Techniques in Behavioural Neuroscience Flashcards

1
Q

Ablation and replacement is useful to see whether a gland suspected to be the _____ of a hormone diminishes a behaviour related to it, and whether it is recovered by a replacement of the hormone. However it is ___, and ___, as some glands produce more than a ___ hormone, making findings difficult to interpret

A

source, invasive, nonspecific, single

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2
Q

using a pharmocological agonist or antagonist is useful for looking at what happens if you ___ or ___ a hormone action. It can also tell you ___ in the body the hormone acts to affect behaviour, but only for ___ and not ____ injections

A

enhance, inhibit, where, local, systemic

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3
Q

genetic manipulations can tell you the effects of a ___, ___ or ___ of a hormone, and it can also be targeted to specific brain ___ or ____. However, the ___ specificity is bad and __ ___ may occur that are normally unseen. Sometimes gene alteration can be ____

A

loss, gain, alteration, region, cell, temporal, biological compensation , fatal

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4
Q

Immunoassays can detect and ___ the presence of specific molecules in a sample, and are highly ____, however it is ____.

A

quantify, sensitive, indirect

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5
Q

Radioimmunoassays can be used to determine the ____ of hormone in a sample, however you need to first obtain a ___ ___ of an antigen, so you need a sample of the hormone you’re measuring first.

A

concentration, known quantity

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6
Q

ELISAs can also tell you the ___ of hormone in a sample but uses a ______ instead. This also needs a known ____ that will bind the hormone of interest

A

concentration, spectrometer, antigen

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7
Q

Immunohistochemistry can tell you the ___ of a hormone or receptor, but is worse at detecting the amount than ___ or ____. It also requires ____ the animal or extracting tissue

A

location, RIA, ELISA, sacrificing

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8
Q

autoradiography measures the ___ and ___ or receptors in tissue

A

density, location

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9
Q

in situ hybridization identifies the cells or tissues producing the ___ that encodes a specific protein. It tells you whether that substance is produced in that specific ____. It also requires _____ of the animal

A

mRNA, tissue, sacrificing

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10
Q

blot tests show you if a particular ____ or ___ __ is in a given tissue. ____ blots detect DNA, southern blots detect _____ and ____ blots detect ____. This technique is not ideal for precise ____ of the protein because the tissue needs to be ____

A

protein, nucleic acid, northern, RNA, western, proteins, localization, homogenized

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11
Q

fiber photometry can be used to measure the amount of hormone or ____ signalling in ___ ___, behaving animals. It has better ___ resolution that microdialysis, but is not as precise of a measure of molecule ___. It only shows ___ changes in the amount of a molecule over time

A

neurotransmitter, real time, temporal, concentration, relative

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12
Q

microdialysis allows you to determine the ____ of hormone in real time behaving animals, which is not bad for measuring _____ ____ hormones. However, you need a _____ technique with it to determine the amount in a sample, and the ___ ___ is bad, making measuring _____ concentrations difficult

A

concentration, slow acting, secondary, temporal resolution, neurotransmitter

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