Bordetella pertussis, Haemophilus influenzae, Legionella pneumophila Flashcards Preview

Micro Exam #2 > Bordetella pertussis, Haemophilus influenzae, Legionella pneumophila > Flashcards

Flashcards in Bordetella pertussis, Haemophilus influenzae, Legionella pneumophila Deck (77)
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1

Describe the structure of Bordetella pertussis.

Small, G- Coccobacillus
Encapsulated
Non-motile

2

Bordetella pertussis is associated with _______ in virulent strains.

Hemolysis

3

Incubation time of Bordetella pertussis?

1-2 weeks

4

Three stages of Bordetella pertussis?

Catarrhal stage
Paroxysmal Stage
Convalescent Stage

5

Describe symptoms in the catarrhal stage of Bordetella pertussis infection.

Low grade fever, runny nose, worsening cough

6

Describe the clinical presentation of the paroxysmal stage of Bordetella pertussis infection.

Severe Cough -- So prolonged/irritated that inhalazion is wheezy and vomiting occurs

Lymphotoxicity results in leukocyte prolif

7

Common name of Bordetella pertussis?

Whooping Cough

8

Describe the Bordetella pertussis convalescent stage.

Cough becomes less severe for about 45 days
"The Cough of 100 days"

9

Complications of Bordetella pertussis?

Bronchopneumonia
Encephalitis

10

How is Bordetella pertussis spread?

Respiratory droplets

11

Why is Bordetella pertussis so prone to spread?

It is highly contagious
It is rarely detected before spread

12

In what stage is Bordetella pertussis most contagious?

Catarrhal stage

13

Who is most prone to get Bordetella pertussis? Who is it most dangerous in?

Small Children
Most dangerous in small airway

14

Four causes of Bordetella pertussis?

Increased Surveillance
Increased Toxin Production
Vaccine targets few antigens
Low coverage of booster vaccine

15

Six virulence factors of Bordetella pertussis

Filamentus hemagglutinin
Pertussis toxin
Calmodulin-dependent adenyl cyclase
Dermonecrotic Toxin
Tracheal Cytotoxin
LPS

16

What is the activity of filamentous hemagglutinin?

- Allows Bordetella pertussis to bind specifically to glycoprotein receptor on ciliated epithelial cells
- Aided by pertactin surface protein, pili, pertussis toxin

17

What is the clinical significance of filamentous hemagglutinin?

Ciliary stasis of mucociliary escalator
(can't sweep bacteria out of resp. tract)

18

Describe the six parts of pertussis toxin.

5 B parts bind to ganglioside for ciliated cells
A part - ADP ribosylates an inhibitory G ptorein

19

What does ribosylating the inhibitory G protein in Bordetella pertussis cause problems

Prevents inactivation of adenyl cyclase (inc. cAMP)
Increases H2O secretion in respiratory tract

20

Difference between pertussis toxin and cholera toxin.

Pertussis locks in stimulated/active form
Cholera blocks the inhibitory form

21

Effect of calmodulin-dependent adenyl cyclase.

Additional ACase, only active in eukaryotic cells

22

Role of dermonecrotic toxin? Who secretes it?

Role Uncertain
Secreted by T3SS

23

What is tracheal cytotoxin? What does it do?

Soluble peptidoglycan residue
Kills ciliated epithelial cells

24

What two lipids are contained in LPS?

Lipid A and Lipid X (half of A)

25

How is Bordetella pertussis controlled?

DTap Vaccine 3 times before 1st birthday, boosters

26

How can Bordetella pertussis be treated? What stage must a patient be in?

Erythromycin/Azithromycin
Catarrhal Stage

27

Describe Bordetella parapertussis

Similar to whooping cough, less severe
Have cryptic ptx operon

28

Describe the physical structure ofHaemophilus influenzae

G- coccobacillus
Some have capsule

29

Difference between typable and non-typable Haemophilus influenzae?

Typable have capsule, non-typable do not

30

Why to culture and make apparent Haemophilus influenzae?

Capsulated forms irridescent on BHI agar