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MTAP 2 Serology > 2.3 Immunoelectrophoresis > Flashcards

2.3 Immunoelectrophoresis Flashcards

1
Q

In immunoelectrophoresis:

is a single-diffusion techniques that incorporates electricity to enhance result

T or F

A

F

Double diffusion

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2
Q

In immunoelectrophoresis:

Serum: Source of?

A

Ag

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3
Q

In immunoelectrophoresis:

Trough: place the?

A

Ab (Antisera)

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4
Q

In immunoelectrophoresis:

Double diffusion occurs at ______ angles

A

right

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5
Q

In immunoelectrophoresis:

Expected result?

A

Precipitin line / arc

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6
Q

In immunoelectrophoresis:

Qualitative or Quantitative?

A

Quali

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7
Q

In immunoelectrophoresis:

Procedures:

Prepare agarose gel

Antigens migrate under an electric current

Reagent Ab is added in the _______:Diffusion through gel

Formation of Precipitin Arc

A

Ab

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8
Q

Counter immunoelectrophoresis:

is a double immunodiffusion with electricity and a pH of _____ of the medium

A

8.6

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9
Q

Counter immunoelectrophoresis:

Antigen and antibody placed?

A

Parallel to each other (On one side and the other side)

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10
Q

Counter immunoelectrophoresis:

Is a 2 dimesion

T or F

A

F

1 dimension

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11
Q

Counter immunoelectrophoresis:

End result?

A

Precipitin line

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12
Q

Counter immunoelectrophoresis:

Rapid and sensitive

T or F

A

F

Not sensitive

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13
Q

Counter immunoelectrophoresis:

Quali or Quanti?

A

Quali

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14
Q

Rocket Electrophoresis:

is also known as?

A

Laurell electrophoresis

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15
Q

Rocket immunoelectrophoresis:

__________ is incorporated in the Agarose and the unknown ________ is placed on the well and electrophoresed

A

Antiserum

Antigen

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16
Q

Rocket immunoelectrophoresis:

The height of the rocket measured from the well to the apex and is indirectly proportional to the amount of Ag

T or F

A

F

Directly

17
Q

Rocket immunoelectrophoresis:

Quali or Quanti?

A

Quanti

18
Q

Rocket immunoelectrophoresis:

Is a Single immunodiffusion

T or F

A

T

19
Q

Rocket immunoelectrophoresis:

Is 1 dimension

T or F

A

T

20
Q

Immunofixation electrophoresis :

First described by?

A

Alper and Johnson

21
Q

Immunofixation electrophoresis :

2 Stage Technique:

Use to separate the proteins (Ag)

A

Electrophoresis

22
Q

Immunofixation electrophoresis :

2 Stage Technique:

Similar o immunoelectrophoresis except that antiserum is applied Directly rather than placed in the trough

A

Immunoprecipitation

23
Q

Immunofixation electrophoresis :

Agarose and cellulose acetate can be used

T or F

A

T

24
Q

Immunofixation electrophoresis :

Example?

A

Western, Southern, and Northern Blotting

25
Q

Immunofixation electrophoresis :
Procedures:

Aliquots of the patient’s serum placed in each well

Perform electrophoresis: Separation of ____

A

Ag

26
Q

Immunofixation electrophoresis :

In western blotting: What is being detected?

A

Antibody

MTAP 2 Serology (29 decks)

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