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MTAP 2 Serology > 5.2 Indicator Labels for Immunoassays > Flashcards

5.2 Indicator Labels for Immunoassays Flashcards

1
Q

Most sensitive, with unstable nuclei and emit radiation spontaneously

A

Radioactive Isotopes

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2
Q

Radioactive isotopes:

These are commonly used isotopes in the clinical laboratory

a. H3
b. H4
c. I 125
d. I 124

A

a and c

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3
Q

Application of Radioactive isotopes?

A

Radioimmunoassays

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4
Q

Advantage of Radioactive isotopes?

A

Most sensitive

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5
Q

Disadvantage of Radioactive isotopes?

A

Hazardous

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6
Q

What is being measured in Radioactive isotopes? What apparatus is used for measuring this?

A

Radioactivity

Scintillation Counter

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7
Q

Scintillation counter is usd to measure beta rays

T or F

A

F

Gamma

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8
Q

Types of immunoassay formats:

Molecules that absorb light and becomes transiently excited.

A

Fluorochromes

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9
Q

In fluorochromes, the excited molecule becomes stable and is emitted as Ultraviolet light

T or F

A

F

Visible light

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10
Q

Example of Fluorochromes:

FITC
Acridine orange

T or F

A

T

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11
Q

What is the apparatus for Fluorochrome?

A

FLuorometer

Fluorescence microscope

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12
Q

Application for Fluorochrome?

A

Immunofluorescence

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13
Q

Most common label that acts upon the substrate added in te reaction leading to a change that can be measured

A

Enzymes

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14
Q

Enzymatic activity can be a colored product

T or F

A

T

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15
Q

Application for enzymes?

A

ELISA

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16
Q

Most commonly use enzymes?

A

Horseradish peroxidase

17
Q

Color developer for Enzymes

A

Chromogen

18
Q

Example of Chromogen used in Enzymes?

A

DAB (Diaminobenzidine)

TMB

AEC

19
Q

Most commonly used Chromogen?

A

DAB

20
Q

What is the apparatus used for ELISA to Quantitate?

A

Spectrophotometer

21
Q

This labels employed in automated instruments; stable and nontoxic reagents

A

Chemiluminescent labels

22
Q

What type of label is this?

Acridinium Esters, Luminol, PEroxyoxalates, Dioxetanes

A

Chemiluminescent Labels

MTAP 2 Serology (29 decks)

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