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Flashcards in 2012 biochem midterm Deck (37)
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1

Which Histone is NOT present in the nucleosome core?
a. H1
b. H2
c. H3
d. H4
e. H5

a. H1

2

We have 1 primer amplified within 20 cycles of PCR techniques. How many folds do we have?
a. 20 folds
b. 200 folds
c. 2,000 folds
d. 20,000 folds
e. 200,000 folds

a. 20 folds

3

What process will be affected if a mutation occurs in the census sequence AAUAAA?
a. 5’ Capping
b. Addition of 3’ Poly-A-tail
c. Splicing
d. Removal of Introns
e. RNA Initiation

b. Addition of 3’ Poly-A-tail

4

Taking gene from one person & incorporating it with another person. What is it called?
a. Recombinant DNA
b. Restriction modification
c. DNA mutation
d. DNA signaling

a. Recombinant DNA

5

What’s the property of Collagen?
a. Hypo-methylated
b. Hyper-methylated
C. Contain 2 identical polypeptide chain
D. Heavily glycosylated
E. Stabilized by hydrophobic interactions

D. Heavily glycosylated

6

Why’s the reaction stopped when we used ddNTP?
a. 3’-OH is used
b. 3’-H is used
c. Primer is hydrolyzed
d. The annealing temperature is too high

b. 3’-H is used

7

What disturbs the secondary structure of the protein?
a. Lysine
b. Isolysine
c. Proline
d. Glycine

c. Proline

8

Gum Bleeding is caused by a deficiency of Vitamin C. What biochemical change will occur?
a. A decrease in the hydroxylation of Proline
b. A decrease in the hydroxylation of Lysine
c. A decrease in the hydroxylation of Glycine

a. A decrease in the hydroxylation of Proline

9

Lack of which OH prevent ddNTP?
a. 1’-OH
b. 2’-OH
c. 3’-OH
d. 4’-OH
e. 5’-OH

c. 3’-OH

10

What helps insert a protein with M6P in Golgi?
a. Lysosomes
b. Cytoplasm
c. Membrane
d. ER
e. Receptor

a. Lysosomes

11

Aminoacyl tRNA (aa-tRNA) that carry amino acid to translation are highly reactive. What factor stabilizes them in the cytoplasm?
a. EFG
b. EF-1
c. EF-3
d. Tu
e. Ts

d. Tu

12

How do suicide enzymes affect substrate?
a. Bind to allosteric site
b. Bind to active site
c. Disrupt synthesis
d. Disrupt degradation

b. Bind to active site

13

What stimulates glycogen synthesis in muscles?
a. Ca
b. Epinephrine
c. Norepinephrine
d. Insulin
e. Glucogon

d. Insulin

14

What’s the key feature in prion structures?
a. Change in primary protein
b. Excessive beta sheets
c. Increase in alpha-helix
d. Decrease in proline

b. Excessive beta sheets

15

What’s the property of highly substrate active sites?
a. High Km
b. Vm
c. Sigmoid-shaped

c. Sigmoid-shaped

16

Who has the highest % of having CF?
a. White
b. Hispanic
c. Black
d. Asian

a. White

17

During protein translation, an antibiotic analog aa-tRNA occupies the P-site. What’s inhibited?
a. Elongation
b. Initiation
c. Termination
d. Translocation

b. Initiation

18

A gene coding for lysosomal proteins is missing leader sequence. Where will the protein go?
a. Cytoplasm
b. Secretory
c. Mitochondria
d. Nucleus
e. Lysosome

e. Lysosome

19

What’s the indication of the change of β-Hemoglobin to sickle cell anemia?

Mutation of one AA

20

What kind of mutation does the gene encoding phenylalanine hydroxylase enzyme have?
a. Deletion
b. Splice
c. Nonsense
d. Insertion
e. Missense

e. Missense

21

What’s the characteristic of CREB?

Transcription Factor

22

Which enzyme activity signals Energy cell deficiency?
a. Na/K ATPase
b. hexokinase
c. AMPase
d. Glycogen synthesis
e. Protein Kinase A

a. Na/K ATPase

23

What’s the type of inheritance of CF?
a. X-linked Recessive
b. Autosomal Dominant
c. Autosomal Recessive
d. X-linked Dominant

c. Autosomal Recessive

24

What is the characteristic of metaphasic chromosome?
a. Naked DNA molecule w/o any histones
b. Single Stranded DNA Molecule
c. A DNA molecule that is synthesized at the G1 stage of cell cycle
d. 4 strands of DNA with histones
e. 1 strand of DNA with histones

d. 4 strands of DNA with histones

25

What’s the characteristic of HK (housekeeping gene)?
a. Continuously expressed
b. Regulated
c. Highly methylated Poorly acetylated
d. Highly phosphorylated

a. Continuously expressed

26

In CF, What’s the % of the affected offspring if the 2 parents were carriers?
a. 0
b. 25
c. 50
d. 75
e. 100

b. 25

27

Which DNA sequence is used for DNA fingerprinting?
a. Microsatellite DNA
b. Promoter region
c. Hypervariable minisatellite
d. Telomeric DNA Coding DNA

c. Hypervariable minisatellite

28

Which of the following is a characteristic of actively transcribed gene?
a. Found in euchromatic
b. Insensitive to α-amantin
c. Transcribed DNA Polymerase
d. Poorly acetylated

a. Found in euchromatic

29

What general characteristic is shared by most highly regulated intracellular enzymes?
a. Catalyze fastest step in the pathway
b. Rapidly degraded in the cytosol
c. Can be applied to michaelis-menten equation
d. Unchanged by phosphorylation

b. Rapidly degraded in the cytosol

30

What’s the function of a restriction enzyme?
a. Prevent formation of sugar-phosphate backbone
b. Breakdown DNA & RNA into nucleotides
c. Prevent formation of chemical bonds between pairs of nucleotides
d. Synthesis of DNA during replication
b. Breakdown of DNA molecules between specific nucleotides

b. Breakdown of DNA molecules between specific nucleotides