BACTE LAB FE

Question 1

This is the most commonly used stain for yeasts and molds.

Answer 1

Lactophenol Cotton Blue (LPCB)

Question 2

Chloramphenicol is a broad spectrum antibiotic added to SDA in the culture of fungi in order to:

Answer 2

Inhibit bacterial growth

Question 3

Pigmentation of fungi is due to the presence of what structures?

Answer 3

Spores

Question 4

The initial hypha that develops from a conidium or spore is called:

Answer 4

Germ tube

Question 5

What is the widely used method of enumerating or counting bacteria in meat and milk samples?

Answer 5

Total Plate Count (TPC)

Question 6

This microorganism from undercooked hamburger meat can cause fatal kidney failure or hemolytic uremic syndrome.

Answer 6

E. coli

Question 7

The two principal forms of fungi are the mold and the yeasts. The main element of the vegetative or growing form of the mold is known as what?

Answer 7

Hypha

Question 8

Molds produce intertwined threads that form parts that arise above the surface and are referred to as the ___.

Answer 8

Aerial (hyphae)

Question 9

The following fungi belong to phyla which do NOT produce sexual spores:

Answer 9

Deuteromycota and Chytridiomycota

Question 10

The following are sexual spores produced by fungi EXCEPT:

Answer 10

Chlamydospore

Question 11

What is the standard medium for culturing fungi in a laboratory setting?

Answer 11

Sabouraud Dextrose Agar (SDA)

Question 12

What are the 2 main morphological forms of fungi?

Answer 12

Branching filaments and Unicellular yeasts

Question 13

Among the given, which of the following are NOT asexual spores?

Answer 13

Ascospores and Basidiospores

Question 14

This is added to the medium to inhibit the growth of nonpathogenic species of fungi.

Answer 14

Cyclohexidine

Question 15

This reagent is added to clinical specimens to hydrolyze polysaccharides and leave hyphae intact.

Answer 15

KOH (Potassium Hydroxide)

Question 16

What technique is used for rapid mounting and has a disadvantage of its transparency fading over time?

Answer 16

Adhesive tape method

Question 17

A simple method that permits the growth of fungi gathered from the environment (in situ method.)

Answer 17

Slide culture technique

Question 18

What is the term used to describe the septate hyphae of Zygomycetes members?

Answer 18

Coenocytic

Question 19

A microorganism that is a common contaminant of hamburger meat.

Answer 19

E. coli

Question 20

What medium is utilized for the total coliform test?

Answer 20

Brilliant Green Broth

Question 21

True/False: Gas production with growth in EC broth in 24 ± 2 hours confirms fecal coliform presence.

Answer 21

True

Question 22

The immediate source of most bacteria on carcasses after slaughter is the:

Answer 22

Hide and skins

Question 23

Total plate count can be done in the following products EXCEPT:

Answer 23

Potable water

Question 24

Spore-bearing septate hyphae form a brush and unbranched conidia chains from flask-shaped phialides. Which fungus is this?

Answer 24

Penicillium notatum

Question 25

The following microorganisms are capnophiles EXCEPT:

Answer 25

Aspergillus niger

Question 26

E. coli can adapt in intestinal and extraintestinal habitats because it is aerobic and can be anaerobic in its respiration?

Answer 26

True

Question 27

The technique used in bacteriological analysis of wastewater is the ___ method.

Answer 27

Most Probable Number (MPN) or Multiple Tube Fermentation Technique (MTFT)

Question 28

The usual asexual division in yeasts is by:

Answer 28

Formation of buds

Question 29

Dermatophytes are pathogens causing what type of infection in animals and man?

Answer 29

Ringworm

Question 30

The fungus Cryptococcus neoformans utilizes what substance present in the droppings of birds?

Answer 30

Creatinine