What type of genome does Corona virus have? Enveloped or not?
Enveloped, +ssRNA (meaning it uses the host polymerase, they are like posttranscriptional eukaryotic mRNA)
What are the coronavirus virulence factors (7!!)
1) S - spike protein 2) HE - hemagg-esterase 3) E - envelope 4) M - membrane 5) N - nucelocapsid 6) RdRp - RNA dep RNA polymerase 7) PLprox+3CLpro
What does S do?
Binding of CoV and fusion of firal membrane with host for entry
What does HE do?
What does E do?
Morphogenesis, assembly, budding, and ion channel activity for replication
What does M do?
Budding and envelope
What does N do?
Binds viral genome + M protein for assembly and budding
What does RdRp do?
Catalyzes replication of RNA for RNA template
What does PLprox and 3CLpro do?
Cleave polyprotein virus
How is CoV transmitted?
Via people; airborne droplets with coughing or sneezing, direct contact with a contaminated surface SARS-CoV = mutated from bats to humans
What season is CoV common and in what group of people?
Children in the winter (DO PEEDS IN SUMMER!
What diseases does CoV cause?
Common Cold: CoV cause mild URI Croup: "croup" cough Pneumonia: uncommon infection of LL in elderly and children SARS: Severe LRI's > pnuemonia by infecting T2 pneumocytes > diffuse alveolar damage > resp failure > ARDS
What are the presentations of the cold and SARS?
Cold > GI Symptoms >>> Neuro symptoms (rare) SARS (2-10 incubation) > Fever > FLS's > Resp symp+GI > resp failure
What is the treatment?
None for HCoV's, ventilatory support and antimicrobials for 2ndary infections
How do you diagnose Cold vs SARS?
SARS = recent travel to China/Taiwain Cold usually self limiting and undiagnosed, RT-PCR
What causes the wide variety of CoV's?
RdRp DOES NOT proofread = mutations