Tuberculocidal Agents