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Microbiology > Laboratory Exam > Flashcards

Laboratory Exam Flashcards

(58 cards)

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1
Q

Gram stain controls

A

Positive control: S. epi

Negative control: E. coli

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2
Q

Acid fast stain controls

A

Positive control: M. smeg

Negative control: S. epi

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3
Q

Endospore stain controls

A

Positive control: B. sub

Negative control: E. coli

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4
Q

Gram Negative Bacilli bacteria ID tests

A

Gram stain, MacConkeys, (MACs), Indole, Citrate, TSI

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5
Q

Gram Positive Cocci bacteria ID tests

A

Gram stain, TSY plate,

mannitol salt agar (MSA), Bacitracin

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6
Q

Gram Positive Bacilli bacteria ID tests

A

Gram stain
Acid fast stain
TSI
Endospore stain

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7
Q

Mannitol salt streak (MSA)

A

Streak a line down the middle and then going the same way, inoculate each half separately

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8
Q

MacConkeys streak plate (MACs)

A

Streak a line down the middle then turn the plate halfway and inoculate the entire plate across

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9
Q

Blood agar streak plate

A

Do a lazy river streak in your quadrant from the outer edge of the plate to the middle

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10
Q

TSY streak plate

A

Four quadrant inoculation; touch twice, touch twice, touch once

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11
Q

Bacitracin streak plate

A

Streak a line down the middle of the plate then inoculate the entire plate without turning it

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Z

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12
Q

Penicillin MOA

A

Inhibits cell wall synthesis

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13
Q

Erythromycin MOA

A

Inhibits bacterial protein synthesis by binding to 50s ribosomal subunits

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14
Q

Ciprofloxin MOA

A

Inhibition of enzymes required for DNA replication, transcription, repair, and recombination

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15
Q

Tetracycline MOA

A

Inhibits protein synthesis

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16
Q

Oxacillin MOA

A

Inhibits cell wall synthesis

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17
Q

Sulfodiazine MOA

A

Inhibited by pus; needed for folic acid synthesis

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18
Q

Gram positive results

A

Purple

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19
Q

Gram negative results

A

Pink

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20
Q

Acid-fast results

A

Pink/ red

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21
Q

Nonacid-fast results

A

Purple/ blue

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22
Q

Endospore forming results

A

Green

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23
Q

Nonendospore forming (vegetative) results

A

Pink

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24
Q

TSY results

Study These Flashcards
A

Observe and record colony morphology

25
MacConkeys (MACs)
Is there growth? If it does, determine if it is a lactose fermenter or not. Deep pink colony color (positive) is a lactose fermenter; if not its negative
26
Mannitol salt agar (MSA)
Is there growth? If there is determine if it fermented the mannitol or not. Mannitol fermenter will turn the media yellow (positive); if not its negative
27
Blood agar
Record colony morphology and hemolysis pattern. Classify as alpha, beta, or gamma
28
Bacitracin disc
Measure the zone of inhibition in millimeters Zones > 30mm are sensitive to the antibiotic (positive) Zones < 30mm are resistant to the antibiotic (negative)
29
Indole
Red/pink ring formed at the top of the tube indicates a positive test; no ring-negative test
30
Methyl red
Pink/ red color is positive; no color change is negative
31
Voges-Proskauer
Red color- positive No color change- negative
32
Citrate
Is there growth in the slant? Record the color. Blue color- positive result Green color or no change- negative result
33
TSI Glucose only
Pink slant (alkaline) over yellow butt (acid)=glucose was fermented Recorded as K/A meaning only glucose was fermented
34
TSI Glucose, Lactose, and Sucrose
Slant and butt are yellow (acid) = A/A meaning all sugars were fermented Some may have pink slants and butts (alkaline) = K/K or no color change in the butt = K/NC
35
TSI gas
Positive if the media has been cracked or the butt has lifted from the tube Negative if no gas production
36
TSI sulfate reduction
Positive if media appears black
37
Decarboxylases | Dehydrolases
Media turns yellow- bacteria ferments Glucose which activates the decarboxylase enzymes Then goes to its original color (purple) after the medium becomes alkaline
38
Aseptic technique
Method used to prevent contamination of microorganisms
39
Agar
Inert (bacteria won't eat it) seaweed extract that solidifies at room temperature; gelatin that permits bacterial growth
40
Colony morphology
The appearance of colonies; one colony morphology= same color, shape, elevation
41
Selective medium
Allows one type of microbe to grow, while making others miserable
42
Differential medium
Distinguishes groups of bacteria as they grow on media: you can see a difference
43
Components that make mannitol salt selective
7.5% NaCl
44
Product or characteristic that allows the cell to be visualized by the mannitol salt
Staphylococci can survive in this high % of salt
45
Components that make mannitol salt differential
Mannitol and pH indicators
46
Components that make MacConkeys selective
Crystal violet and bile salts inhibit growth of some organisms (Gram Positive bacteria)
47
Components that make MacConkeys differential
Addition of lactose and pH indicator
48
Differential stain
Shows differences between bacterial cells Ex: Gram positive cells stain purple, Gram negative cells stain pink/ red
49
Primary stain
The first stain used Ex: in gram staining, the the first stain stains all cells purple with crystal violet
50
Mordant
A chemical that fixes in place dye already present Ex: in gram staining, Grams iodine causes violet to clump together or precipitate
51
Counterstain
Used to visualize cells that would otherwise have no stain at the end Ex: in Gram staining, Safrinin is the counterstain
52
Positive control
A given sample that is already known to show a positive result
53
Negative control
A given sample that is already known to show a negative result
54
Vegetative cell
Stain pink; non-endospore forming
55
Hemolysins
Extracellular enzymes that lyse the red blood cells completely
56
Beta hemolysis
Produces a clear zone around the colony
57
Alpha hemolysis
Greenish discoloration around colony indicating incomplete hemolysis
58
Gamma hemolysis
Have no effect on the red cells

Microbiology (17 decks)

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