Molecular and Biology Techniques Flashcards Preview

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Flashcards in Molecular and Biology Techniques Deck (13):
1

ELISA Testing

Uses antigen-antibody interactions to determine if desired substance is present. Also enzyme linked included

2

Radioimmunoassay

Use radio labeled antibodies rather than enzyme linked antibodies

3

What result from ELISA testing tells if protein is present

A color change

4

Results of Radioimmunoassay

Radioactivity is measured and competition for antibodies takes place between antigen. Standard curve is formed based on radioactivity

5

Electrophoresis

Separates proteins by size. Electric current applied and samples migrate to positive pole according to size

6

Which molecules travel faster in electrophoresis

Smaller negative molecules

7

Blotting

transfer of DNA or proteins from an electrophoresis gel to a nitrocellulose of membrane to detect nucleic acid or protein

8

Southern Blotting

allows one to detect the presence of specific sequences within a heterogeneous sample of DNA. Isolate and purify target sequences of DNA

9

Northern Blotting

RNA is separated instead of DNA

10

What does Northern Blotting allow one to determine

if specific gene products are being expressed

11

What is Western Blotting used for

detect presence of certain proteins within a sample

12

How does western blotting differ from others

antibodies are used instead of nucleic acids

13

Eastern blotting

used to analyze post translational modifications of peptides