2.5 Inflammation and adhesion Flashcards Preview

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Flashcards in 2.5 Inflammation and adhesion Deck (87)
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61

rolling-adhesion-extravasation strategies are to

increase the number of receptors and increase affinity of receptors for ligands

62

endothelium molecules

p-selectin,
e-selectin,
ICAM-1,
VCAM1,
CD 34/GlyCam,
CD31

63

P-selectin

sialyl-lewis X and PSGL-1 (rolling: N, M, L)

64

E -selectin

sialyl-lewis X (rolling adhesion: N, M, L)

65

ICAM - 1

CD11/18 (beta2 integrins, aka LFA1 and Mac-1) (adhesion, arrest and transmigration: all leukocytes)

66

Vcam-1

VLA4 (alpha4beta1) (integrins) and LPAM-1(alpha4beta7) (adhesion E,M,L)

67

CD-34/GlyCam

L-selectin (rolling; lymphocyte homing: L)

68

CD31 (PECAM)

CD31 (transmigration:all)

69

Selectins

have an extracellular sugar binding domain similar to lectins. They bind sialyated oligosaccharides

70

knock out L selectin

poorly formed LNs and few t cells

71

knock out either E or P selectin

mind effects bc the other compensates

72

knock out both e and p selectin

severe effects

73

immunoglobulin like molecules

ICAM-1, VCAM-1 (both bind integrins on leukocytes)

74

integrins

transmembrane heterodimer glycoproteins with alpha and beta chains

75

beta 2 integrins

LFA1 and MAC1 (bind ICAM1)

76

beta 1 integrins

VLA4 and LPAM1 (bind to VCAM1)

77

1. histamine, thrombin and PAF induce

the redistribution of P-selectin from intracellular sotres (weible-palade bodies) to the surface (takes minutes)

78

2. Tnf and IL1 induce

the synthesis of E-selectin ICAM1, VCAM1 (takes 1-2 hours)

79

3.luekocyte specific chemokines alter

conformation of LFA-1 and MAC-1 increasing the affinity of binding to ICAM 1.
requires ICAM1 and synthesis of chemokines by othercells (late response)

80

4. early endothelial activation i.e.

rolling (selectins and sialyl-lewis X)

81

5. later synthesis of

endothelial adhesion molecules and increased affinity of LFA1 and MAC1 --firm adhesion

82

7. CD31, ICAM and integrins result in

transmigration

83

genetic deficiency called leukocyte adhesion deficiency type 1 (LAD1)

def in LFA1 and Mac

84

LAD2

def in sialyl-LewisX - big problem with leukocyte accumulation

85

antibidies to adhesion molecules can block inflammation like

Natalizumab

86

natalizumab

humanized monoclonal AB to VLA4 (alpha4beta1interin) to treat MS

87

negative effect of natalizumab

bind up all the vla4? So no adhesion of leukocytes