4.1 Complement Fixation Flashcards
Specimen prep for complement fixation includes _______ of serum to inactivate complement
Heating
In complement fixation: ___ combines with an Ab in the presence of the complement, and the complement is “FIXED” by the immune complex
Ag
Complement fixation is the inability of the complement to react with cells sensitized with other immune complexes
T or F
T
What is the analyte of interest for complement fixation?
Complement fixing Ab
What type of antibody is excellent in fixing the complement?
IgM
(Complement fixing Ab)
2 systems involved in complement fixation:
Test system/ __________ System
Indicator system / _____________ system
Bacteriolytic
Hemolytic
Components of complement Fixation:
Beef Heart Extract, Bacterial Ag
a. Known target Ag Reagent
b. Complement
c. Hemolysin Amboreceptor
d. Indicator Cells
a
Components of complement Fixation:
Sensitized sRBC
a. Known target Ag Reagent
b. Complement
c. Hemolysin Amboreceptor
d. Indicator Cells
d
Components of complement Fixation:
Guinea Pig (Best Source)
a. Known target Ag Reagent
b. Complement
c. Hemolysin Amboreceptor
d. Indicator Cells
b
Components of complement Fixation:
The Ab used to sensitized indicator cell
a. Known target Ag Reagent
b. Complement
c. Hemolysin Amboreceptor
d. Indicator Cells
c
Components of complement Fixation:
Rabbit Antisera
a. Known target Ag Reagent
b. Complement
c. Hemolysin Amboreceptor
d. Indicator Cells
c
What is the positive result for Complement fixation?
No hemolysis
In the indicator cells, the sensitized RBC is sheep RBC that is coated with IgG
T or F
T
What part of the antibody do complement bind?
Fc
