BB Lab (Blood Donor Screening) Flashcards

1
Q

Required by every hospital in the Philippines equipped with Blood Banking services.

A

standard protocol on screening blood donors

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2
Q

The standard protocol on screening blood donors ia based on the provisions provided by the national voluntary blood services program duly instituted by the ___________________________.

A

Department of Health

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3
Q

Why is blood donor screening done?

A

To avoid the risk of blood transmissible diseases by careful examination of the potential donors.

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4
Q

Laboratory screening includes: (9)

A

Weight Determination
Blood Pressure Determination
Pulse Rate Determination
Temperature Determination
Hemoglobin Determination
Hematocrit Determination
Blood Type Determination
Tests for the Blood Transmissible Disease
Bleeding Demonstration

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5
Q

Weight Determination:

  1. These should be removed as it may add to the standard weight requirement for a donor.
  2. Position of the donor on a weighing scale
  3. This should be determined
  4. The weight should be recorded in what units? On what?
A
  1. Shoes, heavy clothing, apparel
  2. Standing straight
  3. Weight
  4. Pounds and kilograms on Blood Donor Interview Data Sheet
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6
Q

1 kg is equivalent to ______ pounds

A

2.2

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7
Q

This tells you the maximum amount of blood that can be drawn from a donor.

A

weight

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8
Q

The maximum allowble blood is suspended in _______________ present in the primary blood bag.

A

63 mL of anticoagulant

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9
Q

If the weight is <110 pounds or <50 kilograms, the blood bank may …

A

collect lesser amount of blood granting that the standard amount anticoagulants will be adjusted and blood collected will be used within 24 hours

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10
Q

Removal of anticoagulants means

A

the procedure is already an open system

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11
Q

Formula in getting the maximum volume of blood to donate

a. in pounds
b. in kg

A

a. Donor’s weight/110 X 450
b. Donor’s weight/50 X 450

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12
Q

Formula in getting the volume of anticoagulant needed to suspend collected blood

A

max. volume of blood to donate/100 X 14

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13
Q

Formula in getting the amount of anticoagulant to be removed from the primary bag

A

63 mL - (volume of needed anticoagulant)

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14
Q

Blood Pressure Determination:

  1. How should the donor be seated?
  2. This is placed on the donor’s arm
  3. An artery being located where the chest piece of the stethoscope is placed to hear beating sounds
  4. The BP apparatus bulb should be squeezed until ______________.
  5. Represents systolic pressure
  6. Represents diastolic pressure
  7. The BP in _______ should be recorded on the Blood Donor Interview Data Sheet.
A
  1. Seated comfortably
  2. Inflatable cuff
  3. Brachial artery
  4. The pressure is enough for you not to hear any beating sounds
  5. First loud sound to reappear (after slowly releasing pressure)
  6. Last sound before it ceases.
  7. mmHg
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15
Q

Allowable limit of:

a. systolic blood pressure
b. diastolic blood pressure

A

a. ≤ 180 mmHg
b. ≤ 100 mmHg

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16
Q

If the inital blood pressure is high and the donor is not known to be hypertensive, what should be the course of action?

If the BP is still high, what should be done?

A

Tell the donor to rest for approx. 15-30 minutes then repeat the BP determination.

Reject the donor and refer to a blood bank physician or medical internist.

17
Q

Pulse rate Determination:

a. What artey is located?
b. How minutes should the number of regular beats be counted?
c. Record the pulse rate in ____________ on the Blood Donor Interview Data Sheet

A

a. Radial artery
b. exactly 1 minute (using timer)
c. beats/minute

18
Q

Acceptable pulse rate for a potential donor

A

50-100 beats/minute

19
Q

Course of action when:
a. The pulse rate is higher than acceptable range
b. If it’s still high even after answer in letter (a)

A

a. Allow donor to rest for about 15 minutes and take the PR again
b. Refer donor to a blood bank physician or medical internist

20
Q

Temperature Determination:

a. How many minutes should the thermometer be placed on donor’s armpit?
b. The temperature should be in
c. Donors are advised no to have ___________ while waiting

A

a. 5 minutes
b. oC and oF
c. Coffee or any hot beverages

21
Q

Formulas for:

Fahrenheit
Celsius

A

F= 1.8 (C) + 32
C = (F - 32) / 1.8

22
Q

Standard donor temperature

A

≤ 37.5 C or ≤ 99.5 F

23
Q

Higher temperature indicates

A

active or current infection

24
Q

Hemoglobin Determination:

a. A skin puncture must be performed on what finger?
b. The first drop of blood should be
c. Squeeze the finger until this has formed
d. The drop should fall freely on this solution

A

a. ring finger
b. wiped
c. solid drop
d. copper sulfate solution

25
Q

a. If the drop of blood sinks within 15s, the hemoglobin level is estimated to be _________.

b. If the drop of blood floats or simply suspends, the hemoglobin level is estimated to be __________.

A

a. >12.5 g/dL
b. <12.5 g/dL

26
Q

Hematocrit Determination:

a. Blood should be collected using this tube until 3/4 filled by using the same puncture site from hemoglobin determination

b. One end should be sealed with _______ and the content should be permanently secured wtih ___________.

c. The capillary tube should be labeled _______.

d. The capillary tube should be spinned in __________ for _____ mins.

e. Used to read packed cell volume

A

a. heparinized capillary tube
b. clay; paraffin wax
c. correctly
d. microhematrocrit centrifuge; 5 minutes
e. microhematocrit reader

27
Q

Normal hemoglobin level; and
Hematocrit level for allogeneic donation

What may result to a level less than the allowable value?

A

≥ 12.5 g/dL;
≥ 38 %

Deferral (postponement of action)

28
Q

Blood type determination:

a. Site
b. How many portions will a glass slide be divided into? Labels?
c. What should be delivered to the respected labels in answer (b)
d. The sampple and antiserum should be mixed with

A

a. same with hemoglobin and hematocrit puncture site
b. 3; A, B, and Rh
c. Anti-A, Anti-B, Anti-D
d. Different applicator sticks

29
Q

Screening of blood donors will not proceed to the next series of tests for blood transmissble diseases if …

why?

A

If the donors failed the preliminary test procedures. This is to save on the blood bank screening reagents

30
Q

Tests for the Blood Transmissible Disease:

MALARIAL PARASITE DETERMINATION

a. Gold standard for malarial parasite diagnosis
b. Smear prepared for blood donor screening
c. Blood may be from _______ or _________
d. Stain
e. Objective used for examination of stained slide

A

a. thin and thick smear
b. thin smear
c. skin puncture (Hgb/ HCT determination) or EDTA blood (veni)
d. Giemsa
e. Oil immersion objective (OIO)

31
Q

Tests for the Blood Transmissible Disease:

SEROLOGICAL TESTS OF OTHER TRANSMISSIBLE DISEASE

a. What tube is is to be used?
b. Blood should be allowed to ______.
c. Centrifuge for _____ mins. at ______ rpm
d. This should be separated and tested for the presence of HIV, HCV, HBsAg, and T. pallidum infection
e. Reported as ________ or _________

A

a. red top tube
b. clot
c. 3 mins, 3400 rpm
d. serum
e. reactive or nonreactive

32
Q

All results should be recorded on

A

Blood Donor Interview Data Sheet

33
Q

First step for most tests

A

Ask donor to sit comfortable on a chair