Flashcards in Microbiology Lab Final Deck (40):
What are the Gram Positive Bacteria Used during lab?
What are the Gram Negative Bacteria Used during lab?
Causes endophthalmitis, right sided endocarditis, and meningitis in comporomised patients.
Part of the normal flora of the human intestine; some strains cause diarrheal disease or urinary tract infections.
Part of the normal microbiota of human skin. Causative agent of skin and wound infections, internal infections, toxic shock syndrome, scaled skin syndrome, and food poisoning.
Found in nasty water. Causes digestion issues and diarrhea.
Causes Malaria. Transmitted by anopheles mosquitos.
Causes toxoplasmosis in humans. Primary host for parasite is domestic cats.
Causes abscess in liver.
Common liver fluke
Rod shaped bacteria
Curved rod bacteria
Corkscrew shaped bacteria
Grape like clusters
What are the general growth mediums used in the lab?
-TSB (trypticase soy broth)
-TSA (trypticase soy agar)
What are the 4 steps in a gram stain?
What is the function of crystal violet in a gram stain?
What is the function of Iodine in a gram stain?
Mordant, fixes purple color in place
What is the function of decolorizer in a gram stain?
Pulls purple from sample. (Too much can cause a gram positive to appear gram negative)
What is the function of safranin in a gram stain?
Works as a counter stain
What is the difference in a gram positive and gram negative cell wall?
Gram negative has a thin layer of peptidoglycan while gram positive has a thick layer of peptidoglycan.
What can you tell by just looking at a bacteria through a microscope?
-Gram positive or negative.
An organism, such as a bacterium, that can live only in the absence of oxygen.
An organism, such as a bacterium, that can live only in the presence of oxygen.
surviving and growing in small amounts of air
An organism, such as a bacterium, that can live in the absence as well as in the presence of atmospheric oxygen.
What is the difference in Eukaryotic and Prokaryotic organisms?
Eukarytoic is US (humans). Have membrane bound organelles.
Prokayrotic do not have membrane bound organelles.
Ribosomes exsist in both. 80s ribosomes in eukarytoes and 70s in prokarytoes.
What is a protein synthesis inhibitor?
Prevents the cross linking of peptidoglycan?
DNA synthesis inhibitors?
What are 3 methods for counting bacteria?
-Most probable number
What is the magnification of a microscope that is using a 10x lens with a 30x lens.
PCR (Polymerase chain reaction)