Chapter 9 Flashcards

(43 cards)

1
Q

Transduction

A

The process where a bacteriophage serves as the DNA carrier from a donor cell to a recipient cell

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2
Q

Generalized transduction

A

Random fragments of disintegrating host DNA are taken up by the phage during assembly

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3
Q

Transposons

A

Transposable elements that shift from one part of the genome to another and are termed “jumping genes”

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4
Q

Transformation

A

Nonspecific acceptance by a bacterial cell of small fragments of soluble DNA from the surrounding environment; facilitated by special DNA-binding proteins on the cell wall that capture DNA from the surrounding medium

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5
Q

Competent

A

Ella that are capable of accepting genetic material through transformation

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6
Q

Redundancy or degeneracy

A

A particular amino acid can be coded for by more than one codon

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7
Q

Wobble property

A

Thought to permit some variation or mutation without changing the message

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8
Q

Start codon

A

AUG

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9
Q

Nonsense or stop codons

A

Carry a necessary message instructing the enzyme to stop at a specific place

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10
Q

Polyribosomal complex

A

An assembly line for mass production of proteins

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11
Q

Introns

A

Do not code for proteins; several intervening sequences of triplets

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12
Q

Exons

A

Translated into proteins; introns that are interspersed between coding regions

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13
Q

Operons

A

A section of DNA that contains one or more structural genes along with a corresponding operator gene that controls transcription

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14
Q

Inducible operons

A

The operon is turned on (induced) by the substrate of the enzyme for which the structural gene codes

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15
Q

Repressible operons

A

Often contain genes codon for anabolic enzymes such as those used to synthesize amino acids

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16
Q

Lactose (lac) operon

A

A system used to explain control through genetic induction; accounts for the regulation of lactose metabolism

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17
Q

Regulator

A

Composed of the gene that coded for a protein capable of repressing the operon (a repressor)

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18
Q

Operator

A

A sequence that acts as an on/off switch for transcription

19
Q

Allosteric

A

It’s shape can change depending on which active site and substrate are in play

20
Q

Inducer

A

Responsible for stimulating the chain of events leading to protein synthesis

Ex: lactose

21
Q

Corepressor

A

Slows the transcription of the operon

22
Q

Mutation

A

A phenotypic change due to an alteration in the genotype

23
Q

Wild type or wild strain

A

A microorganism that exhibits a natural, non mutated characteristic

24
Q

Mutant strain

A

A microorganism that bears a mutation; vary in morphology, nutritional characteristics, genetic control mechanisms, resistance to chemicals, temperature preference, and nearly any type of enzymatic function

25
Spontaneous mutation
A random change in the DNA arising from errors in replication that occur without a known cause
26
Induced mutations
Result from exposure to mutagens
27
Mutagens
Physical or chemical agents that damage DNA and interfere with its functioning
28
Point mutations
Latter mutations that involve addition, deletion, or substitution of single based
29
Missense mutation
Any change in the code that leads to placement of a different amino acid; can do one of these: 1. Create a faulty, nonfunctional protein 2. Produce a protein that functions in a different manner 3. Cause no significant alteration
30
Nonsense mutation
Changes a normal codon into a stop codon that doesn't code for an amino acid and stops the production of the protein wherever it occurs
31
Silent mutation
Alters a base but does not change the amino acid and has no effect
32
Back-mutation
Occurs when a gene that has undergone mutation reverses (mutates back) to its original base composition
33
Frameshift
The reading frame of the mRNA has been changed; nearly always result in the addition of deletion of amino acids
34
The Ames test
A screening system to detect chemicals with carcinogenic potential
35
Recombination
One bacterium donates DNA to another bacterium is a type of genetic transfer; resulting in a new strain different from the donor and original strains
36
Recombinant
Any organism that has acquired genes that originated in another organism
37
Plasmids
Small chromosomal fragments; circular pieces of DNA that contain their own origin of replication and therefore can replicate independently of the bacterial chromosome
38
Conjugation
Requires the attachment of two cells and the formation of a bridge that can transport DNA
39
Transformation
The transfer of naked DNA and requires no special vehicle
40
Transduction
DNA transfer mediated through the action of a bacterial virus
41
Fertility or F factor
Only gram negative cells that that operate with a specific plasmid
42
Pilus or sex pilus
Functions in most conjugation transfers
43
Resistance (R) plasmids, or factors
Bear genes for resisting antibiotics and other drugs