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MTAP 2 Serology > 1.1 Introduction to Serology > Flashcards

1.1 Introduction to Serology Flashcards

1
Q

Serology is the study of what component of the blood?

A

Serum

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2
Q

Preservation of Serum:

Physical: Refrigerate for ___hrs at ____ C

A

72

4-6

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3
Q

Preservation of Serum:

Chemical: add 0.001g _________ per ml serum or 5% _______ or _______ at 0.1ml/ml of serum

A

Merthiolate powder

Phenol
Tricresol

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4
Q

Inactivation of Serum:

Complements are naturally seen in the serum

T or F

A

T

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5
Q

Inactivation of Serum:

What is the purpose of inactivating the serum?

A

Inactivates complement

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6
Q

Inactivation of Serum:

Complements are part of innate immunity
T or F

A

T

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7
Q

Inactivation of Serum:

Physical: Heat serum at 56 C for __ mins or heat at ______ for 3-5 mins only

A

30

60-62 C

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8
Q

Inactivation of Serum:

Chemical: Add ______ chloride

A

Choline

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9
Q

Example of Application for inactivation of Serum?

A

Test for Syphilis such as:
- VDRL
- RPR

Complement Fixation test

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10
Q

For RPR example for inactivation of serum, how do you inactivate it?

A

Add Choline chloride

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11
Q

Inactivating complement in VDRL is through?

A

Heating

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12
Q

Inactivating serum for complement fixation is done through?

A

Heating (Physical)

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13
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Binding of Ag-Ab; non-visible reaction; very sensitive and specific

A

Primary

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14
Q

Immunologic / Serologic reactions:

demonstrable Ab-Ag reaction Visible

A

Secondary

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15
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Primary can be detected through label

T or F

A

T

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16
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Labeled immunoassays can be seen in secondary test

T or F

A

F

Primary

17
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

ELISA, RIA, Immunohistochemistry

A

Primary

18
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Agglutination, Hemolysis, Neutralization

A

Secondary

19
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Best to detect analytes in the serum

A

PRimary

20
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Immunologically in vivo; biologic reaction is detectable

A

Tertiary

21
Q

Immunologic / Serologic reactions: (Primary, Secondary, Tertiary)

Phagocytosis, opsonization, and chmotaxis

A

Tertiary

MTAP 2 Serology (29 decks)

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