More On Enzymes

Question 1

Enzyme practical :

Answer 1

1. Drop of iodine onto every spotting tile
2. Set up a Bunsen burner and mat , tripod and gauze, put water above until it’s 35°C. Keep this constant
3. Add 3cm cubed of amylase solution and 1cm cubed of buffer solution to a boiling tube. Put them into the water and wait 5 mins
4. Add 3cm cubed of starch solution in a new syringe
5. Mix and start a stop clock
6. Time how long it’s takes for it to turn browns orange . Make sure to time every 10s and add a drop into the wells of solution that’s mixed.
7. Repeat to see how pH afffects the time taken for starch to be broken down.
8. Control all variables.

Question 2

How to calculate the rate of reaction?

Answer 2

Rate = 1000/time