Flashcards in Nitrogen 1 Deck (18)
Which enzyme do nitrogen fixing bacteria use?
Nitrogenase - inhibited by oxygen
How is oxygen removed to form an anaerobic envrionemnt
Uncoupled mitochondria - burns off oxygen
Leghemoglobin in leguemes which binds to oxygen and reduces its concentration
What binds with ammonium to form glutamate?
What are the four most abundant amino acids in the body?
Aspartate, alanine, glutamine, glutamate
What is the process of transferring different amino groups between different molecules?
What does the amino acid react with during transamination?
A keto acid
What keto acid is used to make alanine from glutamate?
Which is converted into alpha ketoglutarate (typically accepts amino groups)
What do all aminotransferases rely on?
Pyridoxal phosphate cofactor
What is the temporary storage of nitrogen?
What is PLP made from?
Where do you find aminotransferases?
In the cell
What is the function of aspartate aminotransferase?
Catalyses the aspartate to oxaloacetate
What is the function of alanine aminotransferase
Catalyses alanine to pyruvate
When do amino acids undergo oxidative catabolism?
Leftover amino acids from normal protein turnover
Excess dietary amino acids
Proteins in the body which are broken down during starvation
Where do you find pepsin?
What breaks down peptides into amino acids in the small intestine?
Peptides -> smaller peptides - Trypsin and chymotrypsin
Smaller peptides -> amino acids - aminopeptidase and carboxypeptidase
What enzymes are membrane bound?