Chapter 3 MICRO Flashcards
(24 cards)
what are the five I’s
inoculation, incubation, isolation, inspection, and identification
describe inoculation
introduce sample of microorganism onto medium
try to create sterile inoculation
describe incubation
medium is placed in temperature controlled incubator
can also control atmospheric gases
microbe multiplies
what three ways can one classify a medium
physical state
chemical composition
functional type
a defined media is
a media whose exact chemical compositions are known
what a complex media
dont know the exact chemical composition
what is an enriched medium
contains complex organic substances such as blood, serum, hemoglobin or special growth factors
bacteria that require growth factors and complex nutrients are termed
fastidious
what is a selective medium
contains one or more agents that inhibit the growth of certain microbe or microbes but not others
dyes, antimicrobial drugs
what is a differential media
allow multiple types of microorganisms to grow but are designed to display visible differences among their colonies
colony size or color, media color changes, gas bubble or precipitates
chemicals, dyes, blood agar
what is a reducing medium
contains a substance that absorbs oxygen or slows the penetration oxygen in a medium
anaerobic bacteria
Trasport media is
used to maintain and preserve specimens that have to be held for a period of time before clinical analysis or to sustain delicate species that die rapidly if not held under stable conditions
Assay media is used for
testing the effectiveness of antimicrobial drugs
Describe isolation
used to grow a microbe and its colony
take small number of cells and spread over media hoping to isolate specific microbe
what is a pure culture
a container of medium that grows only single known species or type of microorganism
what is a mixed culture
container that holds two or more identified, easily differentiated species of microorganisms
describe inspection
view specimen microscopically to observe characteristics
maybe have to stain slide
describe identification
use relevant data to compare microbe to
biochemical tests
label how the lens on microscopes go from light source to eyes
condenser lens, objective lens and ocular lens
what is resolultion
distinguish or separate two adjacent objects or points from one another
differential stains use
a primary dye and counterstain
what are the four types of visible light microscopy
bright field, dark field, phase contrast and interference
transmission electron microscope
transmit electrons through specimen
scanning electron microscope
gives a 3D image of specimen, metal coated specimen
