micro lab quiz #3 Flashcards

1
Q

what do you use or antimicrobial susceptibility testing

A

disk diffusion method

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2
Q

when did bacteria start to become resistant to antibiotics

A

1950

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3
Q

who started using disk diffusion method

A

Kirby and bauer in 1966

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4
Q

how long are the filet paper with antibiotics

A

6mm

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5
Q

what are the 6mm paper discs impreganated with

A

standard concentration of antibiotic

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6
Q

what agar is used for standard specifications in diffusion disc method

A

Mueller-Hinton agar plate

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7
Q

what is used to have a standard suspension of organism

A

McFlarland standard

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8
Q

how does disc diffusion work

A

the rate of diffusion through agar is dependent on diffusion of solubility of the drug on the mueller-winton agar

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9
Q

what is diffusion heavily ties to

A

molecular weight

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10
Q

antibiotic

A

chemicals that have select toxicity

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11
Q

broad spectrum

A

works against GNR AND GPR

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12
Q

narrow spectrum

A

work against GNR or GPR not both

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13
Q

narrow spectrum is used if possible

A

doesn’t get as much microbiome

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14
Q

disc diffusion rules

A

the antibiotic can only kill if it touches

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15
Q

tiny mcg

A

can reach super far in 12 hours

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16
Q

large mcg

A

can’t reach as far as tiny mcg

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17
Q

difference between tiny mcg and large mcg is

A

spreading rate of microorganism

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18
Q

bacteriostatic

A

stop production and allows your immune system to catch up

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19
Q

bactericidal

A

antibiotic kills bacteria * more side effects

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20
Q

zone of inhibition are measured bu

A

mm

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21
Q

is endospores kills in pasteurization

A

no

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22
Q

pasteurization

A

super high heat then rapid cooling

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23
Q

what does pasteurization kill

A

most pathogens

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24
Q

component of milk

A

lactose, proteins(casin), water

25
Q

what makes milk go bad

A

Casin curdles which means ph dropped due to the lactic acid build up

26
Q

lipid in milk

A

3.5% fat whole

27
Q

are enteric found in milk

A

no

28
Q

bacteria microbiome

A

lactococci, lactobacillus, enteroccus

29
Q

what strains of bacteria are in yogurt

A

streptococcus and lactobacillus

30
Q

yogurt

A

bacteria feed of each others acidic waste

31
Q

what is the carbon source in sabdex-saboural dextrose agar

A

glucose

32
Q

what pathogens is pasteurization trying to get rid of

A

E. coli and salmonella

33
Q

what does the low ph in the sabdex agar do

A

facilitates isolation of fungi 7 growth at room temperatures

34
Q

what is both favorable for molds and inhibiting bacteria

A

low ph and a lot of sugar

35
Q

mold

A

type of fungi

36
Q

fungi

A

soil microbe

37
Q

what wont work on fungi

A

antibiotics

38
Q

study of fungi

A

clinical mycology

39
Q

what are some examples of fungal infections

A

athletes food, thrush , valley fever

40
Q

about 1.5 mil fungi species

A

only 200 known to cause disease for animals and humans

41
Q

what can lead of fungal meningitis

A

valley fever

42
Q

root of mold

A

rhizoids (anchor below surface

43
Q

what do rhizoids grow out too

A

sporangiospore(stem)

44
Q

what holds the sporangiospores

A

sporangia

45
Q

what needs air movement to spread

A

sporangiospores

46
Q

where did penicillin come from

A

ubiquitous mold called penicillium

47
Q

what did penicillium look like on agar

A

orange

48
Q

white spread in soil

A

penicillium, aspergillus, rhizopus

49
Q

what is found in immunocompromised hosts like aids, leukemia, organ transplant patients

A

aspergillus

50
Q

black bread mold

A

rhizopus

51
Q

what is an opportunist

A

rhizopus

52
Q

why does rhizomes attack strawberries

A

because of low ph

53
Q

what is causative agent of vaginal and utis thrush

A

candida albicans

54
Q

what sends out filaments to spread

A

Candida albicans

55
Q

what forms bio film

A

Candida albicans

56
Q

causative garnet for valley fever

A

coccidiosis immitis

57
Q

60% of people who are exposed to the fungus show

A

no symptoms but will have antibodies positive

58
Q

what stores up valley fever spores

A

dust storm

59
Q

in the soil the fungus grows like

A

mold