Flashcards in Listeria, Pseudomonas, Various Anerobes Deck (38)
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1
Describe the physical structure of Listeria monocytogenes
Thinnish G+ Rods
Hemolysis on Blood Agar
2
Listeria monocytogenes temperature preference?
Psychrophile
3
Listeria monocytogenes is motile at ________
Diptheroids is motile at ________
Room Temp, Not 37C
Not room temp, 37C
4
Listeria monocytogenes infection occurs where molecularly?
intracellular
5
Clinical presentation on Listeria monocytogenes
Native Flora in Animals
Infection following ingestion of infected food
Diarrhea, Dysentery, Meningitis, Bacteremia`
6
Who gets Listeria monocytogenes?
IC and Infants
Also, 2 perinatal versions
7
Clinical presentation of early onset perinatal Listeria monocytogenes
Stillbirth with granulomas
8
Clinical presentation of late onset perinatal Listeria monocytogenes
Infant bacterial meningitis
9
How is Listeria monocytogenes typically contracted?
Contaminated Food
Long term refrigerator storage of meats and cheese
10
Pathogenesis of Listeria monocytogenes?
Siderophores that bind Fe
Intracellular -- uptake via Internalin
low pH of lyso activated listerilysin O
Break out of lysosome
ActA causes actin to propel Listeria to other cells
11
Name two other bacterium that use Act A mechanism
Rikketsia and Shigella
12
How is Listeria monocytogenes treated?
Ampicillin preferred
Erythromycin will do in a pinch
No Vaccine because intracellular
13
Physical structure of pseudomonas aeruginosa
G- Rod with Single Polar Flagellum
Robust Metabolic Capacity
Fruity Aroma
Blue-green fluorescent pigments
14
Metabolism of pseudomonas aeruginosa?
Respiratory, but can use anerobic with NO3
15
Pseudomonas aeruginosa quorum sensing leads to....
Biofilm Formation
16
Clinical Presentation of Pseudomonas aeruginosa?
Opportunistic, Nosocomial Infections
Skin Biofilms
CF Patients
Otitis Externa
Eye Infections
UTI
Bacteremia/Sepsis (Leukemia Patients)
Ecthyma gangrenosum
Folliculitis
17
Who is most prone to develop Pseudomonas aeruginosa biofilms?
Burn Patients, Diabetics
18
What is ecthyma gangrenosum?
Erythematous, Non-pyogenic ulcerations
19
Two sources of Pseudomonas aeruginosa folliculitis?
Contaminated Water (Hot Tub)
Acne
20
T or F. Pseudomonas aeruginosa is typically foundin the URT flora
F. Only in CF patients
21
Pathogenesis of Pseudomonas aeruginosa?
Adhesion pili
Hypermutable and Persister Strains
Biofilms deter Phagocytosis
LPs endotoxin
Type 3 secretion system secretes toxins
22
Toxins secreted by Pseudomonas aeruginosa
Exotoxin A and S -- ADP ribosylation (s sigs apop)
Elastase -- Destroy Elastins in the Lungs
Pyocyanin/Pyochelin -- Make toxic O2 radicals
23
How is Pseudomonas aeruginosa controlled?
Sterile precautions with burn patients
Topical antimicrobials on wounds
Prevent BF formation
24
Best therapy usually for Pseudomonas aeruginosa?
Cipro + Aztreonam
25
What should you do before long-term Pseudomonas aeruginosa therapy?
Assess resistance profile
26
Significance of Acinetobacter baumanii?
Nosocomial infections
Ventilator associated pneumonia, septicemia
Highly Resistant
27
Significance of Burkholderia cepacia?
Similar to Pseudomonas aeruginosa
Catheter associated UTI
28
Anerobes typically have a _____ redox potential.
Negative
29
How are the low-redox potential regions preferred by anerobes typically maintained?
Facultative organisms like E. Coli
30
Physical structure of Bacteroides fragilis
G- Rod of the Colon
31
Virulence factors of Bacteroides fragilis?
Antiphagocytic Capsule
SOD and CAT (Not as badly affected by O2)
Extracellular Enzymes (Neurominidase, Heparinase)
32
Significance of Prevotella melaninogenicia?
Oral G- Coccobacillis
VF: Collagenase
33
Significance of Porphyromonas gingivalis?
Oral G-Rod
Gingivitis, Oral Abscess
Also infects other warm, moist areas
34
Significance of Fusobacterium?
Oral and Colonic
G- Fusiform
Infectious in osteomyelitis
35
Significance of Peptostreptococcus?
Colonic G+ Coccus
Infection of Blood and Pleura/Lungs
36
Significance of Propionibacterium acnes?
Epidermal G+ Pleomorphic Rod
Acne and Brain Abscesses
37
Pathogenesis of various anerobes?
Normal flora enter unprotected area via trauma
Production of tissue destructive enzymes causes an abscess
38
