2.2 Types of Precipitation reactions

Question 1

Precipitation in liquid:

Test tube method: Tubidimetry
Slide method: Use microscope

T or F

Answer 1

T

Question 2

Precipitation in liquid:

Capillary method: Dense granulation in the metaphase

T or F

Answer 2

F

Interphase

Question 3

Precipitation in liquid:

Ring test: Precipitin ____ formation

Answer 3

Ring

Question 4

Precipitation in liquid:

Example of Ring test?

Answer 4

Ascoli Test

Question 5

Precipitation in liquid:

Where do Ascoli test is used?

Answer 5

Anthrax (B. anthrasis)

Question 6

Precipitation in Semi solid Medium contains?

Answer 6

1% Agarose

Question 7

Single immunodiffusion: 1 object Movement
Single Dimension: 1 directions

T or F

Answer 7

T

Question 8

Single immunodiffusion, Single dimension is also known as?

Answer 8

Oudin test

Question 9

Single immunodiffusion, Single dimension:

PAtient sample with ________ and the agar is impregnated with _________

Answer 9

Ag

Antisera (Ab)

Question 10

Single immunodiffusion, Single dimension:

(+) result?

Answer 10

Precipitin bond/ring

Question 11

Single immunodiffusion, Single dimension:

Only antigen moves

T or F

Answer 11

T

Question 12

Double immunodiffusion: 2 objects move

T or F

Answer 12

T

Question 13

Double diffusion in One Dimension is also known as?

Answer 13

Oakley Fulthorpe method

Question 14

Double diffusion in One Dimension:

_____ is incorporated in agar, poured into a tube and allowed to harden

Answer 14

Ab

Question 15

Double diffusion in One Dimension:

A second layer of agar without ________ is placed above and allowed to solidify

Answer 15

antibody

Question 16

Double diffusion in One Dimension:

___________ is placed above the agar (The very top)

Answer 16

Sx-Ag

Question 17

Double diffusion in One Dimension:

(+) Result: The precipitin band appears in the?

Answer 17

Agar without the antibody

Question 18

Immunodiffusion in 2 dimension has 2 or more movement

T or F

Answer 18

T

Question 19

Immunodiffusion in two dimensions is usually plated

T or F

Answer 19

T

Question 20

Single diffusion, Double Dimension is also known as

Answer 20

Radial immunodiffusion

Question 21

Single diffusion, Double Dimension: Uses a plate containing agar with?

Answer 21

Ab

Question 22

Single diffusion, Double Dimension:

___________ is placed on the wells

Answer 22

Ag

Question 23

Single diffusion, Double Dimension:

The diameter of the precipitin is ___________ proportional to the concentration of the target antigen

Answer 23

Directly

Question 24

Single diffusion, Double Dimension:

Antigen moves toward the agar gel

T or F

Answer 24

T

Question 25

Single diffusion, Double Dimension:

Methods:

Also known as the end point method where the square of the diameter is directly proportional to the conc. of Ag

Answer 25

Mancini

Question 26

Mancini usually takes?

Answer 26

24-72 hrs

Question 27

Single diffusion, Double Dimension:

Method:

A kinetic method where the diameter is proportional to the log of the concentration of Ag

Answer 27

Fahey Mckelvey

Question 28

Fahey Mckelvey usually takes?

Answer 28

18 hrs

Question 29

Double diffusion, double dimension is also known as?

Answer 29

Ouchterlony or Double Angular immunodiffusion

Question 30

Double diffusion, double dimension:

is also in the plated media

T or F

Answer 30

T

Question 31

Double diffusion, double dimension:

Diffusion of both?

Answer 31

Ag / Ab

Question 32

Double diffusion, double dimension:

Diffusion of 3 reagents placed in wells (___ Ag and ___ Ab)

Answer 32

2 and 1

Question 33

Double diffusion, double dimension:

Diffusion in all directions

T or F

Answer 33

T

Question 34

Double diffusion, double dimension:

This test can be used to find our the relationship between antibody

T or F

Answer 34

F

Antigen

Question 35

Double diffusion, double dimension:

Has a complicated diffusion patterns

T or F

Answer 35

T

Question 36

Double diffusion, double dimension:

This result forms a perfect precipitin arc

Answer 36

Identity

Antigens is very identical

Question 37

Double diffusion, double dimension:

This result forms a cross instead of Arc

Answer 37

Non identity

Question 38

Double diffusion, double dimension:

This result forms a spur

Answer 38

PArtial identity

Antigens are identical but not the same