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Workshop 4 Flashcards

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1
Q

What are the benefits for excellence in research?

A

1- international ranking of the university
2- international & local recognition of the researcher

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2
Q

What’s the ranking of Oxford ,UK

A

1/1906

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3
Q

What’s the ranking of Stanford ,USA

A

2/1906

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4
Q

What’s the ranking of Massachusetts, USA

A

3/1906

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5
Q

What’s the ranking of Harvard,USA

A

4/1906

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6
Q

What’s the ranking of Cambridge, UK

A

5/1906

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7
Q

What’s the ranking of Kuwait university

A

1001-1200/1906

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8
Q

What are international recognition benefits?

A

1- awards given by international organisations
2- invited speaker and international conferences
3- chairman of scientific sessions
4- chief editor , editor , editorial board member of international journals

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9
Q

What are local recognition benefits?

A

1- best researcher awards given by Kuwait university and Kuwait foundation for advancement of sciences
2- Monetary incentives & recognition by research sector , Kuwait university for papers published in Q1 top 25% journals = 1000 KD

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10
Q

What are local recognition for undergraduate students benefits?

A

Dr.Nael Al-Naqib award for best poster in the HSC annual poster conference held every year

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11
Q

What is the mission of RCF/OMICSRU ?

A

1- advance research culture
2- facilitate excellence in research
3- providing state of Art instruments and technical support in?
genomics , proteomics , cellomics

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12
Q

Equipment in genomics lab are ( 16 )🫣

A

1- biosafety cabinets
2- Biorobot M48
3- QIA cube
4- Thermal cylinders
5- Agarose gel electrophoresis
6- Polyacrylamide gel electrophoresis
7- Molecular imaging system
8- QIAxcel advanced system
9- electrophoresis on chip
10- Real time PCR systems
11- Genetic analyzers
12- Microarray systems
13- Primer synthesizer
14- Speed vac
15- Bioinformatics workstation
16- Milli-Q system

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13
Q

What are the equipment in proteomics labs ? ( 14 ) 🫡

A

1-Biorad GS-800 densitometer
2- Agilent 3100 offgel fractionator
3- Isoelectric focusing of proteins / peptides
4- high performance liquid chromatography system
5- Beckman coulter proteomelab PF 2D protein fractionation system
6- LCMS liquid chromatography
7- Mass spectrometry
8- peptide synthesiser symphony X
9- centrifuges (ultra and high speed)
10- Lyophilizer ( ilshin biobase freeze dryer )
11- cell and tissue homogenisers
12- Epoch micro volume spectrophotometer system
13- Immunoassy system (Luminex 200)
14- micro plate reader (UV / VIS spectrometer)

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14
Q

What are the equipment in cellomics labs ? ( 10 ) 😮‍💨

A

1- light microscopes
2- laminar flow hoods
3- CO2 incubators
4- fridges
5- cell viability analyser
6- phase contrast microscopes
7- live cell analysis system
8- automated typing meta system
9- confocal microscopes
10- flow cytometers

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15
Q

Why are biosafety cabinets used?

A

For safe work environment

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16
Q

What Provides personnel & product protection for work through filtered air

A

biosafety cabinets

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17
Q

What is QIAcube used ?

A

Purification of nucleic acids & proteins

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18
Q

A robotic workstation for automated purification of DNA , RNA , proteins,& 1-12 samples can be processed per run

A

QIAcube

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19
Q

Why is biorobot M48 used ?

A

Purification of nucleic acids

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20
Q

Used for purification of DNA & RNA from cells
The instrument has capacity to purify DNA & RNA from 6-48 samples per run

A

biorobot M48

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21
Q

What is used for quantification of DNA , RNA , proteins?

A

1- Implen nanophotometer N60
2- qubit 2.0 fluorometer

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22
Q

Instrument that measures amount of light absorbed after it passes through a solution

A

spectrophotometer

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23
Q

Why are thermal cyclers used ?

A

To synthesise DNA by polymerase chain reaction PCR

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24
Q

In which device is Every cycle is going to double DNA , so it increases the amount of DNA

A

Thermal cyclers

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25
The separation of nucleic acids & proteins
Electrophoresis
26
What separates DNA & RNA molecules using agarose gel Smaller molecules move faster than larger molecules
Agarose gel electrophoresis
27
What is used for protein separation based on size ( molecular weight ) The separated proteins are visualised by staining ( wesrtern blot )
Polyacrylamide gel electrophoresis
28
An automated system for agarose gel & western blot imaging
Biospectrum UVP imaging system
29
A fully automated & sensitive system for high resolution capillary electrophoresis of up to 96 samples per run for DNA & RNA analysis
QIAxcel
30
A faster and more sensitive alternative to agarose & polyacrylamide gels the 2100 bioanalyser / experion automated electrophoresis systems employee microfluidic technology for protein and nucleic acid electrophoresis
Electrophoresis on a chip
31
Examples of Electrophoresis on a chip
2100 bioanalyzer Experion
32
PCR results are analysed in real time after each cycle of ———
amplification
33
Examples of real time PCR systems
ABI7500 ABI7500HT QuantStudio 7 flex
34
fluorescence based DNA sequence analyses instruments using capillary electrophoresis technology
Genetic analysers
35
Examples of Genetic analysers
3130 XL genetic analyser 16 capillary 3500 XL genetic analyser 24 capillary
36
Used to detect gene expression ( DNA transcription into mRNA ) of thousands of genes at the same time
Microarray systems
37
Examples of microarray systems
Affymetrix Gene chip microarray Agilent DNA microarray
38
Using this instrument 12 primers can be synthesised at the same time
mermade 12
39
a short nucleic acid sequence that provides a starting point of DNA synthesis
Primer
40
An instrument used for rapid and efficient concentration and drying of nucleic acids and synthetic oligos (primers)
Thermo Savant DNA 110 speed vac
41
Used to analyse the results of DNA sequencing and microarray data
Bioinformatics workstation
42
Produces pyrogen free and RNasefree ultrapure water from tap water
Millie Q system
43
Provides the concentration of molecules in the bands/dots of a blot
biorad GS 800 densitometer
44
Examples of biorad GS 800 densitometer
X-ray film of southern blot Dot blots Dye-stained PAGE gel
45
Proteins/peptides are separated according to their charge ( isoelectric points ), and the separated components are recovered in liquid phase
Agilent 3100 offgel fractionator ( IEF ) for proteins / peptides
46
2-dimensional separation of protein proteins based on charge and hydrophobicity
Beckman coulter proteomelab PF2D
47
A process of separating components in a liquid mixture
chromatography
48
A liquid sample is injected into a stream of solvent (mobile phase) flowing through a column packed with a separation medium (stationary phase) sample components separate from one another by a process of differential migration as they flow through the column it can detect the presence of Trace compounds or impurities
HPLC system
49
molecules are separated based on differential equilibrium between a mobile phase and the stationary phase
liquid chromatography
50
analyses involves separation of gas phase charged molecules based on their mass to charge rati m / Z.
mass spectrometry
51
Example of peptide synthesiser
Symphony X
52
An instrument that preserves biological materials by removing the water from the sample
Lyophilizer / Ilshin biobase freeze dryer
53
Beckman coulter optima L-100 XP is an ?
Ultra centrifuge
54
thermoscientific sorval WX+ is a ?
high speed centrifuge
55
Omni THQ is a ?
digital tissue homogeniser
56
Sonicator-soniprep 150 plus is a?
cell and tissue homogeniser
57
An instrument that has high throughput up to 16 samples, low volume 2 micro litter, DNA RNA and protein quantification
epoch micro volume spectrophotometer system
58
Example of a microplate reader
UV/Vis spectrometer ( clariostar + )
59
Has Multiplex detection of up to 100 analytes simultaneously applications : quantification of multiple cytokines and other biomarkers
immunoassay system ( Luminex 200 )
60
What instrument produces high contrast images of transparent specimens such as living cells
Carl Zeiss axio imager
61
An instrument for observation and quantification of cell behaviour via real time quantitative live cell analysis
INCUCYTE S3
62
Mention confocal laser scanning microscopes
LSM 700 LSM 800 LSM 980
63
instrument used for high resolution and 3-D viewing
confocal microscopes
64
An instrument with 8 parameters ( 6 fluorescent colours 2 light scatter )
guava easycyte 8HT flow cytometer
65
An instrument with 16 parameters ( 14 fluorescent colour and 2 light scatter )
MACSQuant analyser 16 flow cytometer
66
What are flow cytometers ?
cell cycle apoptosis surface markers biomarkers cytokines

Biology (20 decks)

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