micro lab quiz #3

Question 1

what do you use or antimicrobial susceptibility testing

Answer 1

disk diffusion method

Question 2

when did bacteria start to become resistant to antibiotics

Answer 2

1950

Question 3

who started using disk diffusion method

Answer 3

Kirby and bauer in 1966

Question 4

how long are the filet paper with antibiotics

Answer 4

6mm

Question 5

what are the 6mm paper discs impreganated with

Answer 5

standard concentration of antibiotic

Question 6

what agar is used for standard specifications in diffusion disc method

Answer 6

Mueller-Hinton agar plate

Question 7

what is used to have a standard suspension of organism

Answer 7

McFlarland standard

Question 8

how does disc diffusion work

Answer 8

the rate of diffusion through agar is dependent on diffusion of solubility of the drug on the mueller-winton agar

Question 9

what is diffusion heavily ties to

Answer 9

molecular weight

Question 10

antibiotic

Answer 10

chemicals that have select toxicity

Question 11

broad spectrum

Answer 11

works against GNR AND GPR

Question 12

narrow spectrum

Answer 12

work against GNR or GPR not both

Question 13

narrow spectrum is used if possible

Answer 13

doesn’t get as much microbiome

Question 14

disc diffusion rules

Answer 14

the antibiotic can only kill if it touches

Question 15

tiny mcg

Answer 15

can reach super far in 12 hours

Question 16

large mcg

Answer 16

can’t reach as far as tiny mcg

Question 17

difference between tiny mcg and large mcg is

Answer 17

spreading rate of microorganism

Question 18

bacteriostatic

Answer 18

stop production and allows your immune system to catch up

Question 19

bactericidal

Answer 19

antibiotic kills bacteria * more side effects

Question 20

zone of inhibition are measured bu

Answer 20

mm

Question 21

is endospores kills in pasteurization

Answer 21

no

Question 22

pasteurization

Answer 22

super high heat then rapid cooling

Question 23

what does pasteurization kill

Answer 23

most pathogens

Question 24

component of milk

Answer 24

lactose, proteins(casin), water

Question 25

what makes milk go bad

Answer 25

Casin curdles which means ph dropped due to the lactic acid build up

Question 26

lipid in milk

Answer 26

3.5% fat whole

Question 27

are enteric found in milk

Answer 27

no

Question 28

bacteria microbiome

Answer 28

lactococci, lactobacillus, enteroccus

Question 29

what strains of bacteria are in yogurt

Answer 29

streptococcus and lactobacillus

Question 30

yogurt

Answer 30

bacteria feed of each others acidic waste

Question 31

what is the carbon source in sabdex-saboural dextrose agar

Answer 31

glucose

Question 32

what pathogens is pasteurization trying to get rid of

Answer 32

E. coli and salmonella

Question 33

what does the low ph in the sabdex agar do

Answer 33

facilitates isolation of fungi 7 growth at room temperatures

Question 34

what is both favorable for molds and inhibiting bacteria

Answer 34

low ph and a lot of sugar

Question 35

mold

Answer 35

type of fungi

Question 36

fungi

Answer 36

soil microbe

Question 37

what wont work on fungi

Answer 37

antibiotics

Question 38

study of fungi

Answer 38

clinical mycology

Question 39

what are some examples of fungal infections

Answer 39

athletes food, thrush , valley fever

Question 40

about 1.5 mil fungi species

Answer 40

only 200 known to cause disease for animals and humans

Question 41

what can lead of fungal meningitis

Answer 41

valley fever

Question 42

root of mold

Answer 42

rhizoids (anchor below surface

Question 43

what do rhizoids grow out too

Answer 43

sporangiospore(stem)

Question 44

what holds the sporangiospores

Answer 44

sporangia

Question 45

what needs air movement to spread

Answer 45

sporangiospores

Question 46

where did penicillin come from

Answer 46

ubiquitous mold called penicillium

Question 47

what did penicillium look like on agar

Answer 47

orange

Question 48

white spread in soil

Answer 48

penicillium, aspergillus, rhizopus

Question 49

what is found in immunocompromised hosts like aids, leukemia, organ transplant patients

Answer 49

aspergillus

Question 50

black bread mold

Answer 50

rhizopus

Question 51

what is an opportunist

Answer 51

rhizopus

Question 52

why does rhizomes attack strawberries

Answer 52

because of low ph

Question 53

what is causative agent of vaginal and utis thrush

Answer 53

candida albicans

Question 54

what sends out filaments to spread

Answer 54

Candida albicans

Question 55

what forms bio film

Answer 55

Candida albicans

Question 56

causative garnet for valley fever

Answer 56

coccidiosis immitis

Question 57

60% of people who are exposed to the fungus show

Answer 57

no symptoms but will have antibodies positive

Question 58

what stores up valley fever spores

Answer 58

dust storm

Question 59

in the soil the fungus grows like

Answer 59

mold