Human Bio Unit 1 - Chromatography Flashcards
0
Q
How does chromatography work?
A
Add a solvent to to the paper
Add solvent to the paper at the base line
Chemicals are separated by the affinity to the solvent
Can work out the RF value of each chemical to work out what it is
1
Q
Uses of chromatography
A
Identify unknown chemicals
2
Q
RF value
A
Used to identify what the “blobs” are on the chromatography paper
3
Q
How to work out the RF value of a chemical
A
Measure from the base line to the front line (1)
Measure from the base line to the top of the “blob” you want (2)
2/1 = RF value
4
Q
Affinity
A
A strong liking for something
