Listeria, Pseudomonas, Various Anerobes Flashcards Preview

Micro Exam #2 > Listeria, Pseudomonas, Various Anerobes > Flashcards

Flashcards in Listeria, Pseudomonas, Various Anerobes Deck (38)
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1

Describe the physical structure of Listeria monocytogenes

Thinnish G+ Rods
Hemolysis on Blood Agar

2

Listeria monocytogenes temperature preference?

Psychrophile

3

Listeria monocytogenes is motile at ________
Diptheroids is motile at ________

Room Temp, Not 37C
Not room temp, 37C

4

Listeria monocytogenes infection occurs where molecularly?

intracellular

5

Clinical presentation on Listeria monocytogenes

Native Flora in Animals
Infection following ingestion of infected food
Diarrhea, Dysentery, Meningitis, Bacteremia`

6

Who gets Listeria monocytogenes?

IC and Infants
Also, 2 perinatal versions

7

Clinical presentation of early onset perinatal Listeria monocytogenes

Stillbirth with granulomas

8

Clinical presentation of late onset perinatal Listeria monocytogenes

Infant bacterial meningitis

9

How is Listeria monocytogenes typically contracted?

Contaminated Food
Long term refrigerator storage of meats and cheese

10

Pathogenesis of Listeria monocytogenes?

Siderophores that bind Fe
Intracellular -- uptake via Internalin
low pH of lyso activated listerilysin O
Break out of lysosome
ActA causes actin to propel Listeria to other cells

11

Name two other bacterium that use Act A mechanism

Rikketsia and Shigella

12

How is Listeria monocytogenes treated?

Ampicillin preferred
Erythromycin will do in a pinch

No Vaccine because intracellular

13

Physical structure of pseudomonas aeruginosa

G- Rod with Single Polar Flagellum
Robust Metabolic Capacity
Fruity Aroma
Blue-green fluorescent pigments

14

Metabolism of pseudomonas aeruginosa?

Respiratory, but can use anerobic with NO3

15

Pseudomonas aeruginosa quorum sensing leads to....

Biofilm Formation

16

Clinical Presentation of Pseudomonas aeruginosa?

Opportunistic, Nosocomial Infections
Skin Biofilms
CF Patients
Otitis Externa
Eye Infections
UTI
Bacteremia/Sepsis (Leukemia Patients)
Ecthyma gangrenosum
Folliculitis

17

Who is most prone to develop Pseudomonas aeruginosa biofilms?

Burn Patients, Diabetics

18

What is ecthyma gangrenosum?

Erythematous, Non-pyogenic ulcerations

19

Two sources of Pseudomonas aeruginosa folliculitis?

Contaminated Water (Hot Tub)
Acne

20

T or F. Pseudomonas aeruginosa is typically foundin the URT flora

F. Only in CF patients

21

Pathogenesis of Pseudomonas aeruginosa?

Adhesion pili
Hypermutable and Persister Strains
Biofilms deter Phagocytosis
LPs endotoxin
Type 3 secretion system secretes toxins

22

Toxins secreted by Pseudomonas aeruginosa

Exotoxin A and S -- ADP ribosylation (s sigs apop)
Elastase -- Destroy Elastins in the Lungs
Pyocyanin/Pyochelin -- Make toxic O2 radicals

23

How is Pseudomonas aeruginosa controlled?

Sterile precautions with burn patients
Topical antimicrobials on wounds
Prevent BF formation

24

Best therapy usually for Pseudomonas aeruginosa?

Cipro + Aztreonam

25

What should you do before long-term Pseudomonas aeruginosa therapy?

Assess resistance profile

26

Significance of Acinetobacter baumanii?

Nosocomial infections
Ventilator associated pneumonia, septicemia
Highly Resistant

27

Significance of Burkholderia cepacia?

Similar to Pseudomonas aeruginosa
Catheter associated UTI

28

Anerobes typically have a _____ redox potential.

Negative

29

How are the low-redox potential regions preferred by anerobes typically maintained?

Facultative organisms like E. Coli

30

Physical structure of Bacteroides fragilis

G- Rod of the Colon