test 4 Flashcards

0
Q

Oswald Avery

A

built off of griffith discovery. concluded protein could not be the transforming factor. said that DNA is the genetic material of a cell.

treated griffiths mixture of heat treated deadly strain and live harmless protein destroying enzymes. the bacterial colonies grown from the mixture were still transformed

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1
Q

Fredrick Griffith

A

“transforming factor” is the genetic material
studied two forms/strains of bacterial species. (pneumonia.) harmless and deadly. deadly became harmless when heated. important discovery came when he mixed heat treated deadly and harmless i to a mouse and the mouse died.

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2
Q

Alfred Hershey and Martha Chase

A

Convinced the science world that DNA is the transforming factor. concluded that phages DNA entered the bacterial cell during infections. DNA must carry the genetic informstion.

did more experiments to prove DNA was the hereditary material. used a batch of infecting phages and mixed it with radioactive isotopes of sulfur to label only the phages protein coats. in another batch they used radioactive isotopes of phosphorus to label DNA. USED A BLENDER.

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3
Q

virus

A

a oackage of nucleic acid wrapped in a protein coat. not made of cells. can only reproduce by infecting another living cell.

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4
Q

bacteripphage

A

a virus that infects bacteria

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5
Q

transforming factor

A

DNA

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6
Q

radioactive isotopes

A

an atom with an unstable nucleus

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7
Q

deoxyribose

A

ring shaped sugar found in nucleotides

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8
Q

nucleotide

A

building blocks (the monomers) of nucleic acid polymers

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9
Q

nitrogenous base

A

a single or double ring of carbon and nitrogen atoms with functional groups

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10
Q

deoxyribonucleic acid (DNA)

A

where heritable genetic information of an organism is stored. a polymer built of monomers called nucleotides.

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11
Q

what is the structure of a single nucleotide?

A

ring shaped sugar called deoxyribose
a phosphate group
a nitrogenous base (single or double ring of carbon)

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12
Q

four nitrogenous bases found in DNA

A

Adenine (A)
Thymine (T)
Cytosine (C)
Guanine (G)

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13
Q

Adenine

A

bonds with Thymine in DNA
bonds with Uricel in RNA
double ring structure (purine)

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14
Q

what are the purines?

A

Adenine and Guanine

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15
Q

what is a purine?

A

larger, double ring structures

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16
Q

what is a Pyrimidine?

A

single ring structures

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17
Q

what are the pyrimidines?

A

cytosine and thymine

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18
Q

Thymine

A

single ringed (pyrimidine)
bonds with Adenine in DNA
not found in RNA
replaced with Uracil in RNA

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19
Q

Cytosine

A
single ringed (pyrimidine)
bonds with guanine in DNA
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20
Q

Guanine

A
double ringed (purine)
bonds with cytosine
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21
Q

Maurice Wilkins and Rosalind Franklin

A

showed that the basic structure of DNA was a helix. photographed DNA using crystallography

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22
Q

Xray Crystallography

A

scattered xrays through the DNA crystal to form an image

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23
Q

James Watson and Francis Crick

A

modeled DNA structure. Used franklins work to create a new model with two strands of nucleotides DOUBLE HELIX. hypothesized nitrogous bases aligned

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24
Double Helix
a twisting shape
25
DNA replication
process of copying the DNA molecule
26
antiparallel
DNA runs in opposite directions | Watson and crick ideas
27
template
a place where it starts
28
DNA polymerases
enzymes that make covalent bonds between the nucleotides of the new DNA strand
29
genotype
an organisms genotype is its genetic make up. a sequence of nucleotide bases in its DNA
30
phenotype
the organism's specific traits which lies in proteins and their wide variety of functions
31
Chargaff
found the base pair rule
32
base pair rule
a goes with t | c goes with g
33
Ribonucleic Acid(RNA)
any nucleic acid whose sugar is ribose rather than the deoxyribose of DNA
34
Differences between DNA and RNA
DNA is a double helix, RNA is a single strand DNA uses deoxyribose, RNA uses ribose DNA doesn't use Uracil, RNA does
35
Uracil
very similar to thymine pyrimidine pairs with adenine in RNA
36
gene
unit of inherited information in DNA
37
transcription
DNA's nucleotide sequence is converted to the single stranded RNA molecule
38
translation
converts nucleic acid language to amino acid language
39
codon
codes for one amino acid
40
how many types of ribonucleic acid (RNA)
3
41
what are the three kinds of RNA
``` Messenger RNA (mRNA) transfer RNA (tRNA) ribosomal RNA (rRNA) ```
42
Messenger RNA (mRNA)
transcribed from a DNA template.
43
RNA polymerase
links the RNA nucleotides together
44
introns
internal noncoding regions
45
peptide bond
bond between amino acids
46
polypeptide
many bonds which makes a protein
47
telomeres
end of a chromosome that has dna that never gets turned into a protein. protects dna as replicated
48
telomerase
enzyme that protects telomeres
49
Linus Pauling
Cal tech professor that was close to figuring out the structure of DNA
50
polymerase
enzyme that makes DNA or RNA
51
Why does DNA need to be converted to RNA?
DNA cant leave the nucleolus.
52
Why do genes become proteins?
genes are units of instructions in DNA taht explain how the protein should be made
53
what is the difference between a gene and DNA
a gene is within the all together dna
54
exon
what from dna in the nucleus is used in the copy
55
intron
unneeded parts that stay in the nucleus
56
helicase
enzyme that separates dna so it can be copied
57
Ribosomal RNA (rRNA)
RNA that goes into the ribosome
58
transfer RNA (tRNA)
rna that folds that travels to the ribosome
59
start codon
AUG. tells where to start
60
stop codon
UC tells to stop
61
mutation
any change in the nucleotide sequence of DNA
62
base substitution
the replacement of one base or nucleotide with another
63
why are base substitutions sometimes harmless?
because in genetic code, several amino acids have more than one codon that code for the same amino acid
64
silent mutation
a change where no change in the protein product would result
65
base insertion
addition of nucleotides
66
base deletion
subtracting a nucleotide
67
why is insertion or deletion of one or more nucleotides in a gene harmful?
because mRNA is read as a series of triplets, adding or subtracting nucleotides may alter the triplet groupings of the genetic message. this will cause them to be regrouped into different codons and will result in a different or non working protein
68
what causes mutations?
when errors occur in DNA replication
69
mutagens
physical or chemical agents that cause mutations
70
trisomy
3 chromosomes instead of 2
71
nondisjunction
event during meiosis in which homologous chromosomes fail to seperate
72
duplication
when part of a chromosome is repeated
73
deletion
occurs when a fragment of a chromosome is lost
74
inversion
reversing a fragment of the original chromosome
75
translocation
when a fragment of a chromosome attaches to a non-homologous chromosome
76
who was down syndrome named after
john langdon down
77
cri du chat
babies cry like a cat
78
ultrasound
sound waves to make an image
79
klienfelters
is the set of symptoms resulting from additional X genetic material in males
80
turners
missing an x chromosome in females
81
amniocentesis
babies fluid is tested for chromosomal disorders
82
karyotypes
the number and visual appearance of the chromosomes in the cell nuclei of an organism or species.
83
tools to diagnose
karyotype, amniocentesis, ultrasound