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MTAP 2 Serology > 11.2 Monospot Test, Paul Bunnell, Davidsohn Differential Test > Flashcards

11.2 Monospot Test, Paul Bunnell, Davidsohn Differential Test Flashcards

1
Q

Principle: Hemagglutination

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

a

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2
Q

Screening test for detection for heterophile antibody

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

b

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3
Q

confirm the identity of the
heterophile antibodies.

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

c

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4
Q

Not for identification of specific heterophile antibody

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

b

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5
Q

Reagent: 2% Sheep RBC

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

b

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6
Q

Reagent:

→ Horse RBC
→ Guinea Pig Kidney
→ Ox/Beef RBC

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

a

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7
Q

Reagent:

→ Guinea Pig Kidney
→ Beef RBC
→ Horse RBC
→ Sheep RBC

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

c

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8
Q

In Monospot test

Receptor for Forssman, IM

a. Horse RBC
b. Guinea Pig Kidney
c. Ox/Beef RBC

A

a

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9
Q

In Monospot test

Receptor for Forssman, IM, SS

a. Horse RBC
b. Guinea Pig Kidney
c. Ox/Beef RBC

A

c

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10
Q

In Monospot test

Receptor for Forssman, SS

a. Horse RBC
b. Guinea Pig Kidney
c. Ox/Beef RBC

A

b

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11
Q

In Davidsohn Differential Test:

Receptor for (Absorb) Forssman, SS

a. Guinea Pig Kidney
b. Beef RBC
c. Horse RBC
d. Sheep RBC

A

a

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12
Q

In Davidsohn Differential Test:

Receptor for (Absorb) Forssman, IM

a. Guinea Pig Kidney
b. Beef RBC
c. Horse RBC
d. Sheep RBC

A

c

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13
Q

In Davidsohn Differential Test:

Receptor for (Absorb) IM, SS

a. Guinea Pig Kidney
b. Beef RBC
c. Horse RBC
d. Sheep RBC

A

b

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14
Q

In Davidsohn Differential Test:

Receptor for (Absorb) Forssman, IM, SS

a. Guinea Pig Kidney
b. Beef RBC
c. Horse RBC
d. Sheep RBC

A

d

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15
Q

Testing Procedure
* Reagent 1 (R1): Guinea Pig Kidney Antigen (GPK antigen)
* Reagent 2 (R2): Beef RBC
* Sample: Serum
1. Label one side of slide as 1 then label other side as 2
2. Add one drop of serum on left side (1) and right side (2) of slide
3. Add one drop of horse RBC on each side
4. Add one drop of GPK on left side (1)
5. Add one drop of Beef RBC on right side (2)
6. Mix then check for stronger reaction and interpret

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

a

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16
Q

In Davidsohn Differential Test:

Absorbed by GPK: (+)

Absorbed by Beef RBC: (+)

a. Forssman Ab
b. Serum Sickness Ab
c. IM Ab

A

b

17
Q

In Davidsohn Differential Test:

Absorbed by GPK: (-)

Absorbed by Beef RBC: (+)

a. Forssman Ab
b. Serum Sickness Ab
c. IM Ab

A

c

18
Q

In Davidsohn Differential Test:

Absorbed by GPK: (+)

Absorbed by Beef RBC: (-)

a. Forssman Ab
b. Serum Sickness Ab
c. IM Ab

A

a

19
Q

In Davidsohn Differential Test:

Agglutination pattern After absorption with GPK (+)

Agglutination pattern after absorption with Beef RBC: (-)

a. Forssman Ab
b. Serum Sickness Ab
c. IM Ab

A

c

20
Q

In Davidsohn Differential Test:

Agglutination pattern After absorption with GPK (-)

Agglutination pattern after absorption with Beef RBC: (-)

a. Forssman Ab
b. Serum Sickness Ab
c. IM Ab

A

b

21
Q

In Davidsohn Differential Test:

Agglutination pattern After absorption with GPK (-)

Agglutination pattern after absorption with Beef RBC: (+)

a. Forssman Ab
b. Serum Sickness Ab
c. IM Ab

A

a

22
Q

Sample: inactivated Serum
a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

b

23
Q

Determined through serial dilution

a. Monospot Test (Citrated Horse Cells Test)
b. Paul Bunnell Test
c. Davidsohn Differential Test

A

b

24
Q

In Paul Bunnell test:

Significant titer: 1:56

Presumptive titer: 1:224

T or F

A

T

25
Q

In Paul Bunnell test:

Significant titer: Indicates presence of heterophile antibodies

Presumptive titer: Presumptive for IM

T or F

A

T

26
Q

In Monospot test:

Presence of Heterophile antibodies: _____ (What is the positive indicator?

A

Stronger agglutination on Square 1 or side

MTAP 2 Serology (29 decks)

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