CHAPTER 8 Flashcards

1
Q

refers to events that occur outside the body,

A

vitro

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2
Q

refers to events that occur inside the body.)

A

vivo

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3
Q

factors that affect microbial growth

A

the availability of nutrients
moisture,
temperature,
pH
osmotic pressure
barometric pressure
composition of the atmosphere.

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4
Q

Cells are composed of
between __% and ___%
water.

A

70 - 95

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5
Q

There are certain microbial stageshowever, that can survive the complete drying process this is called

A

dessication

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6
Q

Microorganisms that grow
best at high temperatures are
called

A

thermophiles

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7
Q

Microbes that grow best at moderate temperatures
are called

A

mesophiles.

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8
Q

This group includes most of the
species that grow on plants and animals and in warm soil and water.

A

mesophiles.

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9
Q

prefer cold
temperatures. They thrive in cold ocean water. At high altitudes, algae (often pink) can be seen living on snow.

A

Psychrophiles

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10
Q

Microorganisms that prefer warmer
temperatures, but can tolerate or endure very cold temperatures and can be preserved in the frozen state, are
known as

A

psychroduric organisms.

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11
Q

Most
microorganisms prefer a neutral or slightly alkaline
growth medium

A

(pH 7.0–7.4),

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12
Q

microbes such as those that can live in the stomach and in pickled foods, prefer a pH of 2 to 5.

A

(acidophiles),

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13
Q

the only
human pathogen that grows well above pH 8.

A

Vibrio
cholerae

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14
Q

is the pres-
sure that is exerted on a cell membrane by solutions both
inside and outside the cell.

A

Osmotic pressure

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15
Q

When the concentration of solutes in the environment outside of a cell is greater than the concentration of solutes inside the cell, the solution in which the cell is suspended is said to be

A

hypertonic.

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16
Q

is defined as the movement of a solvent , through a permeable membrane, from a solution having a lower concentration of solute to a solution hav-
ing a higher concentration of solute.

A

Osmosis

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17
Q

the loss of water causes the cell to shrink; this shrinkage is called

A

crenation

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18
Q

Instead, the cell membrane and cytoplasm shrink away from the cell wall. This condition, known as

A

plasmolysis,

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19
Q

Cells swell up, and
sometimes burst,
when placed into

A

hypotonic solution.

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20
Q

If sufficient water enters, the cell will
burst (lyse). In the case of erythrocytes, this bursting is called

A

hemolysis.

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21
Q

cells having rigid cell walls such as plant cells and bacteria. If the pressure becomes so great
that the cell ruptures, the escape of cytoplasm from the cell is referred to as

A

plasmoptysis.

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22
Q

When the concentration of solutes outside a cell equals the concentration of solutes inside the cell, the so-
lution is said to be

A

isotonic.

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23
Q

Those microbes that actually prefer salty environments are
called

A

halophilic,

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24
Q

Organisms that do not prefer to live in salty environments but are capable of surviving
there

A

haloduric organisms.

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25
known as ___, thrive deep in the ocean and in oil wells, where the atmospheric pressure is very high.
piezophiles
26
The media (sing., medium) that are used in microbiology laboratories to culture bacteria are referred to as
artificial media or synthetic media,
27
medium is one in which all the ingredients are known; this is because the medium was prepared in the laboratory by adding a certain number of grams of each of the components
chemically defined
28
medium is one in which the exact contents are not known. Complex media contain ground up or digested extracts from animal organs (e.g., hearts, liv- ers, brains), fish, yeasts, and plants, which provide the necessary nutrients, vitamins, and minerals.
complex media
29
The time it takes for one cell to become two cells by binary fission is called the
gen- eration time.
30
Bacteria multiply by
binary fission.
31
is a broth or solid medium containing a rich supply of special nutrients that promotes the growth of fastidious organisms. It is usually prepared by adding extra nutrients to a medium called nutrient agar.
enriched medium
32
is practiced in the microbiology laboratory to prevent infection of individuals and contamination of the work environment, clinical specimens, and cultures.
Aseptic technique
33
A bacterial population growth curve consists of four phases:
Lag Log Stationary Death
34
during which the bacteria absorb nutrients, synthesize enzymes, and prepare for cell division.
lag phase
35
what phase wherethe bacteria multiply so rapidly that the number of organisms doubles with each generation time
Log phase
36
The bacteria do not increase in number during the
Lag phase
37
It is during this phase that the culture is at its greatest population density.
Stationary phase
38
is used to determine the number of viable bacteria in a liquid sample, such as milk, water, ground food diluted in water, or a broth culture.
viable plate count
39
describes the elimination of most or all pathogens (except bacterial spores) from nonliving objects.
Disinfection
40
involves the destruction or elimination of all microbes,
Sterilization
41
is a drug or chemical that inhibits reproduction of microorganisms, but does not necessarily kill them.
microbiostatic agent
42
one that specifically inhibits the metabolism and reproduction of bacteria.
bacteriostatic agent
43
is a process that combines dehydration (dry- ing) and freezing.
Lyophilization
44
the most practical, efficient, and inexpensive method of sterilization of those inanimate objects and materials that can withstand high tempera- tures.
Heat
45
refers to the presence of pathogens in blood or tissues,
sepsis
46
is the prevention of infection.
antisepsis
47
any particular species of microorganism is the lowest temperature that will kill all the organisms in a standardized pure culture within a specified period.
thermal death point (TDP)
48
is the length of time necessary to sterilize a pure culture at a specified temperature.
thermal death time (TDT)
49
an effective means of destroying contaminated disposable materials.
Incineration
50
is like a large metal pressure cooker that uses steam under pressure to completely destroy all microbial life
autoclave
51
cleaners consist of tanks filled with liquid solvent (usually water); the short sound waves are then passed through the liquid.
Ultrasonic Waves
52
Antimicrobial chemical agents that can safely be applied to skin
Antiseptics
53
1. It would be necessary to use a tuberculocidal agent to kill a particular species of: a. Clostridium. b. Mycobacterium. c. Staphylococcus. d. Streptococcus.
b
54
Pasteurization is an example of what kind of technique? a. antiseptic b. disinfection c. sterilization d. surgical aseptic
b
55
The combination of freezing and drying is known as: a. desiccation. b. lyophilization. c. pasteurization. d. tyndallization.
b
56
Organisms that live in and around hydrothermal vents at the bottom of the ocean are: a. acidophilic, psychrophilic, and halophilic. b. halophilic, alkaliphilic, and psychrophilic. c. halophilic, psychrophilic, and piezophilic. d. halophilic, thermophilic, and piezophilic.
d
57
When placed into a hypertonic solution, a bacterial cell will: a. take in more water than it releases. b. lyse. c. shrink. d. swell.
c
58
To prevent Clostridium infections in a hospital setting, what kind of disinfectant should be used? a. fungicidal b. pseudomonicidal c. sporicidal d. tuberculocidal
c
59
Sterilization can be accomplished by use of: a. an autoclave. b. antiseptics. c. medical aseptic techniques. d. pasteurization.
a
60
The goal of medical asepsis is to kill __________, whereas the goal of surgical asepsis is to kill __________. a. all microorganisms . . . . . pathogens b. bacteria . . . . . bacteria and viruses c. nonpathogens . . . . . pathogens d. pathogens . . . . . all microorganisms
d
61
Which of the following types of culture media is selective and differential? a. blood agar b. MacConkey agar c. phenylethyl alcohol agar d. Thayer-Martin agar
b
62
All the following types of culture media are enriched and selective except: a. blood agar. b. colistin–nalidixic acid agar. c. phenylethyl alcohol agar. d. Thayer-Martin agar.
a