Techniques in Behavioural Neuroscience Flashcards
Ablation and replacement is useful to see whether a gland suspected to be the _____ of a hormone diminishes a behaviour related to it, and whether it is recovered by a replacement of the hormone. However it is ___, and ___, as some glands produce more than a ___ hormone, making findings difficult to interpret
source, invasive, nonspecific, single
using a pharmocological agonist or antagonist is useful for looking at what happens if you ___ or ___ a hormone action. It can also tell you ___ in the body the hormone acts to affect behaviour, but only for ___ and not ____ injections
enhance, inhibit, where, local, systemic
genetic manipulations can tell you the effects of a ___, ___ or ___ of a hormone, and it can also be targeted to specific brain ___ or ____. However, the ___ specificity is bad and __ ___ may occur that are normally unseen. Sometimes gene alteration can be ____
loss, gain, alteration, region, cell, temporal, biological compensation , fatal
Immunoassays can detect and ___ the presence of specific molecules in a sample, and are highly ____, however it is ____.
quantify, sensitive, indirect
Radioimmunoassays can be used to determine the ____ of hormone in a sample, however you need to first obtain a ___ ___ of an antigen, so you need a sample of the hormone you’re measuring first.
concentration, known quantity
ELISAs can also tell you the ___ of hormone in a sample but uses a ______ instead. This also needs a known ____ that will bind the hormone of interest
concentration, spectrometer, antigen
Immunohistochemistry can tell you the ___ of a hormone or receptor, but is worse at detecting the amount than ___ or ____. It also requires ____ the animal or extracting tissue
location, RIA, ELISA, sacrificing
autoradiography measures the ___ and ___ or receptors in tissue
density, location
in situ hybridization identifies the cells or tissues producing the ___ that encodes a specific protein. It tells you whether that substance is produced in that specific ____. It also requires _____ of the animal
mRNA, tissue, sacrificing
blot tests show you if a particular ____ or ___ __ is in a given tissue. ____ blots detect DNA, southern blots detect _____ and ____ blots detect ____. This technique is not ideal for precise ____ of the protein because the tissue needs to be ____
protein, nucleic acid, northern, RNA, western, proteins, localization, homogenized
fiber photometry can be used to measure the amount of hormone or ____ signalling in ___ ___, behaving animals. It has better ___ resolution that microdialysis, but is not as precise of a measure of molecule ___. It only shows ___ changes in the amount of a molecule over time
neurotransmitter, real time, temporal, concentration, relative
microdialysis allows you to determine the ____ of hormone in real time behaving animals, which is not bad for measuring _____ ____ hormones. However, you need a _____ technique with it to determine the amount in a sample, and the ___ ___ is bad, making measuring _____ concentrations difficult
concentration, slow acting, secondary, temporal resolution, neurotransmitter
