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Flashcards in Microbiology Lab Final Deck (40):
1

What are the Gram Positive Bacteria Used during lab?

-S. aureus
-S. epidermidis
-M. luteus
-E. faecalis

2

What are the Gram Negative Bacteria Used during lab?

-B. subtilis
-P. aeruginosa
-E. aerogenes
-E. coli
-K. pneumoniae

3

Causes endophthalmitis, right sided endocarditis, and meningitis in comporomised patients.

B. subtilis

4

Part of the normal flora of the human intestine; some strains cause diarrheal disease or urinary tract infections.

E. coli

5

Part of the normal microbiota of human skin. Causative agent of skin and wound infections, internal infections, toxic shock syndrome, scaled skin syndrome, and food poisoning.

S. aureus

6

Found in nasty water. Causes digestion issues and diarrhea.

Giardia lamblia

7

Causes Malaria. Transmitted by anopheles mosquitos.

Plasmodium

8

Causes toxoplasmosis in humans. Primary host for parasite is domestic cats.

Toxoplasma gondii

9

Causes abscess in liver.

Entamoeba histolytica

10

Tape worm

Taenia saginata

11

Common liver fluke

Fasciola hepatica

12

Pin worm

Enterubis vermicularis

13

Spherical bacteria

Cocci

14

Rod shaped bacteria

Bacilli

15

Curved rod bacteria

Vibrios

16

Corkscrew shaped bacteria

Spirilla

17

Flexible helices.

Spirochetes

18

Pairs

Diplococci/diplobacilli

19

Chains

Streptococci/Streptobacilli

20

Grape like clusters

Staphlyococci/staphlybacilli

21

What are the general growth mediums used in the lab?

-TSB (trypticase soy broth)
-TSA (trypticase soy agar)

22

What are the 4 steps in a gram stain?

-Crystal Violet
-Iodine
-Decolorizer
-Safranin

23

What is the function of crystal violet in a gram stain?

Stains peptidoglycan

24

What is the function of Iodine in a gram stain?

Mordant, fixes purple color in place

25

What is the function of decolorizer in a gram stain?

Pulls purple from sample. (Too much can cause a gram positive to appear gram negative)

26

What is the function of safranin in a gram stain?

Works as a counter stain

27

What is the difference in a gram positive and gram negative cell wall?

Gram negative has a thin layer of peptidoglycan while gram positive has a thick layer of peptidoglycan.

28

What can you tell by just looking at a bacteria through a microscope?

-Gram positive or negative.
-Morphology
-Colony

29

An organism, such as a bacterium, that can live only in the absence of oxygen.

Obligate anaerobe

30

An organism, such as a bacterium, that can live only in the presence of oxygen.

Obligate aerobe

31

surviving and growing in small amounts of air

Aerotolerant

32

An organism, such as a bacterium, that can live in the absence as well as in the presence of atmospheric oxygen.

Facultative anaerobe

33

What is the difference in Eukaryotic and Prokaryotic organisms?

Eukarytoic is US (humans). Have membrane bound organelles.
Prokayrotic do not have membrane bound organelles.
Ribosomes exsist in both. 80s ribosomes in eukarytoes and 70s in prokarytoes.

34

What is a protein synthesis inhibitor?

Azithromycin (Z-pak)

35

Prevents the cross linking of peptidoglycan?

Penicillins

36

DNA synthesis inhibitors?

Cipro

37

What are 3 methods for counting bacteria?

-Filtration
-Serial dilution
-Most probable number

38

What is the magnification of a microscope that is using a 10x lens with a 30x lens.

300x

39

Amplifies DNA

PCR (Polymerase chain reaction)

40

What are the 3 parts of a virus?

-Protein capsid
-Nucleic acid
-Glycoprotein