BB Lab (Blood Donor Screening)

Question 1

Required by every hospital in the Philippines equipped with Blood Banking services.

Answer 1

standard protocol on screening blood donors

Question 2

The standard protocol on screening blood donors ia based on the provisions provided by the national voluntary blood services program duly instituted by the ___________________________.

Answer 2

Department of Health

Question 3

Why is blood donor screening done?

Answer 3

To avoid the risk of blood transmissible diseases by careful examination of the potential donors.

Question 4

Laboratory screening includes: (9)

Answer 4

Weight Determination
Blood Pressure Determination
Pulse Rate Determination
Temperature Determination
Hemoglobin Determination
Hematocrit Determination
Blood Type Determination
Tests for the Blood Transmissible Disease
Bleeding Demonstration

Question 5

Weight Determination:

1. These should be removed as it may add to the standard weight requirement for a donor.
2. Position of the donor on a weighing scale
3. This should be determined
4. The weight should be recorded in what units? On what?

Answer 5

1. Shoes, heavy clothing, apparel
2. Standing straight
3. Weight
4. Pounds and kilograms on Blood Donor Interview Data Sheet

Question 6

1 kg is equivalent to ______ pounds

Answer 6

2.2

Question 7

This tells you the maximum amount of blood that can be drawn from a donor.

Answer 7

weight

Question 8

The maximum allowble blood is suspended in _______________ present in the primary blood bag.

Answer 8

63 mL of anticoagulant

Question 9

If the weight is <110 pounds or <50 kilograms, the blood bank may …

Answer 9

collect lesser amount of blood granting that the standard amount anticoagulants will be adjusted and blood collected will be used within 24 hours

Question 10

Removal of anticoagulants means

Answer 10

the procedure is already an open system

Question 11

Formula in getting the maximum volume of blood to donate

a. in pounds
b. in kg

Answer 11

a. Donor’s weight/110 X 450
b. Donor’s weight/50 X 450

Question 12

Formula in getting the volume of anticoagulant needed to suspend collected blood

Answer 12

max. volume of blood to donate/100 X 14

Question 13

Formula in getting the amount of anticoagulant to be removed from the primary bag

Answer 13

63 mL - (volume of needed anticoagulant)

Question 14

Blood Pressure Determination:

1. How should the donor be seated?
2. This is placed on the donor’s arm
3. An artery being located where the chest piece of the stethoscope is placed to hear beating sounds
4. The BP apparatus bulb should be squeezed until ______________.
5. Represents systolic pressure
6. Represents diastolic pressure
7. The BP in _______ should be recorded on the Blood Donor Interview Data Sheet.

Answer 14

1. Seated comfortably
2. Inflatable cuff
3. Brachial artery
4. The pressure is enough for you not to hear any beating sounds
5. First loud sound to reappear (after slowly releasing pressure)
6. Last sound before it ceases.
7. mmHg

Question 15

Allowable limit of:

a. systolic blood pressure
b. diastolic blood pressure

Answer 15

a. ≤ 180 mmHg
b. ≤ 100 mmHg

Question 16

If the inital blood pressure is high and the donor is not known to be hypertensive, what should be the course of action?

If the BP is still high, what should be done?

Answer 16

Tell the donor to rest for approx. 15-30 minutes then repeat the BP determination.

Reject the donor and refer to a blood bank physician or medical internist.

Question 17

Pulse rate Determination:

a. What artey is located?
b. How minutes should the number of regular beats be counted?
c. Record the pulse rate in ____________ on the Blood Donor Interview Data Sheet

Answer 17

a. Radial artery
b. exactly 1 minute (using timer)
c. beats/minute

Question 18

Acceptable pulse rate for a potential donor

Answer 18

50-100 beats/minute

Question 19

Course of action when:
a. The pulse rate is higher than acceptable range
b. If it’s still high even after answer in letter (a)

Answer 19

a. Allow donor to rest for about 15 minutes and take the PR again
b. Refer donor to a blood bank physician or medical internist

Question 20

Temperature Determination:

a. How many minutes should the thermometer be placed on donor’s armpit?
b. The temperature should be in
c. Donors are advised no to have ___________ while waiting

Answer 20

a. 5 minutes
b. oC and oF
c. Coffee or any hot beverages

Question 21

Formulas for:

Fahrenheit
Celsius

Answer 21

F= 1.8 (C) + 32
C = (F - 32) / 1.8

Question 22

Standard donor temperature

Answer 22

≤ 37.5 C or ≤ 99.5 F

Question 23

Higher temperature indicates

Answer 23

active or current infection

Question 24

Hemoglobin Determination:

a. A skin puncture must be performed on what finger?
b. The first drop of blood should be
c. Squeeze the finger until this has formed
d. The drop should fall freely on this solution

Answer 24

a. ring finger
b. wiped
c. solid drop
d. copper sulfate solution

Question 25

a. If the drop of blood sinks within 15s, the hemoglobin level is estimated to be _________. b. If the drop of blood floats or simply suspends, the hemoglobin level is estimated to be __________.

Answer 25

a. >12.5 g/dL b. <12.5 g/dL

Question 26

Hematocrit Determination: a. Blood should be collected using this tube until 3/4 filled by using the same puncture site from hemoglobin determination b. One end should be sealed with _______ and the content should be permanently secured wtih ___________. c. The capillary tube should be labeled _______. d. The capillary tube should be spinned in __________ for _____ mins. e. Used to read packed cell volume

Answer 26

a. heparinized capillary tube b. clay; paraffin wax c. correctly d. microhematrocrit centrifuge; 5 minutes e. microhematocrit reader

Question 27

Normal hemoglobin level; and Hematocrit level for allogeneic donation What may result to a level less than the allowable value?

Answer 27

≥ 12.5 g/dL; ≥ 38 % Deferral (postponement of action)

Question 28

Blood type determination: a. Site b. How many portions will a glass slide be divided into? Labels? c. What should be delivered to the respected labels in answer (b) d. The sampple and antiserum should be mixed with

Answer 28

a. same with hemoglobin and hematocrit puncture site b. 3; A, B, and Rh c. Anti-A, Anti-B, Anti-D d. Different applicator sticks

Question 29

Screening of blood donors will not proceed to the next series of tests for blood transmissble diseases if ... why?

Answer 29

If the donors failed the preliminary test procedures. This is to save on the blood bank screening reagents

Question 30

Tests for the Blood Transmissible Disease: MALARIAL PARASITE DETERMINATION a. Gold standard for malarial parasite diagnosis b. Smear prepared for blood donor screening c. Blood may be from _______ or _________ d. Stain e. Objective used for examination of stained slide

Answer 30

a. thin and thick smear b. thin smear c. skin puncture (Hgb/ HCT determination) or EDTA blood (veni) d. Giemsa e. Oil immersion objective (OIO)

Question 31

Tests for the Blood Transmissible Disease: SEROLOGICAL TESTS OF OTHER TRANSMISSIBLE DISEASE a. What tube is is to be used? b. Blood should be allowed to ______. c. Centrifuge for _____ mins. at ______ rpm d. This should be separated and tested for the presence of HIV, HCV, HBsAg, and T. pallidum infection e. Reported as ________ or _________

Answer 31

a. red top tube b. clot c. 3 mins, 3400 rpm d. serum e. reactive or nonreactive

Question 32

All results should be recorded on

Answer 32

Blood Donor Interview Data Sheet

Question 33

First step for most tests

Answer 33

Ask donor to sit comfortable on a chair