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SECOND SEM 3rd YEAR > MOL BIO LAB L5 (Midterms- Lab Set-up) > Flashcards

MOL BIO LAB L5 (Midterms- Lab Set-up) Flashcards

1
Q

The laboratory should be compatible with ________ to prevent contamination

A

mechanical barrier

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2
Q

This is the most common issue in the molecular laboratory

A

contamination

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3
Q

What area does the sample preparation room belong to?

A

Area 1

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4
Q

What area does the Amplification room belong to?

A

Area 3

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5
Q

What area does the Reagent Preparation room belong to?

A

Area 2

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6
Q

An amplification room/area where the gel electrophoresis room is located

A

Analysis of PCR Products

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7
Q

serves as a clean bench area

A

Dead airbox with UV light

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8
Q

An amplification room/area where the PCR room is located

A

PCR amplification

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9
Q

Room/ area in the molecular biology lab where biosafety cabinet (optional) is located

A

Nucleic acid Extraction Room/ Area

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10
Q

The only room/area in the molecular biology lab where there is positive air pressure

A

Reagent preparation room/area

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11
Q

Air pressure in Sample prep and Post amplification rooms/areas

A

Negative air pressure

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12
Q

Single entrance, reagents used for amplification should not be exposed to other areas

A

Reagent prep

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13
Q

Specimens should not be exposed to post-amplification work areas

A

Specimen prep

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14
Q

The size of each area should consider ________ for equipment and ________ needed for preparation

A

space; bench space

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15
Q

Alternative to Spatial Separation

A
  • Class II biological safety cabinet
  • Dedicated areas for each work phase
  • Unidirectional
  • Automated specimen processing station/closed tube amplification and detection system
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15
Q

State the other lab design considerations (at least three)

A
  • Temperature and humidity requirements
  • Exhaust ventilation
  • Water quality
  • Electric outlet
  • Back-up power system
  • Eyewash
  • Ergonomic assessment
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16
Q

This is always included when checking for contamiantion

A

blank (no template) control

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16
Q

What is to be avoided when pipetting?

A

Production of aerosols

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16
Q

The PCR workstation is cleaned at every?

A

Start and end of the workday/run

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17
Q

The PCR workstation is cleaned using?

A

Uv light, 70% ethanol, fresh 10% sodium hypochlorite, DNA Away

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18
Q

This induces thymidine dimers and other modifications that render nucleic acid inactive as a template for amplification

A

Ultra-violet light irradiation

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18
Q

Work stations should be cleaned with __________, followed by removal with _________

A

10% sodium hypochlorite solution (bleach; ethanol and water

19
Q

Substitution of uracil (dUTP) for thymine (dTTP) during PCR amplification

A

Enzymatic inactivation with uracil-N-glycosylase

20
Q

Contaminating PCR amplicons are degraded leaving only genomic DNA avaialble for PCR

A

New PCR sample reactions pre-treated with Uracil-N-glycosylase (UNG)

21
Q

Wipe test (swab test) is performed?

A

Monthly

22
Q

This is used to detect, localize, and remove contamination and identify the source of the contamination

A

Wipe test (swab test)

22
Q

Proficiency testing is performed?

A

Twice a year

23
Q

Used for assessment of the Competence in Testing

A

Proficiency Testing

24
Q

If specimens are not commercially available alternative proficiency testing program has to be?

A

established (specimen exchange etc.)

25
Q

Addresses emergencies from an all-hazards
approach. This establishes policy-and guidance ensuring that critical functions continue and that personnel and resources are
relocated to an alternate facility in case of emergencies.

A

Continuity of Operations Plan (COOP Plan)

26
Q

Uses of molecular laboratory in the field of clinical diagnostics

A
  1. Health
  2. Medicine
  3. Forensics
  4. Pharmaceutical Industry
  5. Biological warfare
  6. Drug Discovery
  7. PCR based Technology
  8. Fluorescence in situ Hybridization (FISH)
  9. Biochip
  10. Peptide Nuicleic acid (PNA)
    11.Proteomic Technology :
  11. Electrochemical Detection of DNA
27
Q

Potential sources of contamination are?

A
  • Cross contamination between specimens
  • amplification product contamination
  • laboratory surfaces
  • ventilation ducts
  • reagents/supplies and
  • hair, skin, saliva, and clothes of laboratory personnel.
28
Q

Contamination may cause?

A
  • Incorrect results
  • Require extensive-cleanup
  • Loss of creditability
  • Impact on financial and performance
29
Q

Contamination can be controlled using proper?

A
  • Laboratory-design
  • Laboratory practices
  • Chemical and enzymatic controls
30
Q

Nucleic acids used be stored/ used in what temp?

A

4-8°C or -15 to -25°C

31
Q

A fundamental skill in molecular biology, as it ensures that reagents
and samples are measured accurately and reliably

A

Precise pipetting

32
Q

These pipettes are used for handling small volumes, typically in
the microliter range

A

Micropipette

33
Q

They are essential for PCR, DNA quantification, and sample transfers.

A

Micropipette

34
Q

These are used for transferring larger-volumes, typically in
the milliliter range. Often used for media preparation, cell
culture, and larger-scale molecular biology experiments.

A

Serological pipettes

34
Q

Multichannel pipettes have multiple channels, usually _______, that allow for simultaneous pipetting of multiple samples. They are commonly used in high-throughput applications.

A

8 or 12

35
Q

Two commonly used techniques in pipetting

A

Forward and reverse

36
Q

The more traditional-and widely used technique

A

Forward pipetting

37
Q

Effective when working with viscous or volatile samples

A

Reverse pipetting

37
Q

This technique reduces the risk of over-pipetting and ensures the intended volume is accurately delivered

A

Reverse pipetting

38
Q

Suitable for general sample transfers and dilutions

A

Forward pipetting

38
Q

This technique is commonly used when precise volume measurements are required, such as in quantitative assays where even minor deviations can affect results

A

Forward pipetting

39
Q

This technique is ideal for transferring sample with unique characteristics, like highly viscous substances or volatile solutions

A

Reverse pipetting

40
Q

Often used in the preparation of high-concentration reagents and for DNA/RNA extractions

A

Reverse pipetting

41
Q

Essential to maintain the accuracy and reproducibility of experiments.

A

pH measurement

41
Q

Measures the hydrogen ion concentration in a solution

A

pH

42
Q

Formula to calculate dilutions

A

(Concentration stock) X (volume of stock used) = (Concentration final) X (final volume)

43
Q

Monitor all steps of analytical procedure

A

Quality Control Plan

44
Q

Measurement to monitor and record specific activities as part of the quality

A

Quality Indicator
- Turn around time
- % of failed runs
- Population medium
- Calibrator parameters
- Graph to identify trend or shift
- Monitor frequency and acceptable range

SECOND SEM 3rd YEAR (38 decks)

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